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39 hits found for Serum albumin

SASDF22 – ...serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: somapacitan monomer, 22 kDa Homo sapiens protein
...serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
...serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDA32 – ...serum albumin in HEPES

Bovine Serum Albumin experimental SAS data
DAMMIF model
Sample: ...Serum Albumin monomer, 69 kDa Bos taurus (Bovine) protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 12
Standard proteins
Darja Ruskule
RgGuinier 2.9 nm
Dmax 9.3 nm
VolumePorod 97 nm3

SASDF32 – ...serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: ...serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
...serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDF42 – ...serum albumin in complex with somapacitan

Human serum albuminSomapacitan experimental SAS data
SASREF model
Sample: ...serum albumin monomer, 66 kDa Homo sapiens protein
Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer: 100 mM MES, 140 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4
...serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 202 nm3

SASDPH3 – Benchmarking extraction methods of scattering patterns of poorly resolved components from a SEC-SAXS dataset of BSA.

Bovine Serum Albumin experimental SAS data
Bovine Serum Albumin Kratky plot
Sample: ...Serum Albumin trimer, 199 kDa Bos taurus protein
Buffer: 20 mM Hepes, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Sep 24
SEC-SAXS: Experimental set-up and software developments build up a powerful tool. Methods Enzymol 677:221-249 (2022)
Pérez J, Thureau A, Vachette P
RgGuinier 5.2 nm
Dmax 17.6 nm
VolumePorod 370 nm3

SASDBJ3 – ...serum albumin, monomer from SEC-SAXS

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 100 nm3

SASDCJ3 – ...Serum Albumin

Serum albumin experimental SAS data
DAMMIN model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...
RgGuinier 2.8 nm
Dmax 8.7 nm
VolumePorod 101 nm3

SASDBK3 – ...serum albumin, dimer from SEC-SAXS

Bovine serum albumin, dimer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin, dimer dimer, 133 kDa Bos taurus protein
Buffer: 25 mM Tris 150 mM NaCl 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 23
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments. Nat Protoc 11(11):2122-2153 (2016)
Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI
RgGuinier 3.9 nm
Dmax 12.7 nm
VolumePorod 202 nm3

SASDDN3 – ...serum albumin mixture: averaged and individual data frames (subtracted and unsubtracted test sets)

Bovine serum albumin experimental SAS data
Bovine serum albumin Kratky plot
Sample: ...serum albumin , 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 25
Machine Learning Methods for X-Ray Scattering Data Analysis from Biomacromolecular Solutions. Biophys J 114(11):2485-2492 (2018)
Franke D, Jeffries CM, Svergun DI
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 117 nm3

SASDBT4 – ...serum albumin monomer (measured by SEC-SAXS)

Bovine serum albumin, monomer experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin, monomer monomer, 66 kDa Bos taurus protein
Buffer: 20 mM Tris 142 mM NaCl 5 % Glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 22
...Serum Albumin measured by SEC-SAXS
Martha Brennich
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 118 nm3

SASDE65 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 92 nm3

SASDE75 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 104 nm3

SASDE85 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDE95 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.9 nm
Dmax 8.6 nm
VolumePorod 74 nm3

SASDEA5 – ...serum albumin monomer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 25
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 78 nm3

SASDEB5 – ...serum albumin from in-house SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 May 4
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 99 nm3

SASDEC5 – ...serum albumin dimer from in-house SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin dimer, 133 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 200 nm3

SASDED5 – ...serum albumin monomer from synchrotron SEC-SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS + 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 14
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 84 nm3

SASDEE5 – ...serum albumin from synchrotron SAXS

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDAA6 – ...serum albumin monomer and mixtures

Human serum albumin monomer experimental SAS data
DAMMIF model
Sample: ...serum albumin monomer monomer, 66 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 22
Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 103 nm3

SASDP67 – ...serum albumin at neutral pH, 4 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 99 nm3

SASDP77 – ...serum albumin at neutral pH, 4 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 94 nm3

SASDP87 – ...serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 3.0 nm
Dmax 9.5 nm
VolumePorod 108 nm3

SASDP97 – ...serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 69 kDa Bos taurus protein
Buffer: 25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment: SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7
Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 104 nm3

SASDCF8 – ...serum albumin (WAXS)

Serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Serum albumin monomer, 66 kDa Bos taurus protein
Buffer: Hepes, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 25
WAXS benchmark on standard proteins
Maxim Petoukhov
RgGuinier 2.7 nm

SASDRL8 – ...serum albumin

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7
NSRRC TPS13A standard protein archive
Orion Shih
RgGuinier 2.9 nm
Dmax 8.9 nm
VolumePorod 84 nm3

SASDCQ8 – ...serum albumin (BSA)

Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: TRIS 50mM, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.8 nm

SASDRQ8 – ...serum albumin monomer separated using asymmetric flow field flow fractionation (AFFFF)

Albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: 10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8
Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 104 nm3

SASDHV8 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.0

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 267 nm3

SASDHW8 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 5.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 5.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 21
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.0 nm
VolumePorod 274 nm3

SASDHX8 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 258 nm3

SASDQX8 – ...serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 14
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 3.1 nm

SASDHY8 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM histidine, pH 7.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM histidine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jun 26
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 5.0 nm
Dmax 17.4 nm
VolumePorod 270 nm3

SASDQY8 – ...serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

Albumin experimental SAS data
Albumin Kratky plot
Sample: Albumin monomer, 66 kDa Bos taurus protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SANS data collected at SPB/SFX, European XFEL on 2021 May 15
Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, K...
RgGuinier 2.7 nm

SASDHZ8 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM TRIS, pH 8.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM TRIS, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 15
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 239 nm3

SASDH29 – ...serum albumin/Neprilysin fusion protein (HSA-NEP): 10 mM phosphate, pH 6.5

Neprilysin - G400V mutantHuman serum albumin - C58S mutant experimental SAS data
EOM/RANCH model
Sample: Neprilysin - G400V mutant monomer, 80 kDa Homo sapiens protein
...serum albumin - C58S mutant monomer, 66 kDa Homo sapiens protein
Buffer: 10 mM phosphate, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 7
Albumin-neprilysin fusion protein: understanding stability using small angle X-ray scattering and molecular dynamic simulations. Sci Rep 10(1):10089 (2020)
Kulakova A, Indrakumar S, Sønderby Tuelung P, Mahapatra S, Streicher WW, Peters GHJ, Harris P
RgGuinier 4.9 nm
Dmax 16.7 nm
VolumePorod 240 nm3

SASDFQ8 – ...serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDFR8 – ...serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDF99 – ...serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: ...serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3