Small Angle Scattering Biological Data Bank SASBDB


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33 hits found for Guillem Hernandez

SASDTE5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 26 µM

Sample:	Nucleoprotein dimer, 82 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	5.7	nm
D_max	23.0	nm
Volume^Porod	160	nm³

SASDTF5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 49 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	5.8	nm
D_max	24.0	nm
Volume^Porod	181	nm³

SASDTG5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 103 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	6.2	nm
D_max	25.0	nm
Volume^Porod	215	nm³

SASDTH5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 206 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	6.8	nm
D_max	28.0	nm
Volume^Porod	578	nm³

SASDTJ5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 299 µM

Sample:	Nucleoprotein hexamer, 245 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein (N1-365, residues 1-365) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	7.1	nm
D_max	29.0	nm
Volume^Porod	708	nm³

SASDTX5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 19 µM

Sample:	Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein Full Length (N1-419, residues 1-419) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	6.3	nm
D_max	26.0	nm
Volume^Porod	233	nm³

SASDTY5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 50 µM

Sample:	Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein Full Length (N1-419, residues 1-419) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	6.9	nm
D_max	28.5	nm
Volume^Porod	571	nm³

SASDTZ5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 103 µM

Sample:	Nucleoprotein hexamer, 281 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein Full Length (N1-419, residues 1-419) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	7.6	nm
D_max	32.0	nm
Volume^Porod	764	nm³

SASDT26 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 216 µM

Sample:	Nucleoprotein octamer, 375 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein Full Length (N1-419, residues 1-419) oligomerization seen by SAXS
Guillem Hernandez

R_g^Guinier	8.2	nm
D_max	33.0	nm
Volume^Porod	1028	nm³

SASDVS6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245): 1666.7 µM

Sample:	Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: IDL176-246
Guillem Hernandez

R_g^Guinier	4.5	nm
D_max	19.0	nm
Volume^Porod	117	nm³

SASDVT6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data

Nucleoprotein (L223P, L227P, L230P) Kratky plot

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: IDL176-246
Guillem Hernandez

R_g^Guinier	2.8	nm
D_max	12.0	nm
Volume^Porod	14	nm³

SASDVU6 – SARS-CoV-2 N-protein (N1-245; amino acids 1-245): 167 µM

Sample:	Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein variants: N1-246
Guillem Hernandez

R_g^Guinier	5.2	nm
D_max	20.5	nm
Volume^Porod	121	nm³

SASDLC7 – Polyribonucleotide nucleotidyltransferase (PNPase): apo form

Polyribonucleotide nucleotidyltransferase experimental SAS data

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 246 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris-HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity. Biochimie (2023)
...Hernandez G, Fonseca L, Casinhas J, Cordeiro TN, Archer M, Arraiano CM, Brito JA, Matos RG

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	310	nm³

SASDLD7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-AMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	315	nm³

SASDLE7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-GMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	312	nm³

SASDJ28 – Ferric Iron Reductase - FhuF∆1-17

Ferric iron reductase protein FhuF (∆1-17) experimental SAS data

Sample:	Ferric iron reductase protein FhuF (∆1-17) monomer, 28 kDa Escherichia coli (strain … protein
Buffer:	20 mM Phosphate, 200 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Apr 16

Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli JBIC Journal of Biological Inorganic Chemistry (2021)
...Hernandez G, Lebègue E, Barrière F, Cordeiro T, Piccioli M, Louro R

R_g^Guinier	2.1	nm
D_max	8.8	nm
Volume^Porod	60	nm³

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM

Sample:	Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	19	nm³

SASDKF8 – N-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

Translocated intimin receptor Tir experimental SAS data

Sample:	Translocated intimin receptor Tir dimer, 50 kDa Escherichia coli O127:H6 … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Feb 18

The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Commun Biol 7(1):179 (2024)
...Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	46	nm³

SASDT22 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 476 µM

Sample:	Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	3.7	nm
D_max	16.0	nm
Volume^Porod	33	nm³

SASDT32 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 952 µM

Sample:	Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	4.1	nm
D_max	18.0	nm
Volume^Porod	53	nm³

SASDRQ5 – Small beta-barrel domain in the N-terminal region of the Translocated Intimin Receptor (Tir76-180)

Translocated intimin receptor Tir Kratky plot

Sample:	Translocated intimin receptor Tir monomer, 12 kDa Escherichia coli O127:H6 … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Nov 21

R_g^Guinier	1.7	nm
D_max	7.0	nm
Volume^Porod	21	nm³

SASDT42 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 238 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	2.4	nm
D_max	11.0	nm
Volume^Porod	10	nm³

SASDKH8 – C-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

Sample:	Translocated intimin receptor Tir monomer, 18 kDa Escherichia coli O127:H6 … protein
Buffer:	20 mM Sodium Phosphate, 150 mM NaCl, 1 mM EDTA, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Sep 15

R_g^Guinier	3.8	nm
D_max	12.8	nm
Volume^Porod	46	nm³

SASDT52 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	2.7	nm
D_max	11.0	nm
Volume^Porod	12	nm³

SASDT62 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 952 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	2.7	nm
D_max	12.0	nm
Volume^Porod	13	nm³

SASDSR9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 38.2 µM

Sample:	Nucleoprotein monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	3.3	nm
D_max	15.5	nm
Volume^Porod	56	nm³

SASDSS9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 76.4 µM

Sample:	Nucleoprotein dimer, 55 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	4.0	nm
D_max	18.0	nm
Volume^Porod	67	nm³

SASDST9 – SARS-CoV-2 N-protein (N1-245; residues 1-245): 294.7 µM

Sample:	Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	5.2	nm
D_max	22.0	nm
Volume^Porod	117	nm³

SASDSU9 – SARS-CoV-2 N-protein (N1-245, residues 1-245): 400 µM

Sample:	Nucleoprotein tetramer, 110 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	5.4	nm
D_max	24.0	nm
Volume^Porod	157	nm³

SASDSV9 – SARS-CoV-2 N-protein (N1-245; residues 1-245) L223P, L227P and L230P triple mutant: 36.4 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	3.2	nm
D_max	13.5	nm
Volume^Porod	53	nm³

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	3.5	nm
D_max	14.5	nm
Volume^Porod	47	nm³

SASDSX9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	4.4	nm
D_max	19.0	nm
Volume^Porod	68	nm³

SASDSY9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

SARS-CoV-2 N-protein variants: N1-246 and IDL176-246
Guillem Hernandez

R_g^Guinier	4.6	nm
D_max	19.0	nm
Volume^Porod	75	nm³

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