Small Angle Scattering Biological Data Bank SASBDB


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77 hits found for Kumar

SASDBS2 – Recombinant Tn antigen-binding lectin from Vatairea macrocarpa

Recombinant Tn antigen-binding lectin experimental SAS data

Sample:	Recombinant Tn antigen-binding lectin tetramer, 105 kDa Vatairea macrocarpa protein
Buffer:	100 mM sodium phosphate 150 mM NaCl 5% (v/v) glycerol, pH: 5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2014 Jan 11

Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: A new tool for cancer research. Int J Biochem Cell Biol 72:27-39 (2016)
...Kumar P, Graewert MA, Pereira RI, Cunha RMS, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS

R_g^Guinier	3.2	nm
D_max	9.5	nm
Volume^Porod	168	nm³

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Kumar Singh R, Volkov AN, Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1 experimental SAS data

Sample:	Macrophage mannose receptor 1 dimer, 315 kDa Mouse myeloma cell … protein
Buffer:	50mM Hepes, 100mM NaCl, 1mM DTT, pH: 7
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 15

Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med 12(530) (2020)
...Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M...

R_g^Guinier	7.9	nm
D_max	30.1	nm
Volume^Porod	584	nm³

SASDV75 – S9 carboxypeptidase from Bacillus subtilis (4 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data

Sample:	Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer:	10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18

Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
...Kumar A, Bhange GN, Kumar A, Makde RD

R_g^Guinier	5.2	nm
D_max	15.9	nm
Volume^Porod	439	nm³

SASDV85 – S9 carboxypeptidase from Bacillus subtilis (6 mg/ml)

Sample:	Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer:	10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18

Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
...Kumar A, Bhange GN, Kumar A, Makde RD

R_g^Guinier	5.2	nm
D_max	14.7	nm
Volume^Porod	435	nm³

SASDJ95 – E3 ubiquitin-protein ligase BRE1 complexed with E2 ubiquitin conjugating enzyme RAD6

E3 ubiquitin-protein ligase BRE1Ubiquitin-conjugating enzyme E2 2 experimental SAS data

Sample:	E3 ubiquitin-protein ligase BRE1 dimer, 50 kDa Saccharomyces cerevisiae (strain … protein Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11

Structural basis for the role of C-terminus acidic tail of Saccharomyces cerevisiae ubiquitin-conjugating enzyme (Rad6) in E3 ligase (Bre1) mediated recognition of histones International Journal of Biological Macromolecules :127717 (2023)
...Kumar P

R_g^Guinier	4.1	nm
D_max	10.4	nm
Volume^Porod	105	nm³

SASDV95 – S9 carboxypeptidase from Bacillus subtilis (8 mg/ml)

Sample:	Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer:	10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18

Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
...Kumar A, Bhange GN, Kumar A, Makde RD

R_g^Guinier	5.2	nm
D_max	15.3	nm
Volume^Porod	446	nm³

SASDJA5 – E2 ubiquitin conjugating enzyme RAD6

Ubiquitin-conjugating enzyme E2 2 experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 2 monomer, 20 kDa Saccharomyces cerevisiae (strain … protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Feb 11

R_g^Guinier	2.3	nm
D_max	6.6	nm
Volume^Porod	31	nm³

SASDFE6 – Sliding beta clamp

Beta sliding clamp experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

Regulation of futile ligation during early steps of BER in M. tuberculosis is carried out by a β-clamp-XthA-LigA tri-component complex International Journal of Biological Macromolecules (2022)
...Kumar N, Ali F, Kumar S, Jahan F, Ramachandran R

R_g^Guinier	3.7	nm
D_max	10.3	nm
Volume^Porod	186	nm³

SASDFF6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp complex

Beta sliding clampProbable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA (Exonuclease III) (EXO III) (AP endonuclease VI) monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 15

R_g^Guinier	3.5	nm
D_max	11.3	nm
Volume^Porod	98	nm³

SASDFG6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex

Beta sliding clampProbable exodeoxyribonuclease III protein XthADNA ligase A experimental SAS data

