Small Angle Scattering Biological Data Bank SASBDB


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143 hits found for Lucas

SASDHE2 – Type 1 insulin-like growth factor receptor ectodomains (IGF-1RΔβ)

Insulin-like growth factor 1 receptor experimental SAS data

Sample:	Insulin-like growth factor 1 receptor dimer, 202 kDa Homo sapiens protein
Buffer:	30 mM Tris, 140 mM NaCl, 0.02% w/v sodium azide,, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2008 Apr 22

Solution structure of ectodomains of the insulin receptor family: the ectodomain of the type 1 insulin-like growth factor receptor displays asymmetry of ligand binding accompanied by limited conformat... J Mol Biol 394(5):878-92 (2009)
Whitten AE, Smith BJ, Menting JG, Margetts MB, McKern NM, Lovrecz GO, Adams TE, Richards K, Bentley JD, Trewhella J, Ward CW, Lawrence MC

R_g^Guinier	5.1	nm
D_max	16.0	nm
Volume^Porod	357	nm³

SASDHF2 – Insulin receptor ectodomains (IRΔβ)

Sample:	Insulin receptor dimer, 203 kDa Homo sapiens protein
Buffer:	30 mM Tris, 140 mM NaCl, 0.02% w/v sodium azide,, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2008 Dec 7

R_g^Guinier	5.5	nm
D_max	17.0	nm
Volume^Porod	385	nm³

SASDKR3 – Deuterated calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein calcium ions tetramer, 0 kDa Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer:	50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	55	nm³

SASDP34 – Ubiquitin-conjugating enzyme E2 conjugated to deuterated Ubiquitin (SANS data at 0% v/v D2O, plus additional SANS with contrast variation and SAXS datasets)

Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S)Ubiquitin experimental SAS data

Sample:	Ubiquitin-conjugating enzyme E2 D1 (S22R, C85K, D87S) monomer, 17 kDa Homo sapiens protein Ubiquitin monomer, 9 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SANS data collected at Quokka - Small Angle Neutron Scattering, Australian Centre for Neutron Scattering (ANSTO) on 2019 May 24

Production and characterisation of modularly deuterated UBE2D1–Ub conjugate by small angle neutron and X-ray scattering European Biophysics Journal (2022)
Pietras Z, Duff A, Morad V, Wood K, Jeffries C, Sunnerhagen M

R_g^Guinier	2.1	nm
D_max	7.4	nm

SASDVZ5 – Human DNA repair protein RAD52 homolog (amino acids 1-212)

DNA repair protein RAD52 homolog experimental SAS data

Sample:	DNA repair protein RAD52 homolog decamer, 234 kDa Homo sapiens protein
Buffer:	20 mM Bis-Tris, 10% glycerol, 400 mM NaCl, 100 mM KCl, 1 mM EDTA, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS 1000, Eppley Structural Biology Facility, University of Nebraska Medical Center on 2014 Jul 25

A glimpse into the hidden world of the flexible C-terminal protein binding domains of human RAD52 Journal of Structural Biology 216(3):108115 (2024)
Struble L, Lovelace J, Borgstahl G

R_g^Guinier	4.1	nm
D_max	11.8	nm
Volume^Porod	375	nm³

SASDV26 – Human DNA repair protein RAD52 homolog (amino acids 1-303)

Sample:	DNA repair protein RAD52 homolog nonamer, 300 kDa Homo sapiens protein
Buffer:	20 mM Bis-Tris, 10% GLycerol, 400 mM NaCl, 100 mM KCl, 1mM EDTA, pH: 6
Experiment:	SAXS data collected at Rigaku BioSAXS 1000, Eppley Structural Biology Facility, University of Nebraska Medical Center on 2014 Jul 25

A glimpse into the hidden world of the flexible C-terminal protein binding domains of human RAD52 Journal of Structural Biology 216(3):108115 (2024)
Struble L, Lovelace J, Borgstahl G

R_g^Guinier	4.8	nm
D_max	18.3	nm
Volume^Porod	782	nm³

SASDL66 – High mannose neurofascin 155 immunoglobulin domains 1-6, 37.2 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B

