Browse by ORGANISM: Escherichia coli

SASDDB3 – Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 204 nm3

SASDDC3 – Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 May 22
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 218 nm3

SASDDD3 – Human mitochondrial cysteine desulfurase-ISCU-Frataxin (NFS1, ISD11 and Acp heterodimer complex)

Cysteine desulfurase, mitochondrialLYR motif-containing protein 4Acyl carrier proteinIron-sulfur cluster assembly enzyme ISCU, mitochondrialFrataxin, mitochondrial experimental SAS data
HADDOCK model
Sample: Cysteine desulfurase, mitochondrial dimer, 90 kDa Homo sapiens protein
LYR motif-containing protein 4 dimer, 23 kDa Homo sapiens protein
Acyl carrier protein dimer, 22 kDa Escherichia coli protein
Iron-sulfur cluster assembly enzyme ISCU, mitochondrial dimer, 29 kDa Homo sapiens protein
Frataxin, mitochondrial dimer, 29 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, NMRFAM on 2017 Apr 18
Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure 26(8):1127-1136.e4 (2018)
Cai K, Frederick RO, Dashti H, Markley JL
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 287 nm3

SASDDB5 – MsbA in stealth nanodisc (SANS, 100% D2O)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
SASREF CV model
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.0 nm
Dmax 13.0 nm
VolumePorod 189 nm3

SASDDC5 – MsbA in stealth nanodisc (SANS, 100% D2O) + 1 mM ADP

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, 1 mM ADP, pH: 7.5
Experiment: SANS data collected at D11, ILL on 2017 Mar 9
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 3.9 nm
Dmax 12.5 nm
VolumePorod 173 nm3

SASDDD5 – MsbA in stealth nanodisc (SAXS)

Lipid A export ATP-binding/permease protein MsbAMembrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) experimental SAS data
Lipid A export ATP-binding/permease protein MsbA Membrane scaffold protein 1D1 (deuterated, 75%) 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA dimer, 133 kDa Escherichia coli protein
Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein
1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 607 nm3

SASDDE5 – NBD-MsbA (apo)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa Escherichia coli protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 47 nm3

SASDDF5 – NBD-MsbA (+1mM ADP)

Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA experimental SAS data
Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA Kratky plot
Sample: Nucleotide Binding Domain of Lipid A export ATP-binding/permease protein MsbA monomer, 27 kDa Escherichia coli protein
Buffer: 30 mM Tris, 150 mM NaCl, 0.5 mM TCEP, 1 mM ADP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 30
Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure 26(8):1072-1079.e4 (2018)
Josts I, Nitsche J, Maric S, Mertens HD, Moulin M, Haertlein M, Prevost S, Svergun DI, Busch S, Forsyth VT, Tidow H
RgGuinier 2.1 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDB75 – Escherichia coli TraE protein: A VirB8 homolog from plasmid pKM101

Escherichia coli TraE protein (VirB8 homolog) experimental SAS data
GASBOR model
Sample: Escherichia coli TraE protein (VirB8 homolog) hexamer, 171 kDa Escherichia coli protein
Buffer: 50 mM sodium phosphate 300 mM NaCl 40 mM imidazole 0.15 % octyl glucose neopentyl glycol (OGNG), pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Jun 2
VirB8 homolog TraE from plasmid pKM101 forms a hexameric ring structure and interacts with the VirB6 homolog TraD. Proc Natl Acad Sci U S A 115(23):5950-5955 (2018)
Casu B, Mary C, Sverzhinsky A, Fouillen A, Nanci A, Baron C
RgGuinier 4.4 nm
Dmax 13.7 nm
VolumePorod 360 nm3

SASDCZ6 – DtpA SapNP (SapA:POPS) (SEC-SAXS)

saposin-a1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serineDipeptide and tripeptide permease A experimental SAS data
DAMMIF model
Sample: Saposin-a monomer, 9 kDa Homo sapiens protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine, 47 kDa synthetic construct
Dipeptide and tripeptide permease A monomer, 51 kDa Escherichia coli protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 4
Saposin Lipid Nanoparticles: A Highly Versatile and Modular Tool for Membrane Protein Research. Structure 26(2):345-355.e5 (2018)
Flayhan A, Mertens HDT, Ural-Blimke Y, Martinez Molledo M, Svergun DI, Löw C
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 288 nm3