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16 hits found for Replicase polyprotein 1ab

SASDPY2 – ...polyprotein monomer

Replicase polyprotein 1ab experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 50 nm3

SASDPZ2 – ...polyprotein dimer

Replicase polyprotein 1ab experimental SAS data
Replicase polyprotein 1ab Kratky plot
Sample: Replicase polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
...polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
Yadav R, Courouble VV, Dey SK, Harrison JJEK, Timm J, Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 110 nm3

SASDSP5 – SARS-CoV-2 Main Protease H163A Mutant - 0.25 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.4 nm
Dmax 7.8 nm
VolumePorod 63 nm3

SASDSQ5 – SARS-CoV-2 Main Protease H163A Mutant - 0.5 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.5 nm
Dmax 8.2 nm
VolumePorod 74 nm3

SASDSR5 – SARS-CoV-2 Main Protease H163A Mutant - 1.0 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDSS5 – SARS-CoV-2 Main Protease H163A Mutant - 3.0 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 102 nm3

SASDST5 – SARS-CoV-2 Main Protease H163A Mutant - 6.3 mg/mL

Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) experimental SAS data
Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) Kratky plot
Sample: Replicase polyprotein 1ab, H3426A (3C-like proteinase nsp5 - H163A mutant) dimer, 67 kDa Severe acute respiratory … protein
Buffer: 25 mM HEPES, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 24
The H163A mutation unravels an oxidized conformation of the SARS-CoV-2 main protease. Nat Commun 14(1):5625 (2023)
Tran N, Dasari S, Barwell SAE, McLeod MJ, Kalyaanamoorthy S, Holyoak T, Ganesan A
RgGuinier 2.6 nm
Dmax 7.7 nm
VolumePorod 98 nm3

SASDKT6 – SARS-CoV-2 non-structural protein 14 (nsp14)

Replicase polyprotein 1ab (non-structural protein 14) experimental SAS data
PYMOL model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.7 nm
Dmax 9.6 nm
VolumePorod 80 nm3

SASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.9 nm
Dmax 12.2 nm
VolumePorod 73 nm3

SASDKV6 – SARS-CoV-2 non-structural protein 10/non-structural protein 16 complex (nsp10/nsp16)

Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3

SASDKW6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14/non-structural protein 16 triplex (nsp10/nsp14/nsp16)

Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)Replicase polyprotein 1ab (non-structural protein 16) experimental SAS data
GASBOR model
Sample: Replicase polyprotein 1ab (non-structural protein 14) monomer, 60 kDa Severe acute respiratory … protein
Replicase polyprotein 1a (non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Replicase polyprotein 1ab (non-structural protein 16) monomer, 33 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 11
Despite the odds: formation of the SARS-CoV-2 methylation complex Nucleic Acids Research (2024)
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dub...
RgGuinier 4.7 nm
Dmax 11.4 nm
VolumePorod 113 nm3

SASDS69 – Full length SARS-CoV-2 Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 21 nm3

SASDS79 – Long SARS-CoV-2 Non-structural protein 10 (Nsp10: C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4386-4392) monomer, 14 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.6 nm
Dmax 5.4 nm
VolumePorod 19 nm3

SASDS89 – Short SARS-CoV-2 Non-structural protein 10 (Nsp10: N- and C-terminal truncation variant)

Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10 - Δ4254-4262; Δ4386-4392) monomer, 13 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Nov 25
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 16 nm3

SASDS99 – Full length SARS Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 16 kDa Severe acute respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.9 nm
VolumePorod 19 nm3

SASDSA9 – Full length MERS Non-structural protein 10 (Nsp10)

Replicase polyprotein 1ab (Non-structural protein 10) experimental SAS data
Replicase polyprotein 1ab (Non-structural protein 10) Kratky plot
Sample: Replicase polyprotein 1ab (Non-structural protein 10) monomer, 15 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES, 300 mM NaCl, 1.5% (v/v) glycerol, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Feb 6
Oligomeric State of β-Coronavirus Non-Structural Protein 10 Stimulators Studied by Small Angle X-ray Scattering International Journal of Molecular Sciences 24(17):13649 (2023)
Knecht W, Fisher S, Lou J, Sele C, Ma S, Rasmussen A, Pinotsis N, Kozielski F
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 19 nm3