Small Angle Scattering Biological Data Bank SASBDB


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44 hits found for Pedersen

SASDM42 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles without Mg2+

Cobalt/magnesium transport protein CorA experimental SAS data

Cobalt/magnesium transport protein CorA Kratky plot

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation. Elife 11 (2022)
...Pedersen MC, Huda P, Berndtsson J, Darwish T, Yepuri NR, Martel A, Pomorski TG, Bertarello A, Sansom M, Rapp M, Crehuet R, Schubeis T, Lindorff-Larsen K, Pintacuda G, Arleth L

R_g^Guinier	4.2	nm
D_max	13.9	nm

SASDM52 – Cobalt/magnesium transport protein CorA in matched-out deuterated dodecylmaltoside (dDDM) micelles with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.3	nm
D_max	13.9	nm

SASDM62 – Cobalt/magnesium transport protein CorA in matched-out deuterated nanodiscs without Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 1 mM EDTA-NaOD, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.8	nm
D_max	14.2	nm

SASDM72 – Cobalt/magnesium transport protein CorA in match-out deuterated nanodiscs with bound Mg2+

Sample:	Cobalt/magnesium transport protein CorA pentamer, 208 kDa Thermotoga maritima (strain … protein
Buffer:	20 mM Tris-DCl, 150 mM NaCl, 40 mM MgCl2, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at D22, Institut Laue-Langevin (ILL) on 2018 Jun 23

R_g^Guinier	4.9	nm
D_max	14.3	nm

SASDJK3 – Native alpha-2-macroglobulin, glycosylated

Alpha-2-macroglobulin experimental SAS data

Sample:	Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2020 Jan 22

Structural Investigations of Human A2M Identify a Hollow Native Conformation That Underlies Its Distinctive Protease-Trapping Mechanism. Mol Cell Proteomics 20:100090 (2021)
...Pedersen JS, Enghild JJ

R_g^Guinier	7.7	nm
D_max	22.7	nm

SASDJL3 – Methylamine-treated alpha-2-macroglobulin, glycosylated

Sample:	Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2020 Jan 22

R_g^Guinier	6.7	nm
D_max	19.7	nm

SASDJM3 – Trypsin-treated alpha-2-macroglobulin, glycosylated

Cationic trypsinAlpha-2-macroglobulin experimental SAS data

Sample:	Cationic trypsin dimer, 52 kDa Bos taurus protein Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2020 Jan 15

R_g^Guinier	6.6	nm
D_max	19.7	nm

SASDJN3 – Native alpha-2-macroglobulin, deglycosylated

Sample:	Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2019 Apr 12

R_g^Guinier	7.6	nm
D_max	20.2	nm

SASDJP3 – Methylamine-treated alpha-2-macroglobulin, deglcycosylated

Sample:	Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2019 Apr 13

R_g^Guinier	6.6	nm
D_max	19.7	nm

SASDJQ3 – Trypsin-treated alpha-2-macroglobulin, deglycosylated

Sample:	Cationic trypsin dimer, 52 kDa Bos taurus protein Alpha-2-macroglobulin tetramer, 643 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar w Excillum source, Department of Chemistry, iNANO building, Aarhus Uinversity on 2020 Jan 22

R_g^Guinier	6.6	nm
D_max	20.7	nm

SASDKA4 – Complement factor P, properdin (FP) dimer

Sample:	Properdin (dimer) dimer, 110 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14

Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR

R_g^Guinier	8.1	nm
D_max	24.0	nm

SASDKB4 – Complement factor P, properdin (FP) trimer

Properdin (trimer) experimental SAS data

Sample:	Properdin (trimer) trimer, 165 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14

Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR

R_g^Guinier	10.2	nm
D_max	27.0	nm

SASDKC4 – Complement factor P, properdin (FP) tetramer

Properdin (tetramer) experimental SAS data

Sample:	Properdin (tetramer) tetramer, 220 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Nov 14

Properdin oligomers adopt rigid extended conformations supporting function. Elife 10 (2021)
Pedersen DV, Pedersen MN, Mazarakis SM, Wang Y, Lindorff-Larsen K, Arleth L, Andersen GR

R_g^Guinier	13.1	nm
D_max	36.0	nm

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS

Ribonuclease pancreatic experimental SAS data

Sample:	Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
...Pedersen M, Skou S, Arleth L, Vestergaard B

