Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Escherichia coli

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	2.4	nm
D_max	13.0	nm
Volume^Porod	30	nm³

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	34	nm³

SASDS57 – Heterotrimeric membrane protein complex FtsB-FtsL-FtsQ

Cell division protein FtsBCell division protein FtsLCell division protein FtsQ experimental SAS data

Sample:	Cell division protein FtsB hexamer, 70 kDa Escherichia coli (strain … protein Cell division protein FtsL hexamer, 82 kDa Escherichia coli (strain … protein Cell division protein FtsQ hexamer, 189 kDa Escherichia coli (strain … protein
Buffer:	20 mM HEPES pH 7.5, 200 mM NaCl, 0.2% of Cymal-5, pH: 7.5
Experiment:	SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Sep 23

Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome Nature Communications 14(1) (2023)
Nguyen H, Chen X, Parada C, Luo A, Shih O, Jeng U, Huang C, Shih Y, Ma C

R_g^Guinier	5.3	nm
D_max	16.5	nm
Volume^Porod	421	nm³

SASDQ87 – Solution structure of colibactin ClbK

Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK experimental SAS data

Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK Kratky plot

Sample:	Colibactin hybrid non-ribosomal peptide synthetase/type I polyketide synthase ClbK dimer, 476 kDa Escherichia coli protein
Buffer:	50 mM Hepes pH 7.5, 200 mM NaCl, 5 mM DTT, 5 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Sep 14

Architecture of a PKS-NRPS hybrid megaenzyme involved in the biosynthesis of the genotoxin colibactin Structure (2023)
Bonhomme S, Contreras-Martel C, Dessen A, Macheboeuf P

R_g^Guinier	8.0	nm
D_max	30.0	nm
Volume^Porod	1340	nm³

SASDRV6 – Transcription regulator PaaR2

Phage repressor protein CI (C120S) experimental SAS data

Sample:	Phage repressor protein CI (C120S) octamer, 122 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

Regulation of the Escherichia coli paaR2-paaA2-ParD2 toxin-antitoxin system
Marusa Prolic Kalinsek

R_g^Guinier	5.0	nm
D_max	16.8	nm
Volume^Porod	428	nm³

SASDRW6 – YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933

Phage antirepressor protein Cro experimental SAS data

Sample:	Phage antirepressor protein Cro monomer, 12 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM sodium acetate, 150 mM NaCl, 1 mM TCEP, pH: 5.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Feb 5

Structural-function relationship of YdaS, a Cro-type repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7
Marusa Prolic Kalinsek

R_g^Guinier	2.1	nm
D_max	7.6	nm
Volume^Porod	19	nm³

SASDQ78 – YbiB-ObgE GTPase complex

Uncharacterized protein YbiBGTPase Obg experimental SAS data

Uncharacterized protein YbiB GTPase Obg Kratky plot

Sample:	Uncharacterized protein YbiB dimer, 75 kDa Escherichia coli (strain … protein GTPase Obg dimer, 91 kDa Escherichia coli (strain … protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 24

YbiB: a novel interactor of the GTPase ObgE. Nucleic Acids Res (2023)
Deckers B, Vercauteren S, De Kock V, Martin C, Lazar T, Herpels P, Dewachter L, Verstraeten N, Peeters E, Ballet S, Michiels J, Galicia C, Versées W

R_g^Guinier	4.7	nm
D_max	18.6	nm
Volume^Porod	291	nm³

SASDQB9 – YdaT_toxin domain-containing protein

YdaT_toxin domain-containing protein experimental SAS data

Sample:	YdaT_toxin domain-containing protein tetramer, 74 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
Prolič-Kalinšek M, Volkov A, Hadži S, Van Dyck J, Bervoets I, Charlier D, Loris R

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	130	nm³

SASDQC9 – Mutant YdaT_toxin domain-containing protein (L111N/F118R)

YdaT_toxin domain-containing protein (mutant: L111N, F118R) experimental SAS data

Sample:	YdaT_toxin domain-containing protein (mutant: L111N, F118R) monomer, 18 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2021 Apr 14

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	32	nm³

SASDQD9 – N-terminal domain of YdaT_toxin domain-containing protein

Sample:	YdaT_toxin domain-containing protein monomer, 13 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

R_g^Guinier	1.7	nm
D_max	5.7	nm
Volume^Porod	29	nm³

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