Sample:	Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	25.3	nm
Volume^Porod	501	nm³

SASDFH6 – Probable exodeoxyribonuclease III protein XthA, sliding beta clamp, DNA ligase-A tricomplex in the presence of DNA substrate

Probable exodeoxyribonuclease III protein XthADNA ligase ABeta sliding clampDNA ligase A nicked DNA substrate experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 31 kDa Mycobacterium tuberculosis protein DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Beta sliding clamp dimer, 86 kDa Mycobacterium tuberculosis protein DNA ligase A nicked DNA substrate dimer, 16 kDa DNA
Buffer:	50 mM Tris pH 8.0, 200 mM NaCl , 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 17

R_g^Guinier	5.8	nm
D_max	19.1	nm
Volume^Porod	496	nm³

SASDF87 – Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O

Binary toxin receptor Cqm1 protein experimental SAS data

Sample:	Binary toxin receptor Cqm1 protein dimer, 129 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
...Kumar V, Chidambaram R

R_g^Guinier	2.8	nm
D_max	9.7	nm

SASDF97 – Complex of Binary toxin receptor (Cqm1) with deuterated BinB component protein in 100% D2O

Binary toxin receptor Cqm1 proteinDeuterated BinB component of mosquito-larvicidal Binary toxin experimental SAS data

Sample:	Binary toxin receptor Cqm1 protein monomer, 65 kDa synthetic construct protein Deuterated BinB component of mosquito-larvicidal Binary toxin monomer, 56 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 23

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
...Kumar V, Chidambaram R

R_g^Guinier	1.9	nm
D_max	7.3	nm

SASDFA7 – Receptor binding BinB protein of mosquito-larvicidal Binary toxin in 100% D2O

Lysinibacillus Mosquito-larvicidal receptor binding component experimental SAS data

Sample:	Lysinibacillus Mosquito-larvicidal receptor binding component monomer, 53 kDa synthetic construct protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 24

Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization IUCrJ 7(2) (2020)
...Kumar V, Chidambaram R

R_g^Guinier	3.3	nm
D_max	9.9	nm

SASDMK7 – HomA outer membrane protein

HomA outer membrane protein experimental SAS data

Sample:	HomA outer membrane protein dimer, 146 kDa Helicobacter pylori protein
Buffer:	20 mM Tris-Cl, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2021 Feb 2

Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori. Sci Rep 11(1):24471 (2021)
...Kumar A, Makde RD, Ashish, Kodgire P

R_g^Guinier	8.3	nm
D_max	28.2	nm
Volume^Porod	426	nm³

SASDML7 – HomB outer membrane protein

HomB outer membrane protein experimental SAS data

Sample:	HomB outer membrane protein dimer, 148 kDa Helicobacter pylori protein
Buffer:	20 mM Tris-Cl, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2021 Feb 2

Biophysical characterization of the homodimers of HomA and HomB, outer membrane proteins of Helicobacter pylori. Sci Rep 11(1):24471 (2021)
...Kumar A, Makde RD, Ashish, Kodgire P

R_g^Guinier	8.2	nm
D_max	27.1	nm
Volume^Porod	500	nm³

SASDKP7 – Probable transcriptional regulatory protein Lrp/AsnC protein Rv2324

Probable transcriptional regulatory protein (Probably AsnC-family) experimental SAS data

Sample:	Probable transcriptional regulatory protein (Probably AsnC-family) dimer, 36 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Mar 1

Mycobacterium tuberculosis Rv2324 is a multifunctional feast/famine regulatory protein involved in growth, DNA replication and damage control. Int J Biol Macromol 252:126459 (2023)
...Kumar S, Jahan F, Krishnan MY, Ramachandran R

R_g^Guinier	2.7	nm
D_max	11.6	nm
Volume^Porod	156	nm³

SASDKQ7 – Probable transcriptional regulatory protein Lrp/AsnC protein Rv2324 in the presence of methionine

