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52 hits found for Yen-Lin Chen

SASDKF2 – Mixed-sequence DNA dimer (25 base pair DNA)

Mixed-sequence 25 base-paired DNA experimental SAS data
Mixed-sequence 25 base-paired DNA Kratky plot
Sample: Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 17 nm3

SASDHG2 – 12 base-paired RNA double helix (RNA12) with 30 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 30 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDKG2 – AT-sequence DNA dimer (25 base pair DNA)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 16 nm3

SASDHH2 – 12 base-paired RNA double helix (RNA12) with 50 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 50 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDHJ2 – 12 base-paired RNA double helix (RNA12) with 100 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 100 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.5 nm

SASDHK2 – 12 base-paired RNA double helix (RNA12) with 200 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 200 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHL2 – 12 base-paired RNA double helix (RNA12) with 500 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 500 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHM2 – 12 base-paired RNA double helix (RNA12) with 0.25 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 0.25 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDHN2 – 12 base-paired RNA double helix (RNA12) with 1 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 1.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 5.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDHQ2 – 25 base-paired RNA double helix (RNA25) with 100 mM NaCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.6 nm

SASDHS2 – 25 base-paired RNA double helix (RNA25) with 400 mM KCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.3 nm

SASDHT2 – 25 base-paired DNA double helix (DNA25) with 400 mM KCl - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDHU2 – 25 base-paired DNA double helix (DNA25) with 0.5 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 0.5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 2.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.5 nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data
A dENE + Poly(A)30 Kratky plot
Sample: A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 13.3 nm

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

B dENE experimental SAS data
B dENE Kratky plot
Sample: B dENE monomer, 24 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.8 nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data
B dENE + Poly(A)30 Kratky plot
Sample: B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

C dENE experimental SAS data
C dENE Kratky plot
Sample: C dENE monomer, 29 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.4 nm
Dmax 12.4 nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Xtal dENE experimental SAS data
Xtal dENE Kratky plot
Sample: Xtal dENE monomer, 28 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.1 nm
Dmax 11.0 nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDKG5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKH5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKJ5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKK5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKL5 – RNA Short Tetraloop Hairpin Triplex

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 6.3 nm

SASDKM5 – Metastasis-Associated Lung Adenocarcinoma Transcript 1

Metastasis-Associated Lung Adenocarcinoma Transcript 1 experimental SAS data
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Kratky plot
Sample: Metastasis-Associated Lung Adenocarcinoma Transcript 1 monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 μM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 7.9 nm

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA experimental SAS data
30nt Single-stranded RNA Kratky plot
Sample: 30nt Single-stranded RNA monomer, 10 kDa RNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.2 nm
Dmax 8.2 nm

SASDKP5 – RNA Short Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKQ5 – RNA Short Tetraloop Hairpin Duplex in 200 mM KCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.0 nm
Dmax 6.8 nm

SASDKR5 – RNA Short Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.1 nm
Dmax 7.8 nm

SASDKS5 – RNA Short Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDKT5 – RNA Short Tetraloop Hairpin Triplex in 200 mM KCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.6 nm

SASDKU5 – RNA Short Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm

SASDKV5 – RNA Long Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 9.8 nm

SASDKW5 – RNA Long Tetraloop Hairpin Duplex in 200 mM KCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.0 nm

SASDKX5 – RNA Long Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.3 nm

SASDKY5 – RNA Long Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 10.3 nm

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 18.2 nm