R_g^Guinier	4.7	nm
D_max	18.0	nm
Volume^Porod	355	nm³

SASDL76 – High mannose neurofascin 155 immunoglobulin domains 1-6, 73.5 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	5.0	nm
D_max	21.0	nm
Volume^Porod	450	nm³

SASDL96 – High mannose neurofascin 155 immunoglobulin domains 1-6, 9.5 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.3	nm
D_max	17.0	nm
Volume^Porod	240	nm³

SASDLA6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 5.1 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.2	nm
D_max	17.0	nm
Volume^Porod	207	nm³

SASDLB6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 2.7 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.1	nm
D_max	17.0	nm
Volume^Porod	187	nm³

SASDLC6 – High mannose neurofascin 155 immunoglobulin domains 1-6, 1.3 μM

Sample:	Neurofascin dimer, 139 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	3.9	nm
D_max	12.6	nm
Volume^Porod	172	nm³

SASDLD6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 109.3 μM

Sample:	Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 25

R_g^Guinier	4.3	nm
D_max	18.0	nm
Volume^Porod	212	nm³

SASDLE6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 21.9 μM

Sample:	Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 25

R_g^Guinier	3.9	nm
D_max	16.0	nm
Volume^Porod	158	nm³

SASDLG6 – High mannose neurofascin 155 immunoglobulin domains 1-6 Thr216Ala, 1.1 μM

Sample:	Neurofascin T216A monomer, 70 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Jun 25

R_g^Guinier	3.6	nm
D_max	10.6	nm
Volume^Porod	139	nm³

SASDLH6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 24.6 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	5.0	nm
D_max	23.0	nm
Volume^Porod	180	nm³

SASDLJ6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 12.3 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.9	nm
D_max	23.0	nm
Volume^Porod	166	nm³

SASDLK6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 6.2 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.9	nm
D_max	23.0	nm
Volume^Porod	155	nm³

SASDLL6 – Complex glycan contactin 1 immunoglobulin domains 1-6, 3.1 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.9	nm
D_max	15.8	nm
Volume^Porod	118	nm³

SASDLM6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 21.8 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	5.2	nm
D_max	23.0	nm
Volume^Porod	154	nm³

SASDLN6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 10.9 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	5.0	nm
D_max	23.0	nm
Volume^Porod	150	nm³

SASDLP6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 5.5 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	5.0	nm
D_max	23.0	nm
Volume^Porod	146	nm³

SASDLQ6 – High mannose glycan contactin 1 immunoglobulin domains 1-6, 2.7 μM

Sample:	Contactin-1 I433V dimer, 134 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	4.9	nm
D_max	14.5	nm
Volume^Porod	100	nm³

SASDVQ6 – Retriever complex

VPS35 endosomal protein-sorting factor-likeVacuolar protein sorting-associated protein 29Vacuolar protein sorting-associated protein 26C experimental SAS data

Sample:	VPS35 endosomal protein-sorting factor-like monomer, 110 kDa Homo sapiens protein Vacuolar protein sorting-associated protein 29 monomer, 21 kDa Homo sapiens protein Vacuolar protein sorting-associated protein 26C monomer, 33 kDa Homo sapiens protein
Buffer:	50 mM Tris, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 Apr 28

Selective cargo and membrane recognition by SNX17 regulates its interaction with Retriever EMBO Reports (2024)
...Lucas M

R_g^Guinier	5.2	nm
D_max	21.5	nm
Volume^Porod	256	nm³

SASDLR6 – Complex glycan contactin 1 ectodomain, 20 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	8.1	nm
D_max	42.0	nm
Volume^Porod	520	nm³

SASDVR6 – Vacuolar protein sorting-associated protein 26C (VPS26C)

Vacuolar protein sorting-associated protein 26C experimental SAS data

Sample:	Vacuolar protein sorting-associated protein 26C monomer, 33 kDa Homo sapiens protein
Buffer:	50 mM Tris, 150 mM NaCl, 1 mM TCEP,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Apr 22

Selective cargo and membrane recognition by SNX17 regulates its interaction with Retriever EMBO Reports (2024)
...Lucas M

