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39 hits found for Uncharacterized protein

SASDD52 – ...protein AmaxFRP.1-106

Uncharacterized fluorescence recovery protein experimental SAS data
GASBOR model
Sample: Uncharacterized fluorescence recovery protein dimer, 24 kDa Arthrospira maxima CS-328 protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDTA, 2 mM dithiothreitol, 3 % v/v glycerol, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP. Biochim Biophys Acta 1859(5):382-393 (2018)
Slonimskiy YB, Maksimov EG, Lukashev EP, Moldenhauer M, Jeffries CM, Svergun DI, Friedrich T, Sluchanko NN
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 35 nm3

SASDHW2 – The Legionella pneumophila type II secretion system substrate NttE

Uncharacterized protein experimental SAS data
GASBOR model
Sample: Uncharacterized protein dimer, 66 kDa Legionella pneumophila protein
Buffer: 20 mM Tris, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 18
Structure, Dynamics and Cellular Insight Into Novel Substrates of the Legionella pneumophila Type II Secretion System Frontiers in Molecular Biosciences 7 (2020)
Portlock T, Tyson J, Dantu S, Rehman S, White R, McIntire I, Sewell L, Richardson K, Shaw R, Pandini A, Cianciotto N, Garnett J
RgGuinier 2.9 nm
Dmax 9.4 nm
VolumePorod 114 nm3

SASDC63 – ...protein in complex with BaK BH3 peptide (FPV039:BAK)

Bcl-2-like protein FPV039Uncharacterized protein (BAK1) experimental SAS data
Bcl-2-like protein FPV039 Uncharacterized protein (BAK1) Kratky plot
Sample: ...protein FPV039 monomer, 17 kDa Fowlpox virus protein
Uncharacterized protein (BAK1) monomer, 3 kDa Gallus gallus protein
Buffer: 20 mM trisodium citrate pH, 200 mM NaCl, pH: 6
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Oct 2
...protein FPV039. J Biol Chem 292(22):9010-9021 (2017)
Anasir MI, Caria S, Skinner MA, Kvansakul M
RgGuinier 2.0 nm

SASDPC4 – ...protein

TIR domain-containing proteinUncharacterized protein (PIWI) experimental SAS data
ALPHAFOLD model
Sample: ...protein monomer, 57 kDa Rhodopseudomonas palustris (strain … protein
Uncharacterized protein (PIWI) monomer, 56 kDa Rhodopseudomonas palustris (strain … protein
Buffer: 20 mM Tris-HCl, pH8.0, 200 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Sep 27
...protein
Elena Manakova
RgGuinier 3.6 nm
Dmax 12.0 nm
VolumePorod 192 nm3

SASDBK4 – The 1:1:3:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F short Cascade complex

short-crRNA: CRISPR/Cas Type I-F Cascade componentCas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)Trimeric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822) experimental SAS data
DAMMIF model
Sample: short-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 14 kDa Shewanella putrefaciens RNA
...protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1821) trimer, 112 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
Buffer: 50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Jun 27
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
RgGuinier 4.1 nm
Dmax 14.2 nm

SASDBL4 – The 1:1:6:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F wild-type Cascade complex

Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822)Hexameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)wildtype-crRNA: CRISPR/Cas Type I-F Cascade component experimental SAS data
DAMMIF model
Sample: ...protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1821) hexamer, 223 kDa Shewanella putrefaciens protein
wildtype-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 19 kDa RNA
Buffer: 50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Jun 27
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
RgGuinier 5.4 nm
Dmax 18.4 nm

SASDBM4 – The 1:1:9:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F long Cascade complex

Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822)Nonameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)long-crRNA: CRISPR/Cas Type I-F Cascade component experimental SAS data
DAMMIF model
Sample: ...protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
...Uncharacterized protein, Sputcn32_1821) nonamer, 335 kDa Shewanella putrefaciens protein
long-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 25 kDa RNA
Buffer: 50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jan 30
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
RgGuinier 6.5 nm
Dmax 21.6 nm

SASDQN4 – Complex of CRISPR associated Lon protease (CalpL) with a 10 kDa C-terminal fragment of CalpT a putative MazF-like toxin (CalpT10)

