Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Escherichia coli

SASDKL9 – Lymphostatin pH7.5

Sample:	Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

Activity of lymphostatin, a lymphocyte inhibitory virulence factor of pathogenic Escherichia coli, is dependent on a cysteine protease motif. J Mol Biol :167200 (2021)
Bease AG, Blackburn EA, Chintoan-Uta C, Webb S, Cassady-Cain RL, Stevens MP

R_g^Guinier	5.9	nm
D_max	19.3	nm
Volume^Porod	559	nm³

SASDKM9 – Lymphostatin pH5.5

Sample:	Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.9	nm
D_max	19.7	nm
Volume^Porod	565	nm³

SASDKN9 – Lymphostatin C1480A mutant pH 7.5

Efa1/LifA protein (C1480A mutant) experimental SAS data

Sample:	Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.9	nm
D_max	19.1	nm
Volume^Porod	557	nm³

SASDKP9 – Lymphostatin C1480A mutant pH5.5

Sample:	Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein
Buffer:	50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

R_g^Guinier	5.8	nm
D_max	19.3	nm
Volume^Porod	560	nm³

SASDL75 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Tetramer analyzed by SVD-EFA SEC-SAXS

Survival motor neuron proteinMaltose/maltodextrin-binding periplasmic protein experimental SAS data

Survival motor neuron protein Maltose/maltodextrin-binding periplasmic protein Kratky plot

Sample:	Survival motor neuron protein tetramer, 15 kDa Drosophila melanogaster protein Maltose/maltodextrin-binding periplasmic protein tetramer, 174 kDa Escherichia coli (strain … protein
Buffer:	20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19

Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
Gupta K, Wen Y, Ninan NS, Raimer AC, Sharp R, Spring AM, Sarachan KL, Johnson MC, Van Duyne GD, Matera AG

R_g^Guinier	3.9	nm
D_max	11.3	nm
Volume^Porod	340	nm³

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic proteinSurvival motor neuron protein experimental SAS data

Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot

Sample:	Maltose/maltodextrin-binding periplasmic protein octamer, 347 kDa Escherichia coli (strain … protein Survival motor neuron protein octamer, 31 kDa Drosophila melanogaster protein
Buffer:	20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 19

R_g^Guinier	5.6	nm
D_max	17.9	nm
Volume^Porod	800	nm³

SASDHW7 – RnlA-RnlB toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data

mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot

Sample:	MRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein Antitoxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	161	nm³

SASDHX7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data

mRNA endoribonuclease toxin LS Kratky plot

Sample:	MRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	10.7	nm
Volume^Porod	134	nm³

SASDHY7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot

Sample:	MRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.7	nm
D_max	20.1	nm
Volume^Porod	133	nm³

SASDHZ7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot

Sample:	MRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

R_g^Guinier	3.4	nm
D_max	12.7	nm
Volume^Porod	124	nm³

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