Sample:	Probable transcriptional regulatory protein (Probably AsnC-family) octamer, 143 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM EDTA, 2 mM methionine, pH: 7.5
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 5

R_g^Guinier	4.0	nm
D_max	13.8	nm
Volume^Porod	246	nm³

SASDTW7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A; SEC-SAXS, July 2022)

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) experimental SAS data

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) Kratky plot

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 7

Thermodynamic coupling of the tandem RRM domains of hnRNP A1 underlie its pleiotropic RNA binding functions. Sci Adv 10(28):eadk6580 (2024)
...Kumar A, Zhou Q, Wang Y, Hansen AL, Kutluay S, Roche J, Tolbert BS

R_g^Guinier	2.9	nm
D_max	12.0	nm
Volume^Porod	54	nm³

SASDTX7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A; SEC-SAXS, Sept 2022)

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Sep 27

R_g^Guinier	2.9	nm
D_max	12.0	nm
Volume^Porod	53	nm³

SASDTY7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 0.1 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

2.9

SASDTZ7 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 50 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.0

SASDT28 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 100 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.0

SASDT38 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 150 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.3

SASDT48 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 200 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.2

SASDT58 – Heterogeneous nuclear ribonucleoprotein A1-A (hnRNP A1-A) at 250 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/C175S) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.3

SASDT68 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 0.1 MPa

Heterogeneous nuclear ribonucleoprotein A1 experimental SAS data

Heterogeneous nuclear ribonucleoprotein A1 Kratky plot

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.6	nm
D_max	13.0	nm
Volume^Porod	36	nm³

SASDT78 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 50 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.6	nm
D_max	11.0	nm
Volume^Porod	33	nm³

SASDT88 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 100 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.5	nm
D_max	10.0	nm
Volume^Porod	32	nm³

SASDT98 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 150 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	29	nm³

SASDTA8 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 200 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.4	nm
D_max	9.5	nm
Volume^Porod	28	nm³

SASDTB8 – UP1 domain of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 250 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	2.4	nm
D_max	9.5	nm
Volume^Porod	29	nm³

SASDTC8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut); SEC-SAXS, July 2022)

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) experimental SAS data

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) Kratky plot

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 7

R_g^Guinier	3.4	nm
D_max	14.0	nm
Volume^Porod	57	nm³

SASDTD8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut); SEC-SAXS, Sept 2022)

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Sep 27

R_g^Guinier	3.7	nm
D_max	14.0	nm
Volume^Porod	58	nm³

SASDTE8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 0.1 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	3.6	nm
D_max	20.0	nm
Volume^Porod	98	nm³

SASDTF8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 50 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	3.7	nm
D_max	23.0	nm
Volume^Porod	104	nm³

SASDTG8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 100 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	3.6	nm
D_max	23.0	nm
Volume^Porod	94	nm³

SASDTH8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 150 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier	3.6	nm
D_max	23.0	nm
Volume^Porod	109	nm³

SASDTJ8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 200 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.7

SASDTK8 – Heterogeneous nuclear ribonucleoprotein A1-A mutant (hnRNP A1-A(mut)) at 250 MPa

Sample:	Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 34 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.6

SASDTL8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 0.1 MPa

Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) experimental SAS data

Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) Kratky plot

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

2.8

SASDTM8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 50 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.0

SASDTN8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 100 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.2

SASDTP8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 150 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

2.9

SASDTQ8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 200 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.1

SASDHR8 – Mosquito-larvicidal binary toxin receptor (Cqm1) protein

Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data

Sample:	Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) dimer, 129 kDa Culex quinquefasciatus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
...Kumar A, Kumar V

R_g^Guinier	4.0	nm
D_max	9.2	nm
Volume^Porod	97	nm³

SASDTR8 – UP1 domain mutant of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) at 250 MPa

