Small Angle Scattering Biological Data Bank SASBDB


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14 hits found for Nucleolysin TIA-1 isoform p40

SASDCC3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA

Nucleolysin TIA-1 isoform p40poly U 15mer experimental SAS data

Nucleolysin TIA-1 isoform p40 poly U 15mer Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein poly U 15mer monomer, 5 kDa RNA
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28

Segmental, Domain-Selective Perdeuteration and Small-Angle Neutron Scattering for Structural Analysis of Multi-Domain Proteins. Angew Chem Int Ed Engl 56(32):9322-9325 (2017)
Sonntag M, Jagtap PKA, Simon B, Appavou MS, Geerlof A, Stehle R, Gabel F, Hennig J, Sattler M

R_g^Guinier	2.4	nm
D_max	8.7	nm
Volume^Porod	39	nm³

SASDCD3 – Nucleolysin TIA-1 isoform p40 (LPQTG containing construct for sortase mediated protein ligation)

Nucleolysin TIA-1 isoform p40 experimental SAS data

Nucleolysin TIA-1 isoform p40 Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1

R_g^Guinier	2.7	nm
D_max	10.3	nm
Volume^Porod	38	nm³

SASDCE3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPQTG containing construct for sortase mediated protein ligation)

poly U 15merNucleolysin TIA-1 isoform p40 experimental SAS data

poly U 15mer Nucleolysin TIA-1 isoform p40 Kratky plot

Sample:	poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 31 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2015 Dec 1

R_g^Guinier	2.4	nm
D_max	8.6	nm
Volume^Porod	40	nm³

SASDCF3 – Nucleolysin TIA-1 isoform p40

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 Apr 28

R_g^Guinier	2.7	nm
D_max	11.2	nm
Volume^Porod	38	nm³

SASDCG3 – Nucleolysin TIA-1 isoform p40 (LPATG containing construct for sortase mediated protein ligation)

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4

R_g^Guinier	2.7	nm
D_max	10.5	nm
Volume^Porod	37	nm³

SASDCH3 – Nucleolysin TIA-1 isoform p40 in complex with U15 RNA (LPATG containing construct for sortase mediated protein ligation)

Sample:	poly U 15mer monomer, 5 kDa RNA Nucleolysin TIA-1 isoform p40 monomer, 30 kDa Homo sapiens protein
Buffer:	10 mM Potassium Phosphate 50 mM NaCl 10 mM DTT, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2016 May 4

R_g^Guinier	2.4	nm
D_max	8.6	nm
Volume^Porod	40	nm³

SASDJK5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to TC1 DNA

Nucleolysin TIA-1 isoform p40TC1 experimental SAS data

Nucleolysin TIA-1 isoform p40 TC1 Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein TC1 monomer, 3 kDa synthetic construct DNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26

...TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA

R_g^Guinier	3.2	nm
D_max	15.1	nm
Volume^Porod	78	nm³

SASDJL5 – Nucleolysin TIA-1 isoform p40 (TIA-1) bound to UC1 RNA

Nucleolysin TIA-1 isoform p40UC1 experimental SAS data

Nucleolysin TIA-1 isoform p40 UC1 Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein UC1 monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Jun 26

...TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA

R_g^Guinier	3.3	nm
D_max	14.4	nm
Volume^Porod	77	nm³

SASDJM5 – ...nucleolysin TIA-1 isoform p40 (TIA-1 APO)

Modified Nucleolysin TIA-1 isofrom p40 experimental SAS data

Modified Nucleolysin TIA-1 isofrom p40 Kratky plot

Sample:	...Nucleolysin TIA-1 isofrom p40 monomer, 42 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 60 mM KCl, 0.5 M arginine-HCl, 1 mM MgCl2, 2 mM DTT, 0.5 mM EDTA, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2020 Jul 7

...TIA-1. Nucleic Acids Res (2021)
Loughlin FE, West DL, Gunzburg MJ, Waris S, Crawford SA, Wilce MCJ, Wilce JA

R_g^Guinier	3.2	nm
D_max	12.7	nm
Volume^Porod	62	nm³

SASDBM6 – Nucleolysin TIA-1 isoform p40

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Jul 2

TIA-1 RRM23 binding and recognition of target oligonucleotides. Nucleic Acids Res 45(8):4944-4957 (2017)
Waris S, García-Mauriño SM, Sivakumaran A, Beckham SA, Loughlin FE, Gorospe M, Díaz-Moreno I, Wilce MCJ, Wilce JA

R_g^Guinier	2.3	nm
D_max	8.8	nm
Volume^Porod	26	nm³

SASDBR6 – Nucleolysin TIA-1 isoform p40 in complex with UUUUUACUCC RNA

Nucleolysin TIA-1 isoform p40RNA (UUUUUACU) experimental SAS data

Nucleolysin TIA-1 isoform p40 RNA (UUUUUACU) Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein RNA (UUUUUACU) monomer, 1 kDa RNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27

R_g^Guinier	2.1	nm
D_max	6.5	nm
Volume^Porod	26	nm³

SASDBQ6 – Nucleolysin TIA-1 isoform p40 in complex with TTTTTACTCC DNA

Nucleolysin TIA-1 isoform p40DNA (TTTTTACTCC) experimental SAS data

Nucleolysin TIA-1 isoform p40 DNA (TTTTTACTCC) Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein DNA (TTTTTACTCC) monomer, 1 kDa DNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27

R_g^Guinier	2.1	nm
D_max	7.1	nm
Volume^Porod	32	nm³

SASDBN6 – Nucleolysin TIA-1 isoform p40 in complex with ACUCCUUUUU RNA

Nucleolysin TIA-1 isoform p40RNA (ACUCCUUUUU) experimental SAS data

Nucleolysin TIA-1 isoform p40 RNA (ACUCCUUUUU) Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein RNA (ACUCCUUUUU) monomer, 1 kDa RNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27

R_g^Guinier	2.2	nm
D_max	6.6	nm
Volume^Porod	30	nm³

SASDBP6 – Nucleolysin TIA-1 isoform p40 in complex with ACTCCTTTTT DNA

Nucleolysin TIA-1 isoform p40DNA (ACTCCTTTTT) experimental SAS data

Nucleolysin TIA-1 isoform p40 DNA (ACTCCTTTTT) Kratky plot

Sample:	Nucleolysin TIA-1 isoform p40 monomer, 21 kDa Homo sapiens protein DNA (ACTCCTTTTT) monomer, 1 kDa DNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 May 27

R_g^Guinier	2.2	nm
D_max	6.7	nm
Volume^Porod	32	nm³

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