Browse by ORGANISM: Escherichia coli

SASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data
MULTIFOXS model
Sample: NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jun 27
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 88 nm3

SASDKS9 – mRNA endoribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data
MULTIFOXS model
Sample: MRNA endoribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R
RgGuinier 4.3 nm
Dmax 15.9 nm
VolumePorod 183 nm3

SASDKH6 – Sulfite reductase flavoprotein-43/hemoprotein heterodimer

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 42 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 3.2 nm
Dmax 9.9 nm

SASDKJ6 – Sulfite reductase flavoprotein-60/hemoprotein heterodimer

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein)Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 3.7 nm
Dmax 13.4 nm

SASDKK6 – Deuterated sulfite reductase hemoprotein

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 May 28
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 2.6 nm
Dmax 7.5 nm

SASDKL6 – Sulfite reductase flavoprotein-60 reduced with 10 molar equivalents sodium dithionite

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2018 Jul 11
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 3.4 nm
Dmax 12.2 nm

SASDKM6 – Sulfite reductase flavoprotein-60 (contrast-matched)/deuterated-hemoprotein heterodimer

Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] hemoprotein beta-component (Assimilatory NADPH-dependent sulfite reductase hemoprotein) monomer, 64 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 28
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 2.4 nm
Dmax 6.6 nm

SASDKN6 – Sulfite reductase flavoprotein-60/deuterated-hemoprotein (contrast-matched) heterodimer

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) experimental SAS data
Sample: Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) monomer, 61 kDa Escherichia coli (strain … protein
Buffer: 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH: 7.8
Experiment: SANS data collected at EQ-SANS (BL-6), Spallation Neutron Source on 2020 Jun 26
Small-angle neutron scattering solution structures of NADPH-dependent sulfite reductase Journal of Structural Biology 213(2):107724 (2021)
Murray D, Weiss K, Stanley C, Nagy G, Stroupe M
RgGuinier 3.2 nm
Dmax 12.4 nm

SASDKF4 – DNA binding protein of starvation (DPS)

DNA protection during starvation protein experimental SAS data
DAMMIN model
Sample: DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 26
Spatial Organization of Dps and DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV
RgGuinier 3.9 nm
Dmax 14.0 nm
VolumePorod 314 nm3

SASDKY4 – DNA binding protein of starvation (no EDTA)

DNA protection during starvation protein experimental SAS data
DAMMIN model
Sample: DNA protection during starvation protein dodecamer, 225 kDa Escherichia coli protein
Buffer: 20мМ Tris-HCl, 100 мМ NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 19
Spatial Organization of Dps and DNA-Dps Complexes. J Mol Biol :166930 (2021)
Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV
RgGuinier 3.8 nm
Dmax 11.9 nm
VolumePorod 277 nm3