Sample:	Heterogeneous nuclear ribonucleoprotein A1 (C43S/R75D/R88D/C175S ) monomer, 25 kDa Homo sapiens protein
Buffer:	100 mM HEPES, 350 mM NaCl, 0.5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2023 Mar 1

R_g^Guinier

3.2

SASDHS8 – Mosquito-larvicidal BinB protein

Binary larvicide subunit BinB experimental SAS data

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
...Kumar A, Kumar V

R_g^Guinier	2.9	nm
D_max	9.7	nm
Volume^Porod	48	nm³

SASDLS8 – Complex of Mtb GntR and Aptamer 1 [Rv0792c and Rv0792c_1]

Complex of Rv0792c and Rv0792c_1 experimental SAS data

Sample:	Complex of Rv0792c and Rv0792c_1 monomer, 74 kDa
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31

Structural and Functional Characterization of Rv0792c from Mycobacterium tuberculosis: Identifying Small Molecule Inhibitor against HutC Protein. Microbiol Spectr :e0197322 (2022)
...Kumar A, Sharma D, Ashish, Sharma TK, Singh R

R_g^Guinier	3.6	nm
D_max	12.0	nm

SASDHT8 – Complex of mosquito-larvicidal BinB protein and the Mosquito-larvicidal binary toxin receptor (Cqm1)

Binary larvicide subunit BinBSynthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) experimental SAS data

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer:	PBS buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl), pH: 7.4
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2020 Feb 24

Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
...Kumar A, Kumar V

R_g^Guinier	4.6	nm
D_max	11.7	nm
Volume^Porod	290	nm³

SASDLT8 – Complex of Mtb GntR and Aptamer 2 [Rv0792c and Rv0792c_2]

Complex of Rv0792c and Rv0792c_2 experimental SAS data

Sample:	Complex of Rv0792c and Rv0792c_2 monomer, 46 kDa
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31

R_g^Guinier	3.1	nm
D_max	9.8	nm

SASDHU8 – SANS study of the complex between mosquito-larvicidal BinB protein and the mosquito-larvicidal binary toxin receptor (Cqm1)

Sample:	Binary larvicide subunit BinB monomer, 53 kDa Lysinibacillus sphaericus protein Synthetic construct (mutant, His-tagged): Mosquito-larvicidal BinAB toxin receptor protein (Neutral and basic amino acid transport protein rBAT) monomer, 65 kDa Culex quinquefasciatus protein
Buffer:	25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O, pH: 7.5
Experiment:	SANS data collected at SANS-I facility, Dhruva Reactor, Bhabha Atomic Research Centre on 2019 Apr 26

Liposome-Based Study Provides Insight into Cellular Internalization Mechanism of Mosquito-Larvicidal BinAB Toxin. J Membr Biol (2020)
...Kumar A, Kumar V

R_g^Guinier	3.7	nm
D_max	12.9	nm
Volume^Porod	174	nm³

SASDLU8 – Complex of Mtb GntR and Aptamer 5 [Rv0792c and Rv0792c_5]

Complex of GntR protein: Aptamer5 experimental SAS data

Sample:	Complex of GntR protein: Aptamer5 monomer, 78 kDa
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 31

R_g^Guinier	3.4	nm
D_max	12.0	nm

SASDLV8 – SS DNA Aptamer 5 which binds Mtb GntR Homolog [Rv0792c_5]

SSDNA Aptamer from SELEX against Mtb Rv0792c experimental SAS data

Sample:	SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 14 kDa unidentified DNA
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 21

R_g^Guinier	1.8	nm
D_max	7.1	nm

SASDLW8 – SS DNA Aptamer 2 which binds Mtb GntR Homolog [Rv0792c_2]

Sample:	SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20

R_g^Guinier	1.9	nm
D_max	7.6	nm

SASDLX8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1]

Sample:	SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20

R_g^Guinier	2.1	nm
D_max	8.6	nm

SASDSX8 – Solution scattering studies on 20 kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis (ISPC-12)