SAVED domain-containing proteinUncharacterized protein (putative MazF-like toxin) experimental SAS data
CUSTOM IN-HOUSE model
Sample: ...protein monomer, 58 kDa Sulfurihydrogenibium sp. (strain … protein
Uncharacterized protein (putative MazF-like toxin) monomer, 9 kDa Sulfurihydrogenibium sp. (strain … protein
Buffer: 20 mM Tris, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Dec 14
Antiviral signalling by a cyclic nucleotide activated CRISPR protease. Nature 614(7946):168-174 (2023)
Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S, Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk JL, Svergun D, Geyer M, White MF, Hagelueken G
RgGuinier 3.4 nm
Dmax 11.7 nm
VolumePorod 93 nm3

SASDD85 – PDZK1 Domain 1-4

Uncharacterized protein C1orf159 experimental SAS data
GASBOR model
Sample: Uncharacterized protein C1orf159 monomer, 53 kDa Homo sapiens protein
Buffer: 20mM Hepes 150 NaCl 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 28
...Protein: Structure of PDZK1 in Solution. Structure 26(11):1522-1533.e5 (2018)
Hajizadeh NR, Pieprzyk J, Skopintsev P, Flayhan A, Svergun DI, Löw C
RgGuinier 3.9 nm
Dmax 15.0 nm
VolumePorod 120 nm3

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 23 nm3

SASDK85 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 7.9 nm
Dmax 27.0 nm

SASDF95 – Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex

Uncharacterized proteinDNA Duplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
DNA Duplex dimer, 20 kDa DNA
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Sep 19
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 81 nm3

SASDK95 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.330 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.0 nm
Dmax 29.0 nm

SASDKA5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.700 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 8.0 nm
Dmax 27.3 nm

SASDKB5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 1.47 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 7.9 nm
Dmax 28.0 nm

SASDKC5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.711 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 7.6 nm
Dmax 27.0 nm

SASDKD5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.373 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 7.5 nm
Dmax 26.0 nm

SASDKE5 – the Survival Motor Neuron complex (Gemin2/SMN/Gemin8/Gemin7/Gemin6) from Schizosaccharomyces pombe, 0.173 g/L

Survival motor neuron-like protein 1Uncharacterized protein C16H5.15 (Gemin8 Δ35-58)Gem-associated protein 7 homolog (Gemin7)Uncharacterized protein new12 (Gemin6)Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) experimental SAS data
Survival motor neuron-like protein 1 Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) Gem-associated protein 7 homolog (Gemin7) Uncharacterized protein new12 (Gemin6) Survival of motor neuron protein-interacting protein yip11 (Gemin2 ΔN80) Kratky plot
Sample: ...protein 1 monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein C16H5.15 (Gemin8 Δ35-58) monomer, 17 kDa Schizosaccharomyces pombe (strain … protein
...protein 7 homolog (Gemin7) monomer, 10 kDa Schizosaccharomyces pombe (strain … protein
Uncharacterized protein new12 (Gemin6) monomer, 11 kDa Schizosaccharomyces pombe (strain … protein
...protein-interacting protein yip11 (Gemin2 ΔN80) monomer, 18 kDa Schizosaccharomyces pombe (strain … protein
Buffer: 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 29
Identification and structural analysis of the Schizosaccharomyces pombe SMN complex Nucleic Acids Research (2021)
Veepaschit J, Viswanathan A, Bordonné R, Grimm C, Fischer U
RgGuinier 6.3 nm
Dmax 21.3 nm

SASDTQ5 – SemDΔAPH (apo form)

Uncharacterized protein experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 67 nm3

SASDTT5 – SemDΔAPH bound to Sorting nexin-9, N-terminus - SNX9-SH3

Uncharacterized proteinSorting nexin-9 experimental SAS data
CORAL model
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Sorting nexin-9 monomer, 19 kDa Homo sapiens protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 20
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 94 nm3

SASDTU5 – SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL)

Uncharacterized proteinActin nucleation-promoting factor WASL experimental SAS data
SemDΔAPH bound to BR-GBD (actin nucleation-promoting factor WASL) Rg histogram
Sample: Uncharacterized protein monomer, 35 kDa Chlamydia pneumoniae protein
Actin nucleation-promoting factor WASL monomer, 19 kDa Rattus norvegicus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 3 %Glycerol, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 14
The Chlamydia pneumoniae effector SemD exploits its host’s endocytic machinery by structural and functional mimicry Nature Communications 15(1) (2024)
Kocher F, Applegate V, Reiners J, Port A, Spona D, Hänsch S, Mirzaiebadizi A, Ahmadian M, Smits S, Hegemann J, Mölleken K
RgGuinier 4.2 nm
Dmax 15.9 nm
VolumePorod 102 nm3

