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18 hits found for Sterckx

SASDGS5 – MvaT (low salt data set)

MvaT(mutant) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: MvaT(mutant) dimer, 28 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Bis-Tris 50 mM KCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2018 May 11
Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res (2020)
...Sterckx YGJ, Volkov AN, Vreede J, Giachin G, van Schaik P, Ubbink M, Dame RT
RgGuinier 3.6 nm
Dmax 14.7 nm
VolumePorod 47 nm3

SASDGT5 – MvaT (high salt data set)

MvaT(mutant) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: MvaT(mutant) dimer, 28 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Bis-Tris 300 mM KCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2018 May 11
Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res (2020)
...Sterckx YGJ, Volkov AN, Vreede J, Giachin G, van Schaik P, Ubbink M, Dame RT
RgGuinier 3.8 nm
Dmax 15.8 nm
VolumePorod 50 nm3

SASDRF8 – Plasmodium falciparum circumsporozoite protein N-terminal domain at pH 6.6

Circumsporozoite protein experimental SAS data
Circumsporozoite protein Kratky plot
Sample: Circumsporozoite protein monomer, 11 kDa Plasmodium falciparum (isolate … protein
Buffer: 50 mM MES, 500 mM NaCl, 1 mM TCEP, pH: 6.6
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain Protein Science (2023)
...Sterckx Y
RgGuinier 2.3 nm
Dmax 9.1 nm
VolumePorod 24 nm3

SASDBA9 – E. coli CcdB2-CcdA2-CcdB2 toxin/antitoxin heterohexamer complex

Toxin CcdBAntitoxin CcdAToxin CcdB experimental SAS data
MODELLER model
Sample: Toxin CcdB dimer, 23 kDa Escherichia coli protein
Antitoxin CcdA dimer, 17 kDa Escherichia coli protein
Toxin CcdB dimer, 23 kDa Escherichia coli protein
Buffer: 10 mM Tris 50 mM NaCl, pH: 7.3
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jul 24
Molecular mechanism governing ratio-dependent transcription regulation in the ccdAB operon. Nucleic Acids Res 45(6):2937-2950 (2017)
...Sterckx YG, Welte T, De Greve H, Charlier D, Efremov R, Loris R, Lah J
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 92 nm3

SASDSP2 – Saccharomyces cerevisiae Mer2 coiled-coiled with N-terminal SUMO-tag

Recombination protein 107 experimental SAS data
Sample: Recombination protein 107 tetramer, 139 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 8.3 nm
Dmax 32.2 nm

SASDTA5 – Trypanosoma brucei gambiense variable surface glycoprotein LiTat 1.5

Variable surface glycoprotein LiTat 1.5 experimental SAS data
BILBOMD model
Sample: Variable surface glycoprotein LiTat 1.5 dimer, 98 kDa Trypanosoma brucei gambiense protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
...Sterckx YG, Zoll S
RgGuinier 4.4 nm
Dmax 17.2 nm
VolumePorod 170 nm3

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) 16-mer, 135 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 9
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.8 nm
Dmax 16.2 nm
VolumePorod 312 nm3

SASDSU2 – Mus musculus Iho1 coiled-coiled with N-terminal SUMO-tag

Interactor of HORMAD1 protein 1 (M198V, V230I) experimental SAS data
Sample: Interactor of HORMAD1 protein 1 (M198V, V230I) tetramer, 127 kDa Mus musculus protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 7.2 nm
Dmax 25.5 nm

SASDTB5 – Trypanosoma brucei gambiense variant surface glycoprotein 3.1

Variant surface glycoprotein 3.1 experimental SAS data
BILBOMD model
Sample: Variant surface glycoprotein 3.1 dimer, 103 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
...Sterckx YG, Zoll S
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 149 nm3

SASDAB9 – EcPaaA2-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE octamer, 102 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) octamer, 68 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 166 nm3

SASDST2 – Saccharomyces pombe Rec15 coiled-coiled with N-terminal SUMO-tag

Meiotic recombination protein rec15 experimental SAS data
3D-DART model
Sample: Meiotic recombination protein rec15 tetramer, 128 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 6.9 nm
Dmax 26.5 nm

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein experimental SAS data
parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram
Sample: Plasmid stabilization protein ParE monomer, 13 kDa Escherichia coli protein
Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 2.2 nm
Dmax 9.3 nm
VolumePorod 36 nm3

SASDT75 – Trypanosoma brucei gambiense invariant surface glycoprotein 43 (ISG43)

T. brucei spp.-specific protein experimental SAS data
BILBOMD model
Sample: T. brucei spp.-specific protein monomer, 38 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
...Sterckx YG, Zoll S
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 53 nm3

SASDSS2 – Arabidopsis thaliana PRD3 coiled-coiled with N-terminal SUMO-tag

Putative recombination initiation defects 3 experimental SAS data
OTHER model
Sample: Putative recombination initiation defects 3 tetramer, 129 kDa Arabidopsis thaliana protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 7.8 nm
Dmax 27.5 nm

SASDT85 – Trypanosoma brucei gambiense invariant surface glycoprotein 64 (ISG64)

64 kDa invariant surface glycoprotein, putative experimental SAS data
BILBOMD model
Sample: 64 kDa invariant surface glycoprotein, putative monomer, 40 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Sep 8
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
...Sterckx YG, Zoll S
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 56 nm3

SASDSR2 – Zea mays PAIR1 coiled-coiled with N-terminal SUMO-tag

Protein PAIR1 experimental SAS data
OTHER model
Sample: Protein PAIR1 tetramer, 132 kDa Zea mays protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 8.0 nm
Dmax 31.7 nm

SASDT95 – Trypanosoma brucei gambiense invariant surface glycoprotein 75 (ISG75)

ISG75 experimental SAS data
BILBOMD model
Sample: ISG75 monomer, 50 kDa Trypanosoma brucei gambiense protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Beyond the VSG Layer: Exploring the Role of Intrinsic Disorder in the Invariant Surface Glycoproteins of African Trypanosomes. PLoS Pathog 20(4):e1012186 (2024)
...Sterckx YG, Zoll S
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 85 nm3

SASDSQ2 – Sordaria macrospora ASY2 coiled-coiled with N-terminal SUMO-tag

WGS project CABT00000000 data, contig 2.12 experimental SAS data
OTHER model
Sample: WGS project CABT00000000 data, contig 2.12 tetramer, 155 kDa Sordaria macrospora (strain … protein
Buffer: 25 mM HEPES-NaOH, 500 mM NaCl, 5 mM EDTA, 5% glycerol, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 14
Evolutionary conservation of the structure and function of meiotic Rec114-Mei4 and Mer2 complexes. Genes Dev 37(11-12):535-553 (2023)
...Sterckx YG, Volkov AN, Claeys Bouuaert C
RgGuinier 9.6 nm
Dmax 38.0 nm