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9 hits found for S-adenosyl-L-methionine

SASDQ95 – ...S-adenosyl-L-methionine lyase from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Jun 17
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.5 nm
VolumePorod 325 nm3

SASDQA5 – ...S-adenosyl-L-methionine lyase mutant E68Q/Q94A from bacteriophage T3

S-adenosylmethionine synthaseS-adenosyl-L-methionine lyase (E68Q/Q94A) experimental SAS data
OTHER model
Sample: S-adenosylmethionine synthase tetramer, 168 kDa Escherichia coli (strain … protein
S-adenosyl-L-methionine lyase (E68Q/Q94A) tetramer, 71 kDa Enterobacteria phage T3 protein
Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM β-mercaptoethanol, 2 % glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 May 7
Phage T3 overcomes the BREX defense through SAM cleavage and inhibition of SAM synthesis by SAM lyase. Cell Rep 42(8):112972 (2023)
Andriianov A, Trigüis S, Drobiazko A, Sierro N, Ivanov NV, Selmer M, Severinov K, Isaev A
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 326 nm3

SASDJS5 – ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Expression and analysis of the SAM-dependent RNA methyltransferase Rsm22 from Saccharomyces cerevisiae Acta Crystallographica Section D Structural Biology 77(6) (2021)
Alam J, Rahman F, Sah-Teli S, Venkatesan R, Koski M, Autio K, Hiltunen J, Kastaniotis A
RgGuinier 3.8 nm
Dmax 13.9 nm
VolumePorod 160 nm3

SASDJT5 – ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
Expression and analysis of the SAM-dependent RNA methyltransferase Rsm22 from Saccharomyces cerevisiae Acta Crystallographica Section D Structural Biology 77(6) (2021)
Alam J, Rahman F, Sah-Teli S, Venkatesan R, Koski M, Autio K, Hiltunen J, Kastaniotis A
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDJ26 – ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-monomer

Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial monomer, 70 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 3.8 nm
Dmax 13.9 nm
VolumePorod 160 nm3

SASDJ36 – ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial-dimer

dimeric Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial experimental SAS data
DAMMIN model
Sample: ...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial dimer, 141 kDa Saccharomyces cerevisiae protein
Buffer: 40 mM Tris pH 7.5, 500 mM NaCl, 5% glycerol, 2.5 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 May 1
...S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22
Jahangir Alam
RgGuinier 5.0 nm
Dmax 18.2 nm
VolumePorod 516 nm3

SASDSL9 – ...S-adenosyl-L-methionine (SAM)

SAM riboswitch element A aptamer domain (RNA)S-adenosyl-L-methionine experimental SAS data
SAM riboswitch element A aptamer domain (RNA) S-adenosyl-L-methionine Kratky plot
Sample: SAM riboswitch element A aptamer domain (RNA) monomer, 39 kDa Listeria monocytogenes RNA
S-adenosyl-L-methionine monomer, 0 kDa
Buffer: 50 mM potassium phosphate, 135 mM NaCl, 5 mM MgCl2, pH: 6.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 9
Functional Validation of SAM Riboswitch Element A from Listeria monocytogenes. Biochemistry (2024)
Hall I, Zablock K, Sobetski R, Weidmann CA, Keane SC
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 56 nm3

SASDRS7 – ...S-adenosyl-L-methionine)

Putative peptide biosynthesis protein YydG experimental SAS data
DADIMODO model
Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 29
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat Chem Biol (2023)
Kubiak X, Polsinelli I, Chavas LMG, Fyfe CD, Guillot A, Fradale L, Brewee C, Grimaldi S, Gerbaud G, Thureau A, Legrand P, Berteau O, Benjdia A
RgGuinier 2.9 nm
Dmax 11.8 nm
VolumePorod 115 nm3

SASDRR7 – ...S-adenosyl-L-methionine) and a peptide subtrate

Putative peptide biosynthesis protein YydG experimental SAS data
DADIMODO model
Sample: Putative peptide biosynthesis protein YydG dimer, 80 kDa Bacillus subtilis (strain … protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Nov 30
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat Chem Biol (2023)
Kubiak X, Polsinelli I, Chavas LMG, Fyfe CD, Guillot A, Fradale L, Brewee C, Grimaldi S, Gerbaud G, Thureau A, Legrand P, Berteau O, Benjdia A
RgGuinier 2.9 nm
Dmax 12.0 nm
VolumePorod 113 nm3