SASDCV5 – Leishmania braziliensis p23A

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein monomer, 22 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris-HCl, 100 mM NaCl, 5 mM B-mercaptoethanol, pH: 8
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Mar 26
Identification of two p23 co-chaperone isoforms in Leishmania braziliensis exhibiting similar structures and Hsp90 interaction properties despite divergent stabilities. FEBS J 282(2):388-406 (2015)
Batista FA, Almeida GS, Seraphim TV, Silva KP, Murta SM, Barbosa LR, Borges JC
RgGuinier 3.3 nm
Dmax 13.0 nm

SASDCA6 – ...protein (DSY4693) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR2A

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein monomer, 9 kDa Desulfitobacterium hafniense protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 1.5 nm
Dmax 5.3 nm
VolumePorod 13 nm3

SASDCB6 – ...protein from Nitrosospira multiformis, Northeast Structural Genomics Consortium Target NmR72

Uncharacterized protein experimental SAS data
DAMFILT model
Sample: Uncharacterized protein tetramer, 55 kDa Nitrosospira multiformis protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.3 nm
Dmax 7.5 nm
VolumePorod 83 nm3

SASDCK6 – ...protein (DSY3957) from Desulfitobacterium hafniense, Northeast Structural Genomics Consortium Target DhR18

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein monomer, 48 kDa Desulfitobacterium hafniense protein
Buffer: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2010 Feb 12
Small angle X-ray scattering as a complementary tool for high-throughput structural studies. Biopolymers 95(8):517-30 (2011)
Grant TD, Luft JR, Wolfley JR, Tsuruta H, Martel A, Montelione GT, Snell EH
RgGuinier 2.8 nm
Dmax 9.9 nm
VolumePorod 66 nm3

SASDP27 – ...protein at 1.5 mg/ml

Uncharacterized protein, isoform A experimental SAS data
ALPHAFOLD model
Sample: Uncharacterized protein, isoform A hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
...protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 3.7 nm
Dmax 15.0 nm
VolumePorod 167 nm3

SASDP37 – ...protein at 3.0 mg/ml

Uncharacterized protein, isoform A experimental SAS data
Uncharacterized protein, isoform A Kratky plot
Sample: Uncharacterized protein, isoform A hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 8
...protein-protein interactions. Bioessays :e2200179 (2022)
Bonchuk A, Balagurov K, Georgiev P
RgGuinier 4.1 nm
Dmax 17.0 nm
VolumePorod 192 nm3

SASDQ78 – YbiB-ObgE GTPase complex

Uncharacterized protein YbiBGTPase Obg experimental SAS data
Uncharacterized protein YbiB GTPase Obg Kratky plot
Sample: Uncharacterized protein YbiB dimer, 75 kDa Escherichia coli (strain … protein
GTPase Obg dimer, 91 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2 mM DTT, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 24
YbiB: a novel interactor of the GTPase ObgE. Nucleic Acids Res (2023)
Deckers B, Vercauteren S, De Kock V, Martin C, Lazar T, Herpels P, Dewachter L, Verstraeten N, Peeters E, Ballet S, Michiels J, Galicia C, Versées W
RgGuinier 4.7 nm
Dmax 18.6 nm
VolumePorod 291 nm3

SASDU88 – ...protein MPB-MabR(Rv2242) - monomeric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: ...protein (D108A, K109A, E198A, N199A, K265A) monomer, 40 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 monomer, 43 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 2.8 nm
Dmax 8.5 nm
VolumePorod 113 nm3

SASDU98 – ...protein MPB-MabR(Rv2242) - dimeric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: ...protein (D108A, K109A, E198A, N199A, K265A) dimer, 80 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 dimer, 86 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 3.7 nm
Dmax 10.8 nm
VolumePorod 345 nm3

