Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Escherichia coli

SASDCY4 – RNase E 603-850

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Dec 5

Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes. Nucleic Acids Res 46(1):387-402 (2018)
Bruce HA, Du D, Matak-Vinkovic D, Bandyra KJ, Broadhurst RW, Martin E, Sobott F, Shkumatov AV, Luisi BF

R_g^Guinier	5.3	nm
D_max	27.5	nm
Volume^Porod	139	nm³

SASDCZ4 – RNase E 603-850/ATP-dependent RNA helicase (RhlB) binary complex

RNase E 603-850ATP-dependent RNA helicase RhlB experimental SAS data

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Feb 11

R_g^Guinier	5.4	nm
D_max	29.5	nm
Volume^Porod	183	nm³

SASDC25 – RNase E 603-850/ATP-dependent RNA helicase (RhlB)/enolase ternary complex

RNase E 603-850ATP-dependent RNA helicase RhlBEnolase experimental SAS data

Sample:	RNase E 603-850 monomer, 30 kDa Escherichia coli protein ATP-dependent RNA helicase RhlB monomer, 47 kDa Escherichia coli protein Enolase dimer, 91 kDa Escherichia coli protein
Buffer:	50 mM Tris HCl, 100 mM NaCl, 100 mM KCl, 10 mM MgCl2, 10 mM DTT and 5 % glycerol (v/v), pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2014 Jul 16

R_g^Guinier	6.4	nm
D_max	30.5	nm
Volume^Porod	280	nm³

SASDC23 – Colicin N WT

Sample:	Colicin N monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDC33 – Colicin N delta 1-39

Colicin N delta 1-39 experimental SAS data

Sample:	Colicin N delta 1-39 monomer, 39 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	60	nm³

SASDC43 – Colicin K145A mutant

Colicin N K145A mutant experimental SAS data

Sample:	Colicin N K145A mutant monomer, 43 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	3.2	nm
D_max	13.6	nm
Volume^Porod	68	nm³

SASDC53 – Colicin N Translocation domain

Colicin N Translocation domain experimental SAS data

Colicin N Translocation domain Rg histogram

Sample:	Colicin N Translocation domain monomer, 10 kDa Escherichia coli protein
Buffer:	50 mM Na-Phosphate 300 mM NaCl, pH: 7.6
Experiment:	SAXS data collected at BM29, ESRF on 2012 Jun 29

The Two-State Prehensile Tail of the Antibacterial Toxin Colicin N. Biophys J 113(8):1673-1684 (2017)
Johnson CL, Solovyova AS, Hecht O, Macdonald C, Waller H, Grossmann JG, Moore GR, Lakey JH

R_g^Guinier	2.8	nm
D_max	11.4	nm
Volume^Porod	22	nm³

SASDE53 – Unlabeled dihydrolipoyllysine-residue succinyltransferase component (BBL) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex experimental SAS data

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex Kratky plot

Sample:	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA

R_g^Guinier	2.1	nm
D_max	8.3	nm
Volume^Porod	41	nm³

SASDE63 – Labeled dihydrolipoyllysine-residue succinyltransferase component (BBL-Alexa488/Alexa594) with denaturant

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexAlexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data

Sample:	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex monomer, 4 kDa Escherichia coli protein Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8

R_g^Guinier	2.3	nm
D_max	10.7	nm
Volume^Porod	13	nm³

SASDE93 – Unlabeled thioredoxin (TRX) with denaturant

Sample:	Thioredoxin 1 monomer, 12 kDa Escherichia coli protein
Buffer:	PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15

R_g^Guinier	3.6	nm
D_max	13.0	nm
Volume^Porod	34	nm³

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