Browse by ORGANISM: Escherichia coli

SASDK52 – TAF1 tandem bromodomain

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 26
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDK62 – TAF1 tandem bromodomain with inhibitor AZD6738

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 44 nm3

SASDK72 – TAF1 tandem bromodomain with inhibitor BAY299

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 106 nm3

SASDLV2 – Accessory colonization factor SslE at pH 7.4

Accessory colonization factor experimental SAS data
DAMMIN model
Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 4.0 nm
Dmax 14.1 nm
VolumePorod 244 nm3

SASDLW2 – Accessory colonization factor SslE at pH 4.4

Accessory colonization factor experimental SAS data
DAMMIN model
Sample: Accessory colonization factor monomer, 160 kDa Escherichia coli (strain … protein
Buffer: 20 mM citrate-phosphate buffer, 200 mM NaCl, pH: 4.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 27
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 248 nm3

SASDMU6 – The N1 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 17 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDMV6 – The N2 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 23 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 2.2 nm
Dmax 7.7 nm
VolumePorod 42 nm3

SASDMW6 – The N3-M60 domain of accessory colonization factor SslE

Accessory colonization factor experimental SAS data
DAMFILT model
Sample: Accessory colonization factor monomer, 121 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Apr 21
Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation npj Biofilms and Microbiomes 8(1) (2022)
Corsini P, Wang S, Rehman S, Fenn K, Sagar A, Sirovica S, Cleaver L, Edwards-Gayle C, Mastroianni G, Dorgan B, Sewell L, Lynham S, Iuga D, Franks W, Jarvis J, Carpenter G, Curtis M, Bernadó P, Darbari V, Garnett J
RgGuinier 3.4 nm
Dmax 10.5 nm
VolumePorod 171 nm3

SASDM67 – Hemolysin A (HlyA) from Escherichia coli UTI89

Hemolysin, plasmid (Hemolysin A) experimental SAS data
GASBOR model
Sample: Hemolysin, plasmid (Hemolysin A) dimer, 220 kDa Escherichia coli UTI89 protein
Buffer: 100 mM HEPES pH 8.0, 250 mM NaCl, 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 6
Identity Determinants of the Translocation Signal for a Type 1 Secretion System Frontiers in Physiology 12 (2022)
Spitz O, Erenburg I, Kanonenberg K, Peherstorfer S, Lenders M, Reiners J, Ma M, Luisi B, Smits S, Schmitt L
RgGuinier 6.7 nm
Dmax 25.3 nm
VolumePorod 346 nm3

SASDLP4 – Superoxide dismutase, SodA

Superoxide dismutase [Mn] experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Superoxide dismutase [Mn], 23 kDa Escherichia coli (strain … protein
Buffer: 50 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1
Protein quaternary structures in solution are a mixture of multiple forms Chemical Science 13(39):11680-11695 (2022)
Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 54 nm3