Small Angle Scattering Biological Data Bank SASBDB


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13 hits found for adenylate cyclase

SASDL62 – ...adenylate cyclase toxin CyaA - construct RTX-1 (amino acids 1132-1294 and 1562-1681 of CyaA)

hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) monomer, 30 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

...adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	41	nm³

SASDL72 – ...adenylate cyclase toxin CyaA - construct RTX-2 (amino acids 1132-1303 and 1562-1681 of CyaA)

hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) experimental SAS data

Sample:	hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) monomer, 31 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1

...adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	37	nm³

SASDJS3 – Adenylate cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	...adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L

R_g^Guinier	4.0	nm
D_max	13.3	nm
Volume^Porod	94	nm³

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V

Sample:	...adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	3.4	nm
D_max	12.1	nm
Volume^Porod	85	nm³

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V

Sample:	...adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	2.7	nm
D_max	8.6	nm
Volume^Porod	45	nm³

SASDB74 – CyaA Block I-V

Adenylate cyclase toxin Block I-V experimental SAS data

Sample:	Adenylate cyclase toxin Block I-V monomer, 70 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P

R_g^Guinier	5.6	nm
D_max	17.2	nm
Volume^Porod	275	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein ...adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDB84 – CyaA Block V

Adenylate cyclase toxin Block V experimental SAS data

Sample:	Adenylate cyclase toxin Block V monomer, 16 kDa Bordetella pertussis protein
Buffer:	10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31

R_g^Guinier	1.8	nm
D_max	5.9	nm
Volume^Porod	24	nm³

SASDGM5 – ...Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data

Sample:	...Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A

R_g^Guinier	4.4	nm
D_max	15.5	nm
Volume^Porod	89	nm³

SASDGN5 – ...Adenylate Cyclase Toxin (CyaA)

Sample:	...Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

R_g^Guinier	8.3	nm
D_max	33.0	nm

SASDU78 – Trypanosoma brucei ESAG4 membrane-proximal Venus Fly Trap domain 2 (VFT2)

Sample:	adenylate cyclase monomer, 43 kDa Trypanosoma brucei protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Sep 23

...adenylate cyclase from Trypanosoma brucei. Mol Biochem Parasitol 260:111653 (2024)
Alves DO, Geens R, da Silva Arruda HR, Jennen L, Corthaut S, Wuyts E, de Andrade GC, Prosdocimi F, Cordeiro Y, Pires JR, Vieira LR, de Oliveira GAP, Sterckx YG, Salmon D

R_g^Guinier	2.3	nm
D_max	7.6	nm
Volume^Porod	76	nm³

SASDCK9 – ...Adenylate Cyclase Toxin (CyaA)

Sample:	...adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDCL9 – ...Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data

Sample:	...adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	78	nm³

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