20 kDa accessory protein experimental SAS data

Sample:	20 kDa accessory protein dimer, 42 kDa Bacillus thuringiensis serovar … protein
Buffer:	10 mM Tris, 100 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2023 Aug 15

20-kDa accessory protein (P20) from Bacillus thuringiensis subsp. israelensis ISPC-12: Purification, characterization, solution scattering and structural analysis International Journal of Biological Macromolecules :127985 (2023)
...Kumar A, Hire R, Makde R

R_g^Guinier	3.0	nm
D_max	8.6	nm
Volume^Porod	67	nm³

SASDLY8 – Mycobacterium tuberculosis Rv0792c, a GntR homolog

Recombinant Mycobacterium tuberculosis H37Rv experimental SAS data

Sample:	Recombinant Mycobacterium tuberculosis H37Rv dimer, 65 kDa Mycobacterium tuberculosis H37Rv protein
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 2

R_g^Guinier	3.2	nm
D_max	12.0	nm

SASDSY8 – S9C peptidase from Geobacillus stearothermophilus

Acylamino-acid-releasing enzyme (I277L, V491A) experimental SAS data

Sample:	Acylamino-acid-releasing enzyme (I277L, V491A) tetramer, 308 kDa Geobacillus stearothermophilus protein
Buffer:	10 mM Tris, 100 mM NaCl, pH: 8
Experiment:	SAXS data collected at BL-18, INDUS-2 on 2023 Aug 11

Crystal structure and solution scattering of Geobacillus stearothermophilus S9 peptidase reveal structural adaptations for carboxypeptidase activity. FEBS Lett (2024)
...Kumar A, Yadav P, Kumar A, Makde RD

R_g^Guinier	5.3	nm
D_max	13.0	nm
Volume^Porod	424	nm³

SASDLZ8 – SS DNA Aptamer 1 which binds Mtb GntR Homolog [Rv0792c_1]

Sample:	SSDNA Aptamer from SELEX against Mtb Rv0792c monomer, 13 kDa unidentified DNA
Buffer:	25 mM HEPES Buffer; 400 mM NaCl,, pH: 7.2
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 20

R_g^Guinier	2.1	nm
D_max	8.6	nm

SASDKU9 – Thymidylate kinase (dTMP kinase) at pH 8.0

Sample:	dTMP kinase dimer, 57 kDa Brugia malayi protein
Buffer:	20 mM Tris, 150 mM NaCl, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Oct 7

Crystal Structure of the Brugia malayi Thymidylate Kinase-dTMP Complex and Small Angle X-ray Scattering Experiments Identifies Changes in the Dimeric Association Compared to the Human Homolog Crystallography Reports 68(7):1150-1158 (2024)
...Kumar S, Ramachandran R

R_g^Guinier	3.0	nm
D_max	8.4	nm
Volume^Porod	81	nm³

SASDEU5 – Adenylation domain of DNA ligase A

Sample:	DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Sep 27

Salt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are import... Acta Crystallographica Section D Structural Biology 77(6) (2021)
...Kumar N, Ramachandran R

R_g^Guinier	2.4	nm
D_max	8.8	nm
Volume^Porod	69	nm³

SASDHU5 – Heat shock protein 70 (PfHSP70-1)

Heat shock protein 70Peptide experimental SAS data

Sample:	Heat shock protein 70 monomer, 74 kDa Plasmodium falciparum protein Peptide monomer, 1 kDa synthetic construct protein
Buffer:	50mM Tris, 5mM MgCl2, 500mM KCl, 5mM Bme, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

Structural-functional diversity of malaria parasite's PfHSP70-1 and PfHSP40 chaperone pair gives an edge over human orthologs in chaperone-assisted protein folding. Biochem J 477(18):3625-3643 (2020)
...Kumari V, Prakash C, Nag P, Kumar LS, Sharma SK, Ramachandran R, Kumar N

R_g^Guinier	5.3	nm
D_max	16.6	nm
Volume^Porod	450	nm³

SASDDD8 – class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III

Probable exodeoxyribonuclease III protein XthA experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 32 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 12

