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19 hits found for Longhi

SASDUG3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 50 nm3

SASDUH3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Endoglucanase-7 experimental SAS data
Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer Rg histogram
Sample: Endoglucanase-7 dimer, 60 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Dec 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 4.8 nm
Dmax 21.7 nm
VolumePorod 83 nm3

SASDUJ3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) deltaX283

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 27 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 1.8 nm
Dmax 6.3 nm
VolumePorod 32 nm3

SASDUK3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) C286A*

Endoglucanase-7 (C286A) experimental SAS data
Endoglucanase-7 (C286A) Kratky plot
Sample: Endoglucanase-7 (C286A) monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 11.6 nm
VolumePorod 48 nm3

SASDUL3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 monomer, 30 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 4.1 nm
Dmax 14.6 nm
VolumePorod 85 nm3

SASDUM3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 dimer, 61 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 6.2 nm
Dmax 24.5 nm
VolumePorod 206 nm3

SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 3.9 nm
Dmax 17.0 nm
VolumePorod 68 nm3

SASDUP3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) dimer

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 dimer, 66 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
...Longhi S, Berrin JG
RgGuinier 6.4 nm
Dmax 27.9 nm
VolumePorod 199 nm3

SASDUQ3 – C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV)

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV) Rg histogram
Sample: Protein W monomer, 6 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
...Longhi S
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 8 nm3

SASDUR3 – C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV)

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV) Rg histogram
Sample: Protein W monomer, 6 kDa Henipavirus nipahense protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
...Longhi S
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 8 nm3

SASDJB5 – Nipah virus phosphoprotein, N-terminal amino acids 1-406 (PNT)

Phosphoprotein experimental SAS data
Nipah virus phosphoprotein, N-terminal amino acids 1-406 (PNT) Rg histogram
Sample: Phosphoprotein monomer, 45 kDa Nipah henipavirus protein
Buffer: 20 mM Tris-HCl, 0.3 M NaCl, 5 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 May 3
Ensemble description of the intrinsically disordered N-terminal domain of the Nipah virus P/V protein from combined NMR and SAXS. Sci Rep 10(1):19574 (2020)
...Longhi S
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 210 nm3

SASDQB7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
...Longhi S
RgGuinier 3.7 nm
Dmax 19.0 nm
VolumePorod 48 nm3

SASDQC7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region) mutated YYY --> AAA

Non-structural protein V (Y211A, Y212A, Y213A mutant) experimental SAS data
Non-structural protein V (Y211A, Y212A, Y213A mutant) Kratky plot
Sample: Non-structural protein V (Y211A, Y212A, Y213A mutant) monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
...Longhi S
RgGuinier 4.0 nm
Dmax 19.0 nm
VolumePorod 49 nm3

SASDQD7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region, C-terminal truncated)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 9 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
...Longhi S
RgGuinier 2.8 nm
Dmax 13.0 nm
VolumePorod 22 nm3

SASDQE7 – Region within the N-terminal domain of P/V/W protein of Hendra virus (PNT3 region C-terminal truncated), mutated YYY --> AAA

Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) experimental SAS data
Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) Kratky plot
Sample: Non-structural protein V (ΔC-terminal and Y111A, Y112A, Y113A mutant) monomer, 9 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
...Longhi S
RgGuinier 2.7 nm
Dmax 12.5 nm
VolumePorod 21 nm3

SASDQF7 – Region within N-terminal domain of P/V/W protein of Nipah virus (PNT3 region)

Non-structural protein V experimental SAS data
Non-structural protein V Kratky plot
Sample: Non-structural protein V monomer, 15 kDa Nipah henipavirus protein
Buffer: 50 mM sodium phosphate, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Molecular Determinants of Fibrillation in a Viral Amyloidogenic Domain from Combined Biochemical and Biophysical Studies International Journal of Molecular Sciences 24(1):399 (2022)
...Longhi S
RgGuinier 3.7 nm
Dmax 16.5 nm
VolumePorod 47 nm3

SASDLF9 – 200-310 region of Hendra virus P/V/W protein (PNT3)

Protein W experimental SAS data
200-310 region of Hendra virus P/V/W protein (PNT3) Rg histogram
Sample: Protein W monomer, 15 kDa Hendra virus (isolate … protein
Buffer: 50 mM sodium phosphate, 5 mM EDTA, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Identification of a Region in the Common Amino-terminal Domain of Hendra Virus P, V, and W Proteins Responsible for Phase Transition and Amyloid Formation Biomolecules 11(9):1324 (2021)
...Longhi S
RgGuinier 3.4 nm
Dmax 15.5 nm
VolumePorod 38 nm3

SASDLK9 – Hendra virus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Hendra virus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
...Longhi S
RgGuinier 7.2 nm
Dmax 24.0 nm
VolumePorod 338 nm3

SASDLL9 – Nipah henipavirus W protein in 1 M urea, 5 mM DTT

Protein W experimental SAS data
Nipah henipavirus W protein in 1 M urea, 5 mM DTT Rg histogram
Sample: Protein W monomer, 53 kDa Nipah henipavirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 M urea, 5 mM DTT, pH: 7
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Jun 12
Experimental Evidence of Intrinsic Disorder and Amyloid Formation by the Henipavirus W Proteins International Journal of Molecular Sciences 23(2):923 (2022)
...Longhi S
RgGuinier 7.1 nm
Dmax 24.5 nm
VolumePorod 327 nm3