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8 hits found for Werten

SASDN75 – TetR/AcrR-like repressor SCO3201 from Streptomyces coelicor

Putative tetR-family transcriptional regulator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Putative tetR-family transcriptional regulator dimer, 47 kDa Streptomyces coelicolor (strain … protein
Buffer: 20 mM Tris/HCl, 400 mM NaCl, 50 mM imidazole, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2014 May 2
Crystal Structures of Free and Ligand-Bound Forms of the TetR/AcrR-Like Regulator SCO3201 from Streptomyces coelicolor Suggest a Novel Allosteric Mechanism. FEBS J (2022)
Werten S, Waack P, Palm GJ, Virolle MJ, Hinrichs W
RgGuinier 2.6 nm
Dmax 6.5 nm
VolumePorod 77 nm3

SASDFD5 – Tetracycline repressor (class D), TetR(D)

Tetracycline repressor (class D) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tetracycline repressor (class D) dimer, 47 kDa Escherichia coli protein
Buffer: 50 mM Tris/HCl 150 mM NaCl 10 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Thermodynamics, cooperativity and stability of the tetracycline repressor (TetR) upon tetracycline binding. Biochim Biophys Acta Proteins Proteom :140404 (2020)
...Werten S, Girbardt B, Berndt L, Delcea M, Hinrichs W
RgGuinier 2.6 nm
Dmax 7.7 nm
VolumePorod 85 nm3

SASDFE5 – Tetracycline repressor (class D), TetR(D), in complex with 5a,6-anhydrotetracycline (ATc)

Tetracycline repressor (class D)5a,6-anhydrotetracycline experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tetracycline repressor (class D) dimer, 47 kDa Escherichia coli protein
5a,6-anhydrotetracycline dimer, 1 kDa
Buffer: 50 mM Tris/HCl 150 mM NaCl 10 mM MgCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Thermodynamics, cooperativity and stability of the tetracycline repressor (TetR) upon tetracycline binding. Biochim Biophys Acta Proteins Proteom :140404 (2020)
...Werten S, Girbardt B, Berndt L, Delcea M, Hinrichs W
RgGuinier 2.6 nm
Dmax 6.8 nm
VolumePorod 77 nm3

SASDF76 – Polyphosphate-targeting protein A

Polyphosphate-targeting protein A experimental SAS data
CORAL model
Sample: Polyphosphate-targeting protein A dimer, 79 kDa Streptomyces chartreusis protein
Buffer: 20 mM Tris-HCl 400 mM NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 24
Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold. FEBS Lett (2019)
Werten S, Rustmeier NH, Gemmer M, Virolle MJ, Hinrichs W
RgGuinier 3.5 nm
Dmax 11.8 nm
VolumePorod 124 nm3

SASDKA6 – Flavonoid-responsive regulator A (FrrA)

TetR/AcrR family transcriptional regulator experimental SAS data
CORAL model
Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
The Induction Mechanism of the Flavonoid-Responsive Regulator FrrA. FEBS J (2021)
...Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.4 nm
Dmax 9.0 nm
VolumePorod 93 nm3

SASDKB6 – Flavonoid-responsive regulator A (FrrA) in the presence of genistein

TetR/AcrR family transcriptional regulator5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one experimental SAS data
TetR/AcrR family transcriptional regulator 5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one Kratky plot
Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one monomer, 0 kDa
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 0.08 mM genistein, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 27
The Induction Mechanism of the Flavonoid-Responsive Regulator FrrA. FEBS J (2021)
...Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.4 nm
Dmax 8.7 nm
VolumePorod 96 nm3

SASDKC6 – Flavonoid-responsive regulator A (FrrA) in the presence of naringenin

TetR/AcrR family transcriptional regulator5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one experimental SAS data
TetR/AcrR family transcriptional regulator 5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one Kratky plot
Sample: TetR/AcrR family transcriptional regulator dimer, 48 kDa Bradyrhizobium diazoefficiens protein
5,7-dihydroxy-2-(4-hydroxyphenyl)chroman-4-one monomer, 0 kDa
Buffer: 20 mM Na2HPO4, 50 mM NaCl, 50 mM imidazole, 1 mM naringenin, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
The Induction Mechanism of the Flavonoid-Responsive Regulator FrrA. FEBS J (2021)
...Werten S, Hoppen J, Palm GJ, Göttfert M, Hinrichs W
RgGuinier 3.2 nm
Dmax 8.6 nm
VolumePorod 91 nm3

SASDA58 – UL26N of pseudorabies virus

VP24 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: VP24 dimer, 53 kDa Suid herpesvirus 1 protein
Buffer: 50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease. PLoS Pathog 11(7):e1005045 (2015)
...Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W
RgGuinier 2.6 nm
Dmax 10.6 nm
VolumePorod 73 nm3