R_g^Guinier	1.5	nm
D_max	4.8	nm
Volume^Porod	10	nm³

SASDEZ4 – Carbonic Anhydrase from in-house SEC-SAXS

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24

R_g^Guinier	1.9	nm
D_max	5.3	nm
Volume^Porod	38	nm³

SASDE25 – Human insulin hexamer from in-house SEC-SAXS

Sample:	Human insulin hexamer, 35 kDa Homo sapiens protein
Buffer:	7 mM sodium phosphate, 60 mM phenol, 200 µM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 26

R_g^Guinier	2.0	nm
D_max	5.9	nm
Volume^Porod	39	nm³

SASDE35 – Ovalbumin monomer from in-house SEC-SAXS

Sample:	Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24

R_g^Guinier	2.4	nm
D_max	7.8	nm
Volume^Porod	55	nm³

SASDE45 – Conalbumin monomer from in-house SEC-SAXS

Sample:	Ovotransferrin monomer, 76 kDa Gallus gallus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24

R_g^Guinier	3.0	nm
D_max	10.1	nm
Volume^Porod	100	nm³

SASDE55 – Apoferritin monomer from in-house SEC-SAXS

Ferritin light chain experimental SAS data

Sample:	Ferritin light chain 24-mer, 476 kDa Equus caballus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24

R_g^Guinier	5.1	nm
D_max	12.3	nm
Volume^Porod	685	nm³

SASDE65 – Bovine serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.8	nm
D_max	9.2	nm
Volume^Porod	92	nm³

SASDE75 – Bovine serum albumin monomer from in-house SEC-SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.8	nm
D_max	9.9	nm
Volume^Porod	104	nm³

SASDE85 – Bovine serum albumin monomer from in-house SEC-SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.8	nm
D_max	8.2	nm
Volume^Porod	85	nm³

SASDE95 – Bovine serum albumin monomer from in-house SEC-SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	2.9	nm
D_max	8.6	nm
Volume^Porod	74	nm³

SASDEA5 – Bovine serum albumin monomer from in-house SEC-SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 25

R_g^Guinier	2.8	nm
D_max	9.6	nm
Volume^Porod	78	nm³

SASDEB5 – Bovine serum albumin from in-house SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 May 4

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	99	nm³

SASDEC5 – Bovine serum albumin dimer from in-house SEC-SAXS

Sample:	Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer:	PBS, pH: 7.4
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23

R_g^Guinier	4.1	nm
D_max	13.8	nm
Volume^Porod	200	nm³

SASDED5 – Bovine serum albumin monomer from synchrotron SEC-SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	PBS + 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jun 14

R_g^Guinier	2.7	nm
D_max	8.7	nm
Volume^Porod	84	nm³

SASDEE5 – Bovine serum albumin from synchrotron SAXS

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	85	nm³

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data

Sample:	circularized Membrane scaffolding protein 1 E3 D1 , 62 kDa protein 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer:	20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 5

Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
...Pedersen M, Arleth L

R_g^Guinier	5.8	nm
D_max	14.5	nm

SASDBZ7 – Complement factor 1s in complex with Complement factor 1r

Complement C1r subcomponentComplement C1s subcomponent experimental SAS data

Sample:	Complement C1r subcomponent dimer, 156 kDa Homo sapiens protein Complement C1s subcomponent dimer, 150 kDa Homo sapiens protein
Buffer:	50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Dec 8

Structure and activation of C1, the complex initiating the classical pathway of the complement cascade. Proc Natl Acad Sci U S A 114(5):986-991 (2017)
...Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR

R_g^Guinier

11.6

SASDB28 – Complement factor 1q (C1q)

Complement C1q subcomponent subunit CComplement C1q subcomponent subunit BComplement C1q subcomponent subunit A experimental SAS data

Complement C1q subcomponent subunit C Complement C1q subcomponent subunit B Complement C1q subcomponent subunit A Kratky plot

Sample:	Complement C1q subcomponent subunit C hexamer, 142 kDa Homo sapiens protein Complement C1q subcomponent subunit B hexamer, 142 kDa Homo sapiens protein Complement C1q subcomponent subunit A hexamer, 142 kDa Homo sapiens protein
Buffer:	50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2014 Dec 8

R_g^Guinier

12.6

SASDB38 – Inactivated complement factor 1 (C1)

Complement C1q subcomponent subunit CComplement C1q subcomponent subunit BComplement C1q subcomponent subunit AComplement C1r subcomponentComplement C1s subcomponent experimental SAS data

