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47 hits found for Larsen

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.2 nm
Dmax 13.9 nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.3 nm
Dmax 13.9 nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.8 nm
Dmax 14.2 nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data
Cobalt/magnesium transport protein CorA Kratky plot
Sample: Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer: 20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Larsen AH, Tidemand FG, Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L
RgGuinier 4.9 nm
Dmax 14.3 nm

SASDEF2 – Mitochondrial import inner membrane translocase complex TIM9·10

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CORAL model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Aug 27
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Larsen K, Wiedemann N, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDEG2 – Mitochondrial import inner membrane translocase complex TIM9·10 in complex with a precursor (GDP/GTP carrier (Ggc1))

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial GTP/GDP carrier protein 1 experimental SAS data
DAMMIF model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 hexamer, 61 kDa Saccharomyces cerevisiae protein
Mitochondrial GTP/GDP carrier protein 1 monomer, 33 kDa Saccharomyces cerevisiae protein
Buffer: 50mM Tris, 150mM NaCl, imidiazole, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
Structural Basis of Membrane Protein Chaperoning through the Mitochondrial Intermembrane Space. Cell 175(5):1365-1379.e25 (2018)
...Larsen K, Wiedemann N, Schanda P
RgGuinier 4.5 nm
Dmax 16.0 nm
VolumePorod 272 nm3

SASDKA4 – Complement factor P, properdin (FP) dimer

Properdin (dimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (dimer) dimer, 110 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 8.1 nm
Dmax 24.0 nm

SASDUA4 – Ribonuclease A Updated Consensus SAXS Data

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
...Larsen A
RgGuinier 1.5 nm
Dmax 5.0 nm

SASDKB4 – Complement factor P, properdin (FP) trimer

Properdin (trimer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (trimer) trimer, 165 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 10.2 nm
Dmax 27.0 nm

SASDUB4 – Urate Oxidase Updated Consensus SAXS Data

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
...Larsen A
RgGuinier 3.2 nm
Dmax 9.3 nm

SASDKC4 – Complement factor P, properdin (FP) tetramer

Properdin (tetramer) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Properdin (tetramer) tetramer, 220 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14
Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
...Larsen K, Arleth L, Andersen GR
RgGuinier 13.1 nm
Dmax 36.0 nm

SASDUC4 – Xylose Isomerase Updated Consensus SAXS Data

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
...Larsen A
RgGuinier 3.3 nm
Dmax 10.1 nm

SASDUD4 – Xylanase Updated Consensus SAXS Data

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
...Larsen A
RgGuinier 1.6 nm
Dmax 5.2 nm

SASDUE4 – Lysozyme Updated Consensus SAXS Data

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
...Larsen A
RgGuinier 1.5 nm
Dmax 4.7 nm

SASDUQ4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 15.2 nm
VolumePorod 367 nm3

SASDUR4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 14.9 nm
VolumePorod 351 nm3

SASDUS4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (i)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDUT4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 5.5 nm
Dmax 27.0 nm

SASDUU4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 31
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 8.8 nm
Dmax 37.0 nm

SASDUV4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iii)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 556 nm3

SASDUW4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iv)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 15.6 nm
Dmax 90.0 nm

SASDUX4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 17.5 nm
VolumePorod 372 nm3

SASDUY4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with HOCPCA

Calcium/calmodulin-dependent protein kinase type II subunit alpha3-hydroxycyclopent-1-enecarboxylic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha 3-hydroxycyclopent-1-enecarboxylic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
3-hydroxycyclopent-1-enecarboxylic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 1 mM HOCPCA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.1 nm
Dmax 12.7 nm
VolumePorod 310 nm3

SASDUZ4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 16.5 nm
VolumePorod 358 nm3

SASDU25 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 15.0 nm
VolumePorod 334 nm3

SASDD35 – Nanodiscs with membrane scaffold protein 1D1 (MSP1D1) and dilaurylphosphatidyl choline (DLPC)

Membrane scaffold protein 1D1 experimental SAS data
Sample: Membrane scaffold protein 1D1 dimer, 50 kDa unidentified protein
Buffer: 20 mM Tris, 100 mM NaCl, 100 mM sodium cholate,, pH: 7.4
Experiment: SAXS data collected at ID14-3, ESRF on 2009 Nov 14
Elliptical structure of phospholipid bilayer nanodiscs encapsulated by scaffold proteins: casting the roles of the lipids and the protein. J Am Chem Soc 132(39):13713-22 (2010)
Skar-Gislinge N, Simonsen JB, Mortensen K, Feidenhans'l R, Sligar SG, Lindberg Møller B, Bjørnholm T, Arleth L
RgGuinier 4.9 nm
Dmax 12.2 nm

SASDU35 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L, Département de Biochimie, Université de Montréal on 2022 Dec 15
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 15.0 nm
VolumePorod 335 nm3

SASDU45 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 329 nm3

SASDU55 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 313 nm3

SASDU65 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 20.0 nm
VolumePorod 375 nm3

SASDU75 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 18.5 nm
VolumePorod 366 nm3

SASDDY5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the resting state (apo), in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.0 nm
Dmax 17.9 nm
VolumePorod 396 nm3