R_g^Guinier	2.7	nm
D_max	9.4	nm
Volume^Porod	44	nm³

SASDLS6 – Complex glycan contactin 1 ectodomain, 10 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	8.0	nm
D_max	43.0	nm
Volume^Porod	530	nm³

SASDLT6 – Complex glycan contactin 1 ectodomain, 5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	8.0	nm
D_max	43.0	nm
Volume^Porod	530	nm³

SASDLU6 – Complex glycan contactin 1 ectodomain, 2.5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	8.0	nm
D_max	25.7	nm
Volume^Porod	350	nm³

SASDLV6 – High mannose glycan contactin 1 ectodomain, 20.5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	7.0	nm
D_max	34.0	nm
Volume^Porod	285	nm³

SASDLW6 – High mannose glycan contactin 1 ectodomain, 11.1 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	27.0	nm
Volume^Porod	275	nm³

SASDLX6 – High mannose glycan contactin 1 ectodomain, 5.5 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	27.0	nm
Volume^Porod	270	nm³

SASDLY6 – High mannose glycan contactin 1 ectodomain, 2.4 μM

Sample:	Contactin-1 I433V dimer, 220 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 16

R_g^Guinier	6.8	nm
D_max	19.5	nm
Volume^Porod	254	nm³

SASDDD7 – Apolipoprotein E2

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Nov 29

The molecular basis for Apolipoprotein E4 as the major risk factor for late onset Alzheimer's disease. J Mol Biol (2019)
Raulin AC, Kraft L, Al-Hilaly YK, Xue WF, McGeehan JE, Atack JR, Serpell L

R_g^Guinier	5.6	nm
D_max	19.5	nm
Volume^Porod	400	nm³

SASDGD7 – Apolipoprotein E3 bound to 330 µg/mL heparin

Apolipoprotein E3Heparin experimental SAS data

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.3	nm
D_max	25.9	nm

SASDGE7 – Apolipoprotein E3 bound to 110 µg/mL heparin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.5	nm
D_max	25.8	nm

SASDGF7 – Apolipoprotein E3 bound to 37 µg/mL heparin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.9	nm
D_max	23.9	nm

SASDGG7 – Apolipoprotein E3 bound to 12 µg/mL heparin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.5	nm
D_max	22.0	nm

SASDGH7 – Apolipoprotein E3 bound to 4 µg/mL heparin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.3	nm
D_max	20.8	nm

SASDGJ7 – Apolipoprotein E4 bound to 330 µg/mL heparin

Apolipoprotein E4Heparin experimental SAS data

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.6	nm
D_max	26.5	nm

SASDGK7 – Apolipoprotein E4 bound to 110 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.6	nm
D_max	27.0	nm

SASDGL7 – Apolipoprotein E4 bound to 37 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	7.0	nm
D_max	25.8	nm

SASDGM7 – Apolipoprotein E4 bound to 12 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.7	nm
D_max	22.0	nm

SASDGN7 – Apolipoprotein E4 bound to 4 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.4	nm
D_max	21.2	nm

SASDGP7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (K143A K146A) mutant experimental SAS data

Heparin Apolipoprotein E4 (K143A K146A) mutant Kratky plot

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.8	nm

SASDGQ7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 110 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	21.2	nm

SASDGR7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 37 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	21.3	nm

SASDGS7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 12 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.5	nm

SASDGT7 – Mutant Apolipoprotein E4 (K143A K146A) bound to 4 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.5	nm

SASDGU7 – Truncated Apolipoprotein E4 (1-191) bound to 330 µg/mL heparin

HeparinApolipoprotein E4 (1-191) experimental SAS data

Heparin Apolipoprotein E4 (1-191) Kratky plot

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	3.1	nm
D_max	10.5	nm

SASDGV7 – Truncated Apolipoprotein E4 (1-191) bound to 37 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.6	nm
D_max	8.2	nm

SASDGW7 – Truncated Apolipoprotein E4 (1-191) bound to 12 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.5	nm
D_max	7.8	nm

