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6 hits found for Splicing factor 1

SASDM43 – ...1-ZnF1 tandem domains of RNA-binding protein 5 (C191G mutant)

RNA-binding protein 5 (I107T, C191G) experimental SAS data
Sample: ...107T, C191G) monomer, 14 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on ...16 Sep 26
...splicing factor RBM5. Nat Commun 14(1):4233 (2023)
Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 28 nm3

SASDM53 – ...1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant)

RNA Binding Motif protein 5 (I107T, C191G) experimental SAS data
RNA Binding Motif protein 5 (I107T, C191G) Kratky plot
Sample: ...107T, C191G) monomer, 26 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 6.5
Experiment: SAXS data collected at ...1000, ...1035, Technische Universität München on ...17 Feb 9
...splicing factor RBM5. Nat Commun 14(1):4233 (2023)
Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 36 nm3

SASDM63 – ...1-ZnF1-RRM2 triple domains of RNA-binding protein 5 (C191G mutant) in complex with GGCU_12 RNA

RNA Binding Motif protein 5 (I107T, C191G)Caspase-2 derived RNA GGCU_12 experimental SAS data
OTHER model
Sample: ...107T, C191G) monomer, 26 kDa Homo sapiens protein
...12 monomer, 4 kDa RNA
Buffer: ...100 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at ...1000, ...1035, Technische Universität München on ...17 Mar 1
...splicing factor RBM5. Nat Commun 14(1):4233 (2023)
Soni K, Jagtap PKA, Martínez-Lumbreras S, Bonnal S, Geerlof A, Stehle R, Simon B, Valcárcel J, Sattler M
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 42 nm3

SASDMW7 – ...splicing factor, proline- and glutamine-rich with Non-POU domain-containing octamer-binding protein (SFPQ276-565/NONO53-312)

Splicing factor, proline- and glutamine-rich (276-565)Non-POU domain-containing octamer-binding protein (53-312) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Splicing factor, proline- and glutamine-rich (276-565) monomer, 34 kDa Homo sapiens protein
...12) monomer, 30 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 7.5, 250 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on ...15 Apr 28
...14-598(R542C)/NONO53-312 and the SFPQ276-565/NONO53-312 concentration dependent dimer-tetramer transition
Heidar Koning
RgGuinier 3.0 nm
Dmax 12.7 nm
VolumePorod 102 nm3

SASDSL7 – ...factor 40 homolog A (PRPF40A) in complex with Splicing factor 1 (SF1) C-terminal proline repeat

Pre-mRNA-processing factor 40 homolog ASplicing factor 1 experimental SAS data
Pre-mRNA-processing factor 40 homolog A Splicing factor 1 Kratky plot
Sample: ...factor 40 homolog A monomer, 11 kDa Homo sapiens protein
Splicing factor 1 monomer, 2 kDa Homo sapiens protein
Buffer: ...100 mM NaCl, 0.5 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on ...19
Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs. Nat Commun 15(1):3888 (2024)
Martínez-Lumbreras S, Träger LK, Mulorz MM, Payr M, Dikaya V, Hipp C, König J, Sattler M
RgGuinier 1.9 nm
Dmax 6.9 nm
VolumePorod 19 nm3

SASDUJ4 – ...factor 40 homolog A (PRPF40A) in complex with Splicing factor 1 (SF1) in HEPES buffer

Pre-mRNA-processing factor 40 homolog ASplicing factor 1 experimental SAS data
Pre-mRNA-processing factor 40 homolog A Splicing factor 1 Kratky plot
Sample: ...factor 40 homolog A monomer, 11 kDa Homo sapiens protein
Splicing factor 1 monomer, 2 kDa Homo sapiens protein
Buffer: ...100 mM NaCl, 1 mM DTT, pH: 6.6
Experiment: SAXS data collected at ...1000, Technische Universität München on 2024 Mar 8
Intramolecular autoinhibition regulates the selectivity of PRPF40A tandem WW domains for proline-rich motifs. Nat Commun 15(1):3888 (2024)
Martínez-Lumbreras S, Träger LK, Mulorz MM, Payr M, Dikaya V, Hipp C, König J, Sattler M
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 19 nm3