Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Escherichia coli

SASDLQ4 – Deoxyribose-phosphate aldolase, DeoC

Deoxyribose-phosphate aldolase experimental SAS data

Sample:	Deoxyribose-phosphate aldolase, 28 kDa Escherichia coli (strain … protein
Buffer:	50 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1

Protein quaternary structures in solution are a mixture of multiple forms Chemical Science 13(39):11680-11695 (2022)
Marciano S, Dey D, Listov D, Fleishman S, Sonn-Segev A, Mertens H, Busch F, Kim Y, Harvey S, Wysocki V, Schreiber G

R_g^Guinier	2.6	nm
D_max	8.0	nm
Volume^Porod	61	nm³

SASDLR4 – 3-oxoacyl-[acyl-carrier-protein] reductase, FabG

3-oxoacyl-[acyl-carrier-protein] reductase FabG experimental SAS data

Sample:	3-oxoacyl-[acyl-carrier-protein] reductase FabG, 26 kDa Escherichia coli (strain … protein
Buffer:	50 mM HEPES, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 1

R_g^Guinier	3.6	nm
D_max	10.6	nm
Volume^Porod	212	nm³

SASDMT3 – NAD Kinase

Sample:	NAD kinase, 33 kDa Escherichia coli (strain … protein
Buffer:	50 mM HEPES, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 2

R_g^Guinier	4.0	nm
D_max	12.5	nm
Volume^Porod	260	nm³

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer

Guanidin-II riboswitch 23mer experimental SAS data

Guanidin-II riboswitch 23mer Kratky plot

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
Schamber T, Binas O, Schlundt A, Wacker A, Schwalbe H

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	14	nm³

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	1.8	nm
D_max	5.8	nm
Volume^Porod	15	nm³

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand

Sample:	Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	2.2	nm
D_max	8.8	nm
Volume^Porod	19	nm³

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer

Guanidin-II riboswitch 49mer experimental SAS data

Guanidin-II riboswitch 49mer Kratky plot

Sample:	Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	4.1	nm
D_max	16.9	nm
Volume^Porod	56	nm³

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Sample:	Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	4.2	nm
D_max	18.1	nm
Volume^Porod	57	nm³

SASDMW9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ and Gdn ligand

Sample:	Guanidin-II riboswitch 49mer hexamer, 95 kDa Escherichia coli RNA
Buffer:	25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment:	SAXS data collected at BM29, ESRF on 2021 Feb 13

R_g^Guinier	8.8	nm
D_max	33.6	nm
Volume^Porod	442	nm³

SASDKG6 – SMP2C2A construct of synaptotagmin-1 (SYT1)

Synaptotagmin-1 (SYT1-SMP2C2A) experimental SAS data

Sample:	Synaptotagmin-1 (SYT1-SMP2C2A) monomer, 72 kDa Escherichia coli protein
Buffer:	20 mM Tris, 100 mM NaCl, 5% glycerol, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 11

The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites Life Science Alliance 4(10):e202101152 (2021)
Benavente J, Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A

R_g^Guinier	4.2	nm
D_max	17.8	nm
Volume^Porod	138	nm³

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