SASDGX7 – Truncated Apolipoprotein E4 (1-191) bound to 4 µg/mL heparin

Sample:	Heparin monomer, 15 kDa Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.4	nm
D_max	8.0	nm

SASDGY7 – Apolipoprotein E4 (methylated) bound to 330 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.9	nm
D_max	23.7	nm

SASDGZ7 – Apolipoprotein E4 (methylated) bound to 110 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.8	nm
D_max	23.7	nm

SASDG28 – Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.4	nm
D_max	22.2	nm

SASDG38 – Apolipoprotein E4 (methylated) bound to 12 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.3	nm
D_max	20.8	nm

SASDG48 – Apolipoprotein E4 (methylated) bound to 4 µg/mL heparin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.2	nm

SASDG58 – Apolipoprotein E2 bound to 330 µM Suramin

Apolipoprotein E2Suramin experimental SAS data

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.2	nm
D_max	17.8	nm

SASDG68 – Apolipoprotein E2 bound to 110 µM Suramin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.6	nm
D_max	18.2	nm

SASDG78 – Apolipoprotein E2 bound to 37 µM Suramin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.9	nm
D_max	19.8	nm

SASDG88 – Apolipoprotein E2 bound to 12 µM Suramin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.3	nm

SASDD98 – Apolipoprotein E3

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Nov 29

R_g^Guinier	5.7	nm
D_max	19.5	nm
Volume^Porod	410	nm³

SASDG98 – Apolipoprotein E2 bound to 4 µM Suramin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.5	nm

SASDDA8 – Apolipoprotein E4

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Nov 29

R_g^Guinier	5.8	nm
D_max	19.6	nm
Volume^Porod	430	nm³

SASDGA8 – Apolipoprotein E3 bound to 330 µM Suramin

Apolipoprotein E3Suramin experimental SAS data

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.2	nm
D_max	18.6	nm

SASDGB8 – Apolipoprotein E3 bound to 110 µM Suramin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.6	nm
D_max	19.1	nm

SASDGC8 – Apolipoprotein E3 bound to 37 µM Suramin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	20.3	nm

SASDGD8 – Apolipoprotein E3 bound to 12 µM Suramin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.0	nm

SASDGE8 – Apolipoprotein E3 bound to 4 µM Suramin

Sample:	Apolipoprotein E3 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.1	nm

SASDGF8 – Apolipoprotein E4 bound to 330 µM Suramin

Apolipoprotein E4Suramin experimental SAS data

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.4	nm
D_max	19.6	nm

SASDGG8 – Apolipoprotein E4 bound to 110 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.5	nm
D_max	19.5	nm

SASDGH8 – Apolipoprotein E4 bound to 37 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	20.1	nm

SASDGJ8 – Apolipoprotein E4 bound to 12 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.1	nm

SASDGK8 – Apolipoprotein E4 bound to 4 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.4	nm

SASDGL8 – Apolipoprotein E4 (K143A K146A) mutant bound to 330 µM Suramin

Apolipoprotein E4 (K143A K146A) mutantSuramin experimental SAS data

Apolipoprotein E4 (K143A K146A) mutant Suramin Kratky plot

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	22.4	nm

SASDHL8 – Aquifex aeolicus McoA metaloxidase evolved variant (2F4)

McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) experimental SAS data

Sample:	McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) monomer, 55 kDa Aquifex aeolicus VF5 protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Sep 25

Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase ACS Catalysis :5022-5035 (2022)
...Lucas M, Frazão C, Monza E, Masgrau L, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	6.8	nm
Volume^Porod	78	nm³

SASDGM8 – Apolipoprotein E4 (K143A K146A) mutant bound to 110 µM Suramin

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.5	nm
D_max	18.9	nm

SASDHM8 – Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352) experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 18

R_g^Guinier	2.2	nm
D_max	6.6	nm
Volume^Porod	74	nm³

SASDGN8 – Apolipoprotein E4 (K143A K146A) mutant bound to 37 µM Suramin

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.9	nm
D_max	19.9	nm

SASDGP8 – Apolipoprotein E4 (K143A K146A) mutant bound to12 µM Suramin

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	20.2	nm

SASDGQ8 – Apolipoprotein E4 (K143A K146A) mutant bound to 4 µM Suramin

Sample:	Apolipoprotein E4 (K143A K146A) mutant tetramer, 138 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jul 21

