Small Angle Scattering Biological Data Bank SASBDB


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38 hits found for Loris

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	140	nm³

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Dec 4

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Loris R

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	160	nm³

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein 33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Loris R

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	150	nm³

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data

Sample:	Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2017 Mar 6

Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Loris R

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	140	nm³

SASDS76 – Antitoxin HigA-2

Sample:	Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Apr 6

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
...Loris R

R_g^Guinier	2.3	nm
D_max	9.6	nm
Volume^Porod	43	nm³

SASDS86 – Antitoxin HigA-2 in complex with a DNA operator sequence

Antitoxin HigA-2DNA operator experimental SAS data

Antitoxin HigA-2 DNA operator Kratky plot

Sample:	Antitoxin HigA-2 dimer, 23 kDa Vibrio cholerae serotype … protein DNA operator dimer, 20 kDa DNA
Buffer:	20 mM Tris pH 8, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Apr 6

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae. Nat Commun 15(1):3105 (2024)
...Loris R

R_g^Guinier	2.7	nm
D_max	9.6	nm
Volume^Porod	57	nm³

SASDND6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant H123R, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (H123R) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1 (H123R) dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Oct 8

Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
...Loris R, Ylikallio E, Tyynismaa H, Bartesaghi L, Ruskamo S, Kursula P

R_g^Guinier	3.1	nm
D_max	9.9	nm
Volume^Porod	107	nm³

SASDNE6 – Human ganglioside-induced differentiation-associated protein 1 (GDAP1) missense mutant R120W, construct GDAP1∆302-358

Ganglioside-induced differentiation-associated protein 1 (R120W) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1 (R120W) dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 1

Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio (2022)
...Loris R, Ylikallio E, Tyynismaa H, Bartesaghi L, Ruskamo S, Kursula P

R_g^Guinier	3.1	nm
D_max	10.0	nm
Volume^Porod	105	nm³

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Sample:	Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
...Loris R

R_g^Guinier	3.4	nm
D_max	13.9	nm
Volume^Porod	190	nm³

SASDJR7 – Mouse cysteine sulphinic acid decarboxylase (CSAD)

Cysteine sulfinic acid decarboxylase experimental SAS data

Sample:	Cysteine sulfinic acid decarboxylase dimer, 117 kDa Mus musculus protein
Buffer:	20 mM HEPES, 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Feb 4

Structure and substrate specificity determinants of the taurine biosynthetic enzyme cysteine sulphinic acid decarboxylase. J Struct Biol :107674 (2020)
...Loris R, Haavik J, Kursula P

R_g^Guinier	3.4	nm
D_max	15.0	nm
Volume^Porod	198	nm³

SASDE48 – Toxin GraT

Putative killer protein, Toxin GraT. experimental SAS data

Putative killer protein, Toxin GraT. Kratky plot

Sample:	Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2018 Sep 21

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
...Loris R

R_g^Guinier	1.5	nm
D_max	5.5	nm
Volume^Porod	18	nm³

SASDE58 – Complex of toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.antitoxin GraA, antidote of toxin GraTPutative killer protein, Toxin GraT experimental SAS data

Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot

Sample:	Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein Putative killer protein, Toxin GraT monomer, 11 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
...Loris R

R_g^Guinier	2.3	nm
D_max	7.9	nm
Volume^Porod	63	nm³

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data

GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot

Sample:	GraTA operator region monomer, 20 kDa DNA antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Dec 17

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
...Loris R

R_g^Guinier	2.9	nm
D_max	10.3	nm
Volume^Porod	94	nm³

SASDJR8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 21

Structure of the Complete Dimeric Human GDAP1 Core Domain Provides Insights into Ligand Binding and Clustering of Disease Mutations Frontiers in Molecular Biosciences 7 (2021)
...Loris R, Kursula P

R_g^Guinier	3.1	nm
D_max	101.6	nm
Volume^Porod	111	nm³

SASDJS8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 dimer, 68 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	3.1	nm
D_max	9.2	nm
Volume^Porod	112	nm³

SASDJT8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 21

R_g^Guinier	2.7	nm
D_max	92.3	nm
Volume^Porod	71	nm³

SASDJU8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆295-358 monomer, 34 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	2.5	nm
D_max	8.9	nm
Volume^Porod	63	nm³

SASDJV8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358 dimer, 70 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 28

R_g^Guinier	3.1	nm
D_max	9.9	nm
Volume^Porod	106	nm³

SASDJW8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆303-358, mutant Y29E/C88A monomer, 35 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 28

R_g^Guinier	2.5	nm
D_max	8.7	nm
Volume^Porod	59	nm³

SASDJX8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	3.5	nm
D_max	10.8	nm
Volume^Porod	130	nm³

SASDJY8 – Dimeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 dimer, 74 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	3.1	nm
D_max	9.6	nm
Volume^Porod	121	nm³

SASDJZ8 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	2.7	nm
D_max	9.0	nm
Volume^Porod	69	nm³

SASDJ29 – Monomeric human ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 with hexadecanedioic acid

Sample:	Ganglioside-induced differentiation-associated protein 1, construct GDAP1∆319-358 monomer, 37 kDa Homo sapiens protein
Buffer:	25 mM HEPES, 300 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Jul 11