SASDDZ5 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 7.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.4 nm
VolumePorod 407 nm3

SASDD26 – AMPA subtype ionotropic Glutamate receptor GluA2 in the AMPA bound state, in stealth DDM detergents, pH 5.5

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 10 mM AMPA, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 5.5
Experiment: SANS data collected at KWS1, FRM2 on 2017 Sep 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.5 nm
Dmax 18.9 nm
VolumePorod 899 nm3

SASDD36 – AMPA subtype ionotropic Glutamate receptor GluA2 in the GYKI-53655 bound state, in stealth DDM detergents

Glutamate receptor 2 experimental SAS data
Sample: Glutamate receptor 2 monomer, 368 kDa Rattus norvegicus protein
Buffer: D2O based buffer. 1 mM GYKI-53655, 20 mM Tris/DCl, 100 mM NaCl, 0.5 mM deuterated n-dodecyl-β-D-maltopyranoside (synthesized to match out at 100% D2O), pH: 7.5
Experiment: SANS data collected at KWS1, FRM2 on 2016 Oct 19
Small-angle neutron scattering studies on the AMPA receptor GluA2 in the resting, AMPA-bound and GYKI-53655-bound states. IUCrJ 5(Pt 6):780-793 (2018)
Larsen AH, Dorosz J, Thorsen TS, Johansen NT, Darwish T, Midtgaard SR, Arleth L, Kastrup JS
RgGuinier 6.3 nm
Dmax 18.6 nm
VolumePorod 384 nm3

SASDGC6 – Glucuronoyl esterase, deglycolsylated full length

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) experimental SAS data
DAMMIN model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase) monomer, 51 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
The structural basis of fungal glucuronoyl esterase activity on natural substrates. Nat Commun 11(1):1026 (2020)
...Larsen S
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 71 nm3

SASDGD6 – Glucuronoyl esterase S286A, deglycolsylated truncated (amino acids 95-474)

4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 4-O-methyl-glucuronoyl methylesterase (Glucuronoyl esterase, truncated) monomer, 43 kDa Cerrena unicolor protein
Buffer: 20 mM sodium acetate, pH: 5
Experiment: SAXS data collected at Xenocs BioXolver L with GeniX3D, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Oct 10
The structural basis of fungal glucuronoyl esterase activity on natural substrates. Nat Commun 11(1):1026 (2020)
...Larsen S
RgGuinier 2.0 nm
Dmax 6.1 nm
VolumePorod 50 nm3

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: circularized Membrane scaffolding protein 1 E3 D1 , 62 kDa protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer: 20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L
RgGuinier 5.8 nm
Dmax 14.5 nm

SASDH28 – Mitochondrial import inner membrane translocase TIM 9·10·12

Mitochondrial import inner membrane translocase subunit TIM12Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM12 monomer, 12 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM9 dimer, 20 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM MES buffer (2-(N-morpholino)ethanesulfonic acid), pH 6.5, 50 mM NaCl Cloning,, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2017 May 8
Architecture and subunit dynamics of the mitochondrial TIM9·10·12 chaperone (2020)
...Larsen K, Schanda P
RgGuinier 2.7 nm
Dmax 10.0 nm
VolumePorod 103 nm3

SASDTK2 – V2 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V2 variant of the low complexity domain of hnRNPA1 experimental SAS data
V2 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V2 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa synthetic construct protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Larsen K
RgGuinier 2.3 nm
Dmax 8.3 nm
VolumePorod 19 nm3

SASDH89 – Mitochondrial TIM8·13 chaperone in complex with the Tim23 membrane protein precursor

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2015 Apr 24
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDTL2 – V3 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V3 variant of the low complexity domain of hnRNPA1 experimental SAS data
V3 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V3 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Larsen K
RgGuinier 2.3 nm
Dmax 9.3 nm
VolumePorod 18 nm3

SASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23

Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10Mitochondrial import inner membrane translocase subunit TIM23 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM9 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM10 trimer, 31 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM23 monomer, 24 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 May 18
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.2 nm
Dmax 7.7 nm
VolumePorod 146 nm3

SASDTM2 – V4 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V4 variant of the low complexity domain of hnRNPA1 experimental SAS data
V4 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V4 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Larsen K
RgGuinier 2.4 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDJQ4 – Mitochondrial import inner membrane translocase subunits TIM8-TIM13

Mitochondrial import inner membrane translocase subunit TIM8Mitochondrial import inner membrane translocase subunit TIM13 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mitochondrial import inner membrane translocase subunit TIM8 trimer, 29 kDa Saccharomyces cerevisiae protein
Mitochondrial import inner membrane translocase subunit TIM13 trimer, 34 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Structural basis of client specificity in mitochondrial membrane-protein chaperones. Sci Adv 6(51) (2020)
...Larsen K, Bersch B, Schanda P
RgGuinier 3.1 nm
Dmax 10.9 nm
VolumePorod 136 nm3

SASDTN2 – V5 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V5 variant of the low complexity domain of hnRNPA1 experimental SAS data
V5 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V5 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Larsen K
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 19 nm3

SASDTJ2 – Low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 experimental SAS data
Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 Kratky plot
Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Larsen K
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 19 nm3