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29 hits found for Gräwert

SASDJ93 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type apo-form

Ubiquitin carboxyl-terminal hydrolase MINDY-2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 45 nm3

SASDJA3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2, wild-type bound to mono-ubiquitin

Ubiquitin carboxyl-terminal hydrolase MINDY-2Polyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase MINDY-2 monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.3 nm
Dmax 6.8 nm
VolumePorod 58 nm3

SASDJB3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to di-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 23
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.5 nm
Dmax 8.0 nm
VolumePorod 68 nm3

SASDJC3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tri-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C trimer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 8.8 nm
VolumePorod 97 nm3

SASDJD3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to tetra-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C tetramer, 34 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.9 nm
Dmax 9.2 nm
VolumePorod 111 nm3

SASDJE3 – Ubiquitin carboxyl-terminal hydrolase, MINDY2 (C266A mutant), bound to penta-ubiquitin

Ubiquitin carboxyl-terminal hydrolase C266A mutantPolyubiquitin-C experimental SAS data
CUSTOM IN-HOUSE model
Sample: Ubiquitin carboxyl-terminal hydrolase C266A mutant monomer, 31 kDa Homo sapiens protein
Polyubiquitin-C pentamer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 26
Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2. Mol Cell (2021)
...Gräwert TW, Knebel A, Svergun DI, Kulathu Y
RgGuinier 2.8 nm
Dmax 9.0 nm
VolumePorod 116 nm3

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

Cholera toxin transcriptional activator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
...Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 16 nm3

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

Transmembrane regulatory protein ToxS experimental SAS data
ALPHAFOLD model
Sample: Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
...Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 55 nm3

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
...Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 51 nm3

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
...Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.4 nm
Dmax 6.6 nm
VolumePorod 60 nm3

SASDNJ5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1)

3-phosphoinositide-dependent protein kinase 1 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.2 nm
VolumePorod 107 nm3

SASDNK5 – Full length 3-phosphoinositide-dependent protein kinase (PDK1) in the presence of HYG8 (2-O-benzoyl-Ins(1,3,4,5,6)P5)

3-phosphoinositide-dependent protein kinase 12-O-benzoyl-Ins(1,3,4,5,6)P5 experimental SAS data
DAMMIF model
Sample: 3-phosphoinositide-dependent protein kinase 1 monomer, 65 kDa Homo sapiens protein
2-O-benzoyl-Ins(1,3,4,5,6)P5 monomer, 1 kDa synthetic construct
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, 1 μM HYG8, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 98 nm3

SASDNL5 – Double mutant catalytic domain of 3-phosphoinositide-dependent protein kinase 1 (PDK1 50–359; Y188G Q292A)

3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) experimental SAS data
SREFLEX model
Sample: 3-phosphoinositide-dependent protein kinase 1 (Y188G Q292A) monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 26
Modulation of the substrate specificity of the kinase PDK1 by distinct conformations of the full-length protein Science Signaling 16(789) (2023)
...Gräwert M, Aramendia P, Leroux A, Potter B, Camacho C, Biondi R
RgGuinier 2.4 nm
Dmax 7.0 nm
VolumePorod 57 nm3

SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data
Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 8
Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...Gräwert TW, Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 50 nm3

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data
DAMMIN model
Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 1.8 nm
Dmax 5.1 nm
VolumePorod 37 nm3

SASDRN9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polysorbate 20 (PS20) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 45 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.4 nm
Dmax 8.6 nm

SASDNW9 – Glucose-regulated protein 78, nucleotide-binding domain

Endoplasmic reticulum chaperone BiP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endoplasmic reticulum chaperone BiP monomer, 42 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 21
Structural basis of CDNF interaction with the UPR regulator GRP78. Nat Commun 15(1):8175 (2024)
...Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 70 nm3

SASDQS6 – Glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with the C-terminal of Cerebral dopamine neurotrophic factor (C-CDNF)

Endoplasmic reticulum chaperone BiPC-terminal of the Cerebral dopamine neurotrophic factor experimental SAS data
SREFLEX model
Sample: Endoplasmic reticulum chaperone BiP monomer, 42 kDa Homo sapiens protein
C-terminal of the Cerebral dopamine neurotrophic factor monomer, 7 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 23
Structural basis of CDNF interaction with the UPR regulator GRP78. Nat Commun 15(1):8175 (2024)
...Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ
RgGuinier 2.4 nm
Dmax 6.9 nm
VolumePorod 66 nm3

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDRQ9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 20-mer, 24 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.0 nm
Dmax 8.0 nm

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDRS9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml)

Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with no or low amount of the fatty acid myristic acid (MA) (< 100 µg/ml) 0, 42 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.3 nm
Dmax 8.3 nm

SASDNX9 – Glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with Cerebral dopamine neurotrophic factor (CDNF)

Endoplasmic reticulum chaperone BiPCerebral dopamine neurotrophic factor experimental SAS data
SREFLEX model
Sample: Endoplasmic reticulum chaperone BiP monomer, 42 kDa Homo sapiens protein
Cerebral dopamine neurotrophic factor monomer, 21 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 21
Structural basis of CDNF interaction with the UPR regulator GRP78. Nat Commun 15(1):8175 (2024)
...Gräwert T, Mamidi S, Kulesskaya N, Evenäs J, Johnsson RE, Svergun D, Bhattacharjee A, Huttunen HJ
RgGuinier 2.8 nm
Dmax 10.0 nm
VolumePorod 75 nm3

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDRP9 – Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polydisperse core-shell ellipsoidal micelles of Polysorbate 20 (PS20) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 45 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 2
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.5 nm
Dmax 8.8 nm

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data
DAMMIN model
Sample: Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 5.4 nm
Dmax 12.5 nm
VolumePorod 679 nm3

SASDRR9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan monolaurate fraction (F2) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 22-mer, 26 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.1 nm
Dmax 8.3 nm

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3

SASDRT9 – Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml)

Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Polydisperse core-shell ellipsoidal micelles of POE sorbitan higher order esters fraction (F4) with high amount of the fatty acid myristic acid (MA) (> 500 µg/ml) 0, 42 kDa
Buffer: aqueous solution of 4% methanol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 29
Small-angle x-ray scattering investigation of the integration of free fatty acids in polysorbate 20 micelles Biophysical Journal (2023)
...Gräwert T, Göddeke H, Konarev P, Svergun D, Nagel N
RgGuinier 3.5 nm
Dmax 8.7 nm