Small Angle Scattering Biological Data Bank SASBDB


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10 hits found for Calmodulin-1

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein calcium ions tetramer, 0 kDa Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer:	50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	55	nm³

SASDNX3 – Calcium-bound Calmodulin, including structural models

Calcium-bound Calmodulin, including structural models Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	33	nm³

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

Calmodulin-1Calmidazolium experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Calmidazolium monomer, 1 kDa
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

R_g^Guinier	1.7	nm
D_max	5.2	nm
Volume^Porod	30	nm³

SASDJ64 – Calcium bound Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.3	nm
Volume^Porod	27	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDPV6 – Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

Binding by calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	38	nm³

SASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)

Calmodulin-1Pre-mRNA-processing factor 40 homolog A experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

R_g^Guinier	2.4	nm
D_max	8.7	nm
Volume^Porod	29	nm³

SASDPD4 – Ca2+-calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4

New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H

R_g^Guinier	2.1	nm
D_max	6.9	nm
Volume^Porod	23	nm³

SASDPF4 – P2X7 ballast domain in complex with Ca2+-calmodulin

Calmodulin-1P2X purinoceptor 7 experimental SAS data

Calmodulin-1 P2X purinoceptor 7 Kratky plot

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4

New insights into P2X7 receptor regulation: Ca2+-calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H

R_g^Guinier	3.2	nm
D_max	13.5	nm
Volume^Porod	73	nm³

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