SASDUA8 – ...protein MPB-MabR(Rv2242) - tetrameric peak

Maltose/maltodextrin-binding periplasmic protein (D108A, K109A, E198A, N199A, K265A)Uncharacterized protein Rv2242 experimental SAS data
ALPHAFOLD model
Sample: ...protein (D108A, K109A, E198A, N199A, K265A) tetramer, 161 kDa Escherichia coli (strain … protein
Uncharacterized protein Rv2242 tetramer, 171 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, pH:
Experiment: SAXS data collected at SWING, SOLEIL on 2024 Apr 3
Domain architecture of the Mycobacterium tuberculosis MabR (Rv2242), a member of the PucR transcription factor family Heliyon :e40494 (2024)
Megalizzi V, Tanina A, Grosse C, Mirgaux M, Legrand P, Mirandela G, Wohlkönig A, Bifani P, Wintjens R
RgGuinier 5.2 nm
Dmax 15.8 nm
VolumePorod 826 nm3

SASDCT8 – Uncharacterized protein CTHT_0072540 (Core) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
DAMMIF model
Sample: ...protein CTHT_0072540 tetramer, 62 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 30
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 4.1 nm
Dmax 16.2 nm
VolumePorod 145 nm3

SASDCU8 – Uncharacterized protein CTHT_0072540 (WD40) from Chaetomium thermophilum

hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: ...protein CTHT_0072540 monomer, 35 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jul 19
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 2.3 nm
Dmax 5.8 nm
VolumePorod 68 nm3

SASDCV8 – Uncharacterized protein CTHT_0072540 (Prp19 full-length) from Chaetomium thermophilum

Full-length hypothetical protein CTHT_0072540 experimental SAS data
SASREF model
Sample: ...protein CTHT_0072540 tetramer, 207 kDa Chaetomium thermophilum protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Oct 15
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors. Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
RgGuinier 6.2 nm
Dmax 23.0 nm
VolumePorod 280 nm3

SASDRX8 – ...protein Af1318 and AfAgo-N protein (containing N-L1-L2 domains) with a 14 base pair DNA oligoduplex in low salt buffer

Piwi protein AF_1318 (Archaeoglobus fulgidus AfAgo protein)Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains)5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) experimental SAS data
CUSTOM IN-HOUSE model
Sample: ...protein AF_1318 (Archaeoglobus fulgidus AfAgo protein) monomer, 51 kDa Archaeoglobus fulgidus (strain … protein
Uncharacterized protein (AfAgo-N protein containing N-L1-L2 domains) monomer, 31 kDa Archaeoglobus fulgidus DSM … protein
5'-end phosphorylated DNA oligoduplex, 14 bp (MZ1288) monomer, 9 kDa DNA
Buffer: 20 mM TrisHCl pH 7.5, 200 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 6
...protein. Nucleic Acids Res (2024)
Manakova E, Golovinas E, Pocevičiūtė R, Sasnauskas G, Silanskas A, Rutkauskas D, Jankunec M, Zagorskaitė E, Jurgelaitis E, Grybauskas A, Venclovas Č, Zaremba M
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 145 nm3

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: ...protein ParE 16-mer, 188 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) 16-mer, 135 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 9
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.8 nm
Dmax 16.2 nm
VolumePorod 312 nm3

SASDP49 – ...protein at 1.5 mg/ml

Uncharacterized protein, isoform A experimental SAS data
ALPHAFOLD model
Sample: Uncharacterized protein, isoform A hexamer, 92 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, pH 7.4, 200 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 20
...protein domains use previously unknown interface to form hexamers. Elife 13 (2024)
Bonchuk AN, Balagurov KI, Baradaran R, Boyko KM, Sluchanko NN, Khrustaleva AM, Burtseva AD, Arkova OV, Khalisova KK, Popov VO, Naschberger A, Georgiev PG
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 166 nm3

SASDAB9 – EcPaaA2-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: ...protein ParE octamer, 102 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) octamer, 68 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 3.3 nm
Dmax 15.3 nm
VolumePorod 166 nm3

SASDC84 – parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct

Plasmid stabilization protein ParEUncharacterized protein experimental SAS data
parDE-like toxin-antitoxin module, EcPaaA2_13-63-HisEcParE2 construct Rg histogram
Sample: ...protein ParE monomer, 13 kDa Escherichia coli protein
Uncharacterized protein monomer, 6 kDa Escherichia coli O157:H7 protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 Feb 5
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
Sterckx YG, Jové T, Shkumatov AV, Garcia-Pino A, Geerts L, De Kerpel M, Lah J, De Greve H, Van Melderen L, Loris R
RgGuinier 2.2 nm
Dmax 9.3 nm
VolumePorod 36 nm3