Sample:	Complement C1q subcomponent subunit C hexamer, 142 kDa Homo sapiens protein Complement C1q subcomponent subunit B hexamer, 142 kDa Homo sapiens protein Complement C1q subcomponent subunit A hexamer, 142 kDa Homo sapiens protein Complement C1r subcomponent dimer, 156 kDa Homo sapiens protein Complement C1s subcomponent dimer, 150 kDa Homo sapiens protein
Buffer:	50 mM EPPS, 145 mM NaCl, 3 mM CaCl2, pH: 8.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16

R_g^Guinier	11.5	nm
D_max	36.6	nm

SASDB59 – Recombinant monomeric human Properdin

Human recombinant Properdin TSR 0-3Human recombinant Properdin TSR 4-6 experimental SAS data

Sample:	Human recombinant Properdin TSR 0-3 monomer, 25 kDa Homo sapiens protein Human recombinant Properdin TSR 4-6 monomer, 24 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16

Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
Pedersen DV, Roumenina L, Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR

R_g^Guinier	5.1	nm
D_max	18.0	nm

SASDB69 – E244K monomeric human Properdin

E244K Human Properdin experimental SAS data

Sample:	E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer:	10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27

R_g^Guinier	4.1	nm
D_max	15.0	nm

SASDEL9 – Escherichia coli aerobic fatty acid beta-oxidation trifunctional enzyme complex

Fatty acid oxidation complex subunit alphaFatty acid oxidation complex subunit alpha3-ketoacyl-CoA thiolase FadA (beta subunit) experimental SAS data

Sample:	Fatty acid oxidation complex subunit alpha monomer, 81 kDa Escherichia coli protein Fatty acid oxidation complex subunit alpha monomer, 81 kDa Escherichia coli protein 3-ketoacyl-CoA thiolase FadA (beta subunit) dimer, 82 kDa Escherichia coli protein
Buffer:	20 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 120 mM KCl, 2.5 mM DTT, pH: 7.2
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 May 30

Complementary substrate specificity and distinct quaternary assembly of the Escherichia coli aerobic and anaerobic beta-oxidation trifunctional enzyme complexes. Biochem J (2019)
...Pedersen JS, Butcher SJ, Wierenga RK, Venkatesan R

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	406	nm³

SASDEM9 – anEcTFE: Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme complex

Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic Fatty acid oxidation complex subunit alphaanaerobic 3-ketoacyl-CoA thiolase FadI beta subunitanaerobic 3-ketoacyl-CoA thiolase FadI beta subunit experimental SAS data

Sample:	Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli (strain … protein anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein anaerobic Fatty acid oxidation complex subunit alpha monomer, 77 kDa Escherichia coli protein anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli protein anaerobic 3-ketoacyl-CoA thiolase FadI beta subunit dimer, 96 kDa Escherichia coli protein
Buffer:	50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2015 Sep 22

R_g^Guinier	6.2	nm
D_max	19.6	nm
Volume^Porod	856	nm³

SASDJU9 – Mammalian cell entry protein 1A (Mce1A36-148)

Mce-family protein Mce1A monomer experimental SAS data

Sample:	Mce-family protein Mce1A monomer monomer, 17 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis IUCrJ 8(5) (2021)
...Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R

R_g^Guinier	2.1	nm
D_max	8.7	nm
Volume^Porod	29	nm³

SASDK32 – Mammalian cell entry protein 1A (Mce1A38-454)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.3	nm
D_max	21.6	nm
Volume^Porod	362	nm³

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

Sample:	Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer:	50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	216	nm³

SASDJZ9 – Mammalian cell entry protein 1A (Mce1A38-325)

Sample:	Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein n-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
Buffer:	50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.6	nm
D_max	17.5	nm
Volume^Porod	151	nm³

SASDJV9 – Mammalian cell entry protein 4A (Mce4A39-140)

Mce-family protein Mce4A experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 15 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM MOPS, 350mM NaCl, 10% Glycerol, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 10

R_g^Guinier	2.1	nm
D_max	7.8	nm
Volume^Porod	22	nm³

SASDJW9 – Mammalian cell entry protein 4A (Mce4A36-400)

Mce-family protein Mce4An-Dodecyl-β-D-Maltopyranoside experimental SAS data

Sample:	Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 13

R_g^Guinier	5.7	nm
D_max	21.5	nm
Volume^Porod	446	nm³

SASDJX9 – Mammalian cell entry protein 4A (Mce4A121-400)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data

Sample:	n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Nov 28

R_g^Guinier	5.0	nm
D_max	19.1	nm
Volume^Porod	278	nm³

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

Sample:	n-Dodecyl-β-D-Maltopyranoside 0, 102 kDa Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 May 2

R_g^Guinier	4.8	nm
D_max	18.2	nm
Volume^Porod	189	nm³

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