R_g^Guinier	2.5	nm
D_max	8.6	nm
Volume^Porod	65	nm³

SASDJ39 – Monomeric human ganglioside-induced differentiation-associated protein 1-like 1, GDAP1L1

Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) experimental SAS data

Sample:	Ganglioside-induced differentiation-associated protein 1-like 1 (∆125-143 isoform) monomer, 44 kDa Homo sapiens protein
Buffer:	20 mM TRIS pH 7.5, 150 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Dec 10

R_g^Guinier	2.7	nm
D_max	10.0	nm
Volume^Porod	73	nm³

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data

Sample:	Toxin CcdB dimer, 23 kDa Escherichia coli protein Antitoxin CcdA dimer, 17 kDa Escherichia coli protein Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer:	10 mM Tris 50 mM NaCl, pH: 7.3
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jul 24

Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
...Loris R, Lah J

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	92	nm³

SASDQB9 – YdaT_toxin domain-containing protein

YdaT_toxin domain-containing protein experimental SAS data

Sample:	YdaT_toxin domain-containing protein tetramer, 74 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
...Loris R

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	130	nm³

SASDQC9 – Mutant YdaT_toxin domain-containing protein (L111N/F118R)

YdaT_toxin domain-containing protein (mutant: L111N, F118R) experimental SAS data

Sample:	YdaT_toxin domain-containing protein (mutant: L111N, F118R) monomer, 18 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2021 Apr 14

R_g^Guinier	2.4	nm
D_max	8.2	nm
Volume^Porod	32	nm³

SASDQD9 – N-terminal domain of YdaT_toxin domain-containing protein

Sample:	YdaT_toxin domain-containing protein monomer, 13 kDa Escherichia coli O157:H7 protein
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Apr 16

R_g^Guinier	1.7	nm
D_max	5.7	nm
Volume^Porod	29	nm³

SASDQE9 – YdaT_toxin domain-containing protein bound to 30 base pair dsDNA

YdaT_toxin domain-containing proteinOm 30 base pair dsDNA experimental SAS data

Sample:	YdaT_toxin domain-containing protein tetramer, 74 kDa Escherichia coli O157:H7 protein Om 30 base pair dsDNA dimer, 37 kDa DNA
Buffer:	20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 14

R_g^Guinier	4.2	nm
D_max	12.8	nm
Volume^Porod	190	nm³

SASDHW7 – RnlA-RnlB toxin-antitoxin complex (mRNA endoribonuclease toxin LS : Antitoxin RnlB)

mRNA endoribonuclease toxin LSAntitoxin RnlB (C-terminal His-tagged) experimental SAS data

mRNA endoribonuclease toxin LS Antitoxin RnlB (C-terminal His-tagged) Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein Antitoxin RnlB (C-terminal His-tagged) monomer, 15 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	3.5	nm
D_max	10.0	nm
Volume^Porod	161	nm³

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data

Sample:	Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein Uncharacterized protein (Antitoxin) 16-mer, 135 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Dec 9

A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
...Loris R

R_g^Guinier	3.8	nm
D_max	16.2	nm
Volume^Porod	312	nm³

SASDHX7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS)

mRNA endoribonuclease toxin LS experimental SAS data

mRNA endoribonuclease toxin LS Kratky plot

Sample:	mRNA endoribonuclease toxin LS dimer, 80 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	3.7	nm
D_max	10.7	nm
Volume^Porod	134	nm³

SASDAB9 – EcPaaA2-HisEcParE2 construct

Sample:	Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein Uncharacterized protein (Antitoxin) octamer, 68 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Feb 5

A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
...Loris R

R_g^Guinier	3.3	nm
D_max	15.3	nm
Volume^Porod	166	nm³

SASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)

NRD-HEPN truncated variant of RnlA endoribonuclease experimental SAS data

Sample:	NRD-HEPN truncated variant of RnlA endoribonuclease dimer, 62 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jun 27

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	3.0	nm
D_max	10.4	nm
Volume^Porod	88	nm³

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein experimental SAS data

parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram

Sample:	Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Feb 5

A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
...Loris R

R_g^Guinier	2.2	nm
D_max	9.3	nm
Volume^Porod	36	nm³

SASDHY7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R255A single alanine mutant

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R255A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	3.7	nm
D_max	20.1	nm
Volume^Porod	133	nm³

SASDKS9 – mRNA endoribonuclease toxin LS (D245R mutant); batch-SAXS measurements in low salt buffer

mRNA endoribonuclease toxin LS (D245R mutant) experimental SAS data

Sample:	mRNA endoribonuclease toxin LS (D245R mutant) dimer, 84 kDa Escherichia coli (strain … protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Jul 18

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	4.3	nm
D_max	15.9	nm
Volume^Porod	183	nm³

SASDHZ7 – Escherichia coli RnlA (mRNA endoribonuclease toxin LS) - R318A single alanine mutant

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged experimental SAS data

mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged Kratky plot

Sample:	mRNA endoribonuclease toxin LS - R318A mutant; N-terminal His-tagged dimer, 84 kDa Escherichia coli protein
Buffer:	20 mM Tris, 150 mM NaCl, 1 mM TCEP, 5% glycerol, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 25

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA Nucleic Acids Research 49(12):7164-7178 (2021)
...Loris R

R_g^Guinier	3.4	nm
D_max	12.7	nm
Volume^Porod	124	nm³

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