M. tuberculosis class II apurinic/ apyrimidinic-endonuclease/3'-5' exonuclease (XthA) engages with NAD+-dependent DNA ligase A (LigA) to counter futile cleavage and ligation cycles in base excision re... Nucleic Acids Res (2020)
...Kumar S, Soyar H, Dolma K, Pasupuleti M, Srivastava KK, Ampapathi RS, Ramachandran R

R_g^Guinier	2.4	nm
D_max	7.3	nm
Volume^Porod	56	nm³

SASDHR5 – Heat shock protein 40, subfamily A (PfHSP40)

HSP40, subfamily A experimental SAS data

Sample:	HSP40, subfamily A dimer, 97 kDa Plasmodium falciparum protein
Buffer:	50 mM Tris, 5 mM MgCl2, 300 mM KCl, 1 mM β-mercaptoethanol, 5% glycerol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Oct 24

R_g^Guinier	4.3	nm
D_max	15.0	nm
Volume^Porod	339	nm³

SASDEC6 – Adenylation Domain of DNA ligase A with NAD+

Sample:	DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Oct 6

R_g^Guinier	2.4	nm
D_max	6.2	nm
Volume^Porod	94	nm³

SASDDN9 – Mycobacterium tuberculosis DNA Ligase A

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13

R_g^Guinier	5.2	nm
D_max	16.7	nm
Volume^Porod	870	nm³

SASDG65 – Adenylation domain of DNA ligase A (LigA)

Sample:	DNA ligase A monomer, 37 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl 200 mM NaCl 2mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 May 21

R_g^Guinier	2.7	nm
D_max	8.9	nm
Volume^Porod	68	nm³

SASDDV9 – Mycobacterium tuberculosis DNA LigaseA with Nicked DNA

DNA ligase ANicked DNA experimental SAS data

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Nicked DNA dimer, 16 kDa DNA
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13

R_g^Guinier	4.4	nm
D_max	14.8	nm
Volume^Porod	262	nm³

SASDEW3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA)

DNA ligase AProbable exodeoxyribonuclease III protein XthA experimental SAS data

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13

R_g^Guinier	6.2	nm
D_max	23.9	nm
Volume^Porod	662	nm³

SASDEX3 – DNA ligase A (MtbLigA) complexed with exodeoxyribonuclease III protein (MtbXthA) in presence of nicked ds DNA

DNA ligase AProbable exodeoxyribonuclease III protein XthANicked DNA experimental SAS data

Sample:	DNA ligase A monomer, 76 kDa Mycobacterium tuberculosis protein Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein Nicked DNA monomer, 8 kDa DNA
Buffer:	50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 May 13

R_g^Guinier	4.5	nm
D_max	17.1	nm
Volume^Porod	117	nm³

SASDDQ8 – The complex formed between the class II apurinic/apyrimidinic-endonuclease/3'-5' exonuclease III (XthA) bound to the BRCT domain from Mycobacterium tuberculosis DNA ligase

Probable exodeoxyribonuclease III protein XthAM. tb. LigA BRCT domain experimental SAS data

Sample:	Probable exodeoxyribonuclease III protein XthA monomer, 33 kDa Mycobacterium tuberculosis protein M. tb. LigA BRCT domain monomer, 15 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 9

R_g^Guinier	3.7	nm
D_max	18.5	nm
Volume^Porod	112	nm³

SASDD88 – The BRCT domain from Mycobacterium tuberculosis DNA ligase

M.tb. LigA BRCT domain (DNA ligase A) experimental SAS data

Sample:	M.tb. LigA BRCT domain (DNA ligase A) monomer, 13 kDa Mycobacterium tuberculosis protein
Buffer:	50 mM Tris-HCl 500 mM NaCl 5mM β-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Jun 2

R_g^Guinier	1.6	nm
D_max	3.7	nm
Volume^Porod	23	nm³

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