Small Angle Scattering Biological Data Bank SASBDB


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24 hits found for Durand

SASDCK2 – Glucose Isomerase - Streptomyces rubiginosus

Sample:	Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
...Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, Tainer JA, Vachette P,...

R_g^Guinier	3.3	nm
D_max	9.2	nm
Volume^Porod	229	nm³

SASDCQ2 – 4Ca2+-calmodulin - Xenopus laevis

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDCJ3 – Bovine Serum Albumin

Sample:	Serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

R_g^Guinier	2.8	nm
D_max	8.7	nm
Volume^Porod	101	nm³

SASDEJ3 – Truncated neutophil cytosol factor 1, p47phox [1-342]

Neutophil cytosol factor 1 experimental SAS data

Sample:	Neutophil cytosol factor 1 monomer, 40 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar, IBBMC on 2009 Oct 16

Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase. J Biol Chem (2019)
...Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	58	nm³

SASDEK3 – The neutrophil cytosol factor 1 (p47phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 1 experimental SAS data

Sample:	Neutrophil cytosol factor 1 monomer, 46 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2008 Apr 23

R_g^Guinier	3.2	nm
D_max	12.5	nm
Volume^Porod	77	nm³

SASDEL3 – The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase

Neutrophil cytosol factor 2 experimental SAS data

The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase Rg histogram

Sample:	Neutrophil cytosol factor 2 monomer, 61 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 8
Experiment:	SAXS data collected at D24, LURE on 2003 Apr 9

R_g^Guinier	4.3	nm
D_max	16.0	nm
Volume^Porod	113	nm³

SASDNX3 – Calcium-bound Calmodulin, including structural models

Calcium-bound Calmodulin, including structural models Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
...Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	33	nm³

SASDNY3 – Calcium-bound Calmodulin complexed with Calmidazolium

Calmodulin-1Calmidazolium experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Calmidazolium monomer, 1 kDa
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
...Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	1.7	nm
D_max	5.2	nm
Volume^Porod	30	nm³

SASDJ64 – Calcium bound Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
...Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.3	nm
Volume^Porod	27	nm³

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDGM5 – Holo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data

Sample:	RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
...Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A

R_g^Guinier	4.4	nm
D_max	15.5	nm
Volume^Porod	89	nm³

SASDGN5 – apo-RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Sample:	RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 May 31

R_g^Guinier	8.3	nm
D_max	33.0	nm

SASDGP5 – Vibrio cholerae DciA

Sample:	DciA monomer, 18 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 26

Study of the DnaB:DciA interplay reveals insights into the primary mode of loading of the bacterial replicative helicase. Nucleic Acids Res (2021)
...Durand D, Le Cam E, Walbott H, Possoz C, Quevillon-Cheruel S, Ferat JL

R_g^Guinier	2.7	nm
D_max	10.1	nm
Volume^Porod	26	nm³

SASDGQ5 – Vibrio cholerae hexameric DnaB helicase with bound ATP (VcDnaB.ATP)

Replicative DNA helicase (DnaB) experimental SAS data

Replicative DNA helicase (DnaB) Kratky plot

Sample:	Replicative DNA helicase (DnaB) hexamer, 317 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Oct 4

R_g^Guinier	4.6	nm
D_max	13.0	nm
Volume^Porod	585	nm³

SASDGR5 – The Vibrio cholerae DciA/DnaB helicase complex in the presence of bound ATP (VcDnaB.DciA.ATP)

DnaB helicase complexed with ATPDciA experimental SAS data

DnaB helicase complexed with ATP DciA Kratky plot

Sample:	DnaB helicase complexed with ATP hexamer, 317 kDa Vibrio cholerae serotype … protein DciA , 55 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris-HCl, 100 mM NaCl, 1 mM ATP, pH: 8.8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Oct 4

R_g^Guinier	5.0	nm
D_max	15.6	nm
Volume^Porod	670	nm³

SASDNW6 – AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida

Apoptosis inducing protein experimental SAS data

Sample:	Apoptosis inducing protein monomer, 57 kDa Photobacterium damselae subsp. … protein
Buffer:	50 mM Hepes, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Apr 13

Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Nat Commun 14(1):7431 (2023)
...Durand D, Benz R, do Vale A, Dos Santos NMS

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	76	nm³

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data

Sample:	Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S

R_g^Guinier	4.0	nm
D_max	16.0	nm
Volume^Porod	175	nm³

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data

Sample:	comcde , 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer:	50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Feb 11

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S

R_g^Guinier	3.4	nm
D_max	10.7	nm
Volume^Porod	122	nm³

SASDAC7 – Complex LytTR-comcde

Sample:	comcde , 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer:	50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jun 2

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...Durand D, Quevillon-Cheruel S

R_g^Guinier	3.6	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data

EKC/KEOPS complex subunit GON7 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 26

Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, ...

R_g^Guinier	3.1	nm
D_max	12.5	nm
Volume^Porod	46	nm³

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data

Sample:	EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 May 12

R_g^Guinier	3.1	nm
D_max	11.5	nm
Volume^Porod	91	nm³

SASDFM8 – GON7, the fifth subunit of human KEOPS, bound to the EKC/KEOPS complex subunit, LAGE3

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3 experimental SAS data

EKC/KEOPS complex subunit GON7 EKC/KEOPS complex subunit LAGE3 Kratky plot

Sample:	EKC/KEOPS complex subunit GON7 dimer, 25 kDa Homo sapiens protein EKC/KEOPS complex subunit LAGE3 dimer, 33 kDa Homo sapiens protein
Buffer:	20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Mar 19

R_g^Guinier	3.6	nm
D_max	15.5	nm
Volume^Porod	126	nm³

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
...Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDCL9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	78	nm³

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