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.1	nm
D_max	20.4	nm

SASDNQ8 – High mannose contactin 2 immunoglobulin domains1-6, 3μM

Sample:	Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.3	nm
D_max	20.0	nm
Volume^Porod	300	nm³

SASDGR8 – Apolipoprotein E4 (methylated) bound to 330 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	20.6	nm

SASDNR8 – High mannose contactin-2 immunoglobulin domains1-6, 6μM

Sample:	Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.4	nm
D_max	21.0	nm
Volume^Porod	325	nm³

SASDGS8 – Apolipoprotein E4 (methylated) bound to 110 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.7	nm
D_max	18.1	nm

SASDNS8 – High mannose contactin-2 immunoglobulin domains1-6, 13μM

Sample:	Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.5	nm
D_max	21.4	nm
Volume^Porod	350	nm³

SASDGT8 – Apolipoprotein E4 (methylated) bound to 37 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	5.8	nm
D_max	19.6	nm

SASDNT8 – High mannose contactin-2 immunoglobulin domains1-6, 26μM

Sample:	Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	6.0	nm
D_max	30.0	nm
Volume^Porod	380	nm³

SASDGU8 – Apolipoprotein E4 (methylated) bound to 12 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	19.4	nm

SASDNU8 – High mannose contactin-2 immunoglobulin domains1-6, 52μM

Sample:	Contactin-2 dimer, 150 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	6.1	nm
D_max	32.0	nm
Volume^Porod	408	nm³

SASDFV8 – Apolipoprotein E2 (ApoE2) bound to 4 µg/mL heparin

Apolipoprotein E2Heparin experimental SAS data

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.2	nm
D_max	20.7	nm
Volume^Porod	504	nm³

SASDGV8 – Apolipoprotein E4 (methylated) bound to 4 µM Suramin

Sample:	Apolipoprotein E4 tetramer, 139 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.0	nm
D_max	19.7	nm

SASDNV8 – Complex glycan contactin-2 immunoglobulin domains1-6, 1μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.6	nm
D_max	25.0	nm
Volume^Porod	230	nm³

SASDFW8 – Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.4	nm
D_max	21.7	nm
Volume^Porod	531	nm³

SASDGW8 – Truncated Apolipoprotein E4 (1-191) bound to 330 µM Suramin

Apolipoprotein E4 (1-191)Suramin experimental SAS data

Apolipoprotein E4 (1-191) Suramin Kratky plot

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	3.1	nm
D_max	11.1	nm

SASDNW8 – Complex glycan contactin-2 immunoglobulin domains1-6, 3μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.4	nm
D_max	25.0	nm
Volume^Porod	185	nm³

SASDFX8 – Apolipoprotein E2 (ApoE2) bound to 37 µg/mL heparin

Sample:	Apolipoprotein E2 tetramer, 139 kDa Homo sapiens protein Heparin monomer, 15 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	6.5	nm
D_max	23.4	nm
Volume^Porod	584	nm³

SASDGX8 – Truncated Apolipoprotein E4 (1-191) bound to 110 µM Suramin

Sample:	Apolipoprotein E4 (1-191) monomer, 23 kDa Homo sapiens protein Suramin monomer, 1 kDa
Buffer:	20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 May 3

Therapeutic approaches to ApoE University of Sussex PhD thesis 2019 (2019)
Lucas Kraft

R_g^Guinier	2.8	nm
D_max	9.5	nm

SASDNX8 – Complex glycan contactin-2 immunoglobulin domains1-6, 5μM

Sample:	Contactin-2 dimer, 157 kDa Mus musculus protein
Buffer:	25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Dec 17

Contactin 2 homophilic adhesion structure and conformational plasticity Structure (2023)
Chataigner L, Thärichen L, Beugelink J, Granneman J, Mokiem N, Snijder J, Förster F, Janssen B

R_g^Guinier	5.5	nm
D_max	22.0	nm
Volume^Porod	353	nm³

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