Browse by ORGANISM: Escherichia coli

SASDSC2 – Sulfite reductase truncated linker (ΔAAPSQS) flavoprotein reduced with 10 molar equivalents sodium dithionite

Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) experimental SAS data
Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) Kratky plot
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Δ212-217) octamer, 526 kDa Escherichia coli (strain … protein
Buffer: 50 mM potassium phosphate, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS, Spallation Neutron Source on 2022 Jul 15
Domain crossover in the reductase subunit of NADPH-dependent assimilatory sulfite reductase. J Struct Biol 215(4):108028 (2023)
Walia N, Murray DT, Garg Y, He H, Weiss KL, Nagy G, Elizabeth Stroupe M
RgGuinier 7.6 nm
Dmax 25.0 nm
VolumePorod 952 nm3

SASDQ95 – The complex formed between E. coli S-adenosylmethionine synthase and wild-type S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – The complex formed between E. coli S-adenosylmethionine synthase and S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3

SASDQR4 – Escherichia coli 6S RNA

6S RNA (SsrS gene) experimental SAS data
Escherichia coli 6S RNA Rg histogram
Sample: 6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Buffer: 20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 4
Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Frontiers in Molecular Biosciences 10 (2023)
Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V
RgGuinier 6.1 nm
Dmax 24.0 nm
VolumePorod 175 nm3

SASDQS4 – Escherichia coli 6S:pRNA complex

6S RNA (SsrS gene)product RNA from E. coli 6S experimental SAS data
Escherichia coli 6S:pRNA complex Rg histogram
Sample: 6S RNA (SsrS gene) monomer, 59 kDa Escherichia coli RNA
Product RNA from E. coli 6S monomer, 7 kDa Escherichia coli RNA
Buffer: 20 mM Tris-HCl, 200 mM KCl, 5 mM MgCl2, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 4
Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Frontiers in Molecular Biosciences 10 (2023)
Makraki E, Miliara S, Pagkalos M, Kokkinidis M, Mylonas E, Fadouloglou V
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 260 nm3

SASDQP8 – OxyS sRNA in HEPES buffer pH 6.8, 50 mM NaCl

Stress response regulating small RNA OxyS experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Stress response regulating small RNA OxyS monomer, 36 kDa Escherichia coli RNA
Buffer: 50 mM HEPES pH 6.8, 50 mM NaCl, pH: 6.8
Experiment: SAXS data collected at Austrian SAXS beamline 5.2L, ELETTRA on 2022 Jan 31
Spatial arrangement of functional domains in OxyS stress response sRNA RNA :rna.079618.123 (2023)
Stih V, Amenitsch H, Plavec J, Podbevsek P
RgGuinier 4.8 nm
Dmax 15.5 nm
VolumePorod 68 nm3

SASDKS8 – Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme subunit alpha (anEcTFE-alpha)

Fatty acid oxidation complex subunit alpha experimental SAS data
DAMMIF model
Sample: Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 9
Structural basis for different membrane-binding properties of E. coli anaerobic and human mitochondrial β-oxidation trifunctional enzymes. Structure (2023)
Sah-Teli SK, Pinkas M, Hynönen MJ, Butcher SJ, Wierenga RK, Novacek J, Venkatesan R
RgGuinier 3.3 nm
Dmax 11.2 nm
VolumePorod 145 nm3

SASDRY3 – Kenics GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 28 nm3

SASDRZ3 – Kenics GAC rRNA + Mg: Time-Resolved 32 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 10.0 nm
VolumePorod 27 nm3

SASDR24 – Kenics GAC rRNA + Mg: Time-Resolved 63 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 9.5 nm
VolumePorod 27 nm3