Small Angle Scattering Biological Data Bank SASBDB


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27 hits found for Lisboa

SASDTG2 – LCP domain of LytR from Streptococcus dysgalactiae in the presence of geranylgeranyl diphosphate (150 mM NaCl)

Biofilm regulatory protein experimental SAS data

Sample:	Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 1

Structural insights of an LCP protein-LytR-from Streptococcus dysgalactiae subs. dysgalactiae through biophysical and in silico methods. Front Chem 12:1379914 (2024)
Paquete-Ferreira J, Freire F, Fernandes HS, Muthukumaran J, Ramos J, Bryton J, Panjkovich A, Svergun D, Santos MFA, Correia MAS, Fernandes AR, Romão MJ, Sousa SF, Santos-Silva T

R_g^Guinier	2.5	nm
D_max	8.0	nm
Volume^Porod	83	nm³

SASDTH2 – LCP domain of LytR from Streptococcus dysgalactiae in the absence of ligand (150 mM NaCl)

Sample:	Biofilm regulatory protein monomer, 34 kDa Streptococcus dysgalactiae subsp. … protein
Buffer:	50 mM HEPES, 150 mM NaCl, 5 mM MgCl2, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2022 Jun 1

R_g^Guinier	2.4	nm
D_max	9.0	nm
Volume^Porod	76	nm³

SASDF34 – Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) experimental SAS data

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Jun 20

Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Structure (2019)
Cordeiro TN, Sibille N, Germain P, Barthe P, Boulahtouf A, Allemand F, Bailly R, Vivat V, Ebel C, Barducci A, Bourguet W, le Maire A, Bernadó P

R_g^Guinier	4.7	nm
D_max	17.7	nm
Volume^Porod	102	nm³

SASDF44 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1)

Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD)Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) experimental SAS data

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Jul 23

R_g^Guinier	4.8	nm
D_max	19.4	nm
Volume^Porod	167	nm³

SASDF54 – RXRΔH12/RAR Heterodimer : N-CoRNID Complex (1:1)

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Δ helix12 monomer, 24 kDa Mus musculus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Jul 23

R_g^Guinier	4.2	nm
D_max	15.7	nm
Volume^Porod	183	nm³

SASDF64 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1) with RAR inverse agonist (BMS493)

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Jul 23

R_g^Guinier	4.8	nm
D_max	19.5	nm
Volume^Porod	178	nm³

SASDF74 – RXR/RARI396E Heterodimer : N-CoRNID Complex (1:1)

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) mutant I396E monomer, 28 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2015 Mar 9

R_g^Guinier	5.3	nm
D_max	22.4	nm
Volume^Porod	171	nm³

SASDF84 – RXR/RAR Heterodimer : N-CoRNID Complex (1:1) with RAR agonist (Am580)

Sample:	Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) monomer, 29 kDa Mus musculus protein Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) monomer, 26 kDa Mus musculus protein Retinoic acid receptor alpha (RAR-alpha) Ligand binding domain (LDB) monomer, 28 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2014 Jul 23

R_g^Guinier	4.2	nm
D_max	17.2	nm
Volume^Porod	131	nm³

SASDNU5 – Lysin from Streptococcus phage P7951 ∆1-208

lysin [Streptococcus phage P7951] experimental SAS data

Sample:	lysin [Streptococcus phage P7951] hexamer, 65 kDa Streptococcus phage P7951 protein
Buffer:	50 mM HEPES, 500 mM NaCl, and 1% glycerol, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2021 Nov 21

On the Occurrence and Multimerization of Two-Polypeptide Phage Endolysins Encoded in Single Genes. Microbiol Spectr :e0103722 (2022)
Pinto D, Gonçalo R, Louro M, Silva MS, Hernandez G, Cordeiro TN, Cordeiro C, São-José C

R_g^Guinier	3.1	nm
D_max	10.1	nm
Volume^Porod	111	nm³

SASDNW6 – AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida

Apoptosis inducing protein experimental SAS data

Sample:	Apoptosis inducing protein monomer, 57 kDa Photobacterium damselae subsp. … protein
Buffer:	50 mM Hepes, 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Apr 13

Unconventional structure and mechanisms for membrane interaction and translocation of the NF-κB-targeting toxin AIP56. Nat Commun 14(1):7431 (2023)
Lisboa J, Pereira C, Pinto RD, Rodrigues IS, Pereira LMG, Pinheiro B, Oliveira P, Pereira PJB, Azevedo JE, Durand D, Benz R, do Vale A, Dos Santos NMS

R_g^Guinier	2.8	nm
D_max	9.5	nm
Volume^Porod	76	nm³

SASDFB7 – Human phenylalanine hydroxylase (hPAH)

Phenylalanine-4-hydroxylase experimental SAS data

Sample:	Phenylalanine-4-hydroxylase tetramer, 223 kDa Homo sapiens protein
Buffer:	0.02 mM Hepes, 0.2 M NaCl,, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Feb 25

Modulation of Human Phenylalanine Hydroxylase by 3-Hydroxyquinolin-2(1H)-One Derivatives Biomolecules 11(3):462 (2021)
Lopes R, Tomé C, Russo R, Paterna R, Leandro J, Candeias N, Gonçalves L, Teixeira M, Sousa P, Guedes R, Vicente J, Gois P, Leandro P

R_g^Guinier	4.3	nm
D_max	14.8	nm
Volume^Porod	336	nm³

SASDFC7 – Human phenylalanine hydroxylase (hPAH) + 1 mM L-Phe

Sample:	Phenylalanine-4-hydroxylase tetramer, 223 kDa Homo sapiens protein
Buffer:	0.02 mM Hepes, 0.2 M NaCl,, pH: 7
Experiment:	SAXS data collected at B21, Diamond Light Source on 2016 Feb 25

R_g^Guinier	4.0	nm
D_max	12.3	nm
Volume^Porod	367	nm³

SASDLC7 – Polyribonucleotide nucleotidyltransferase (PNPase): apo form

Polyribonucleotide nucleotidyltransferase experimental SAS data

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 246 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris-HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity. Biochimie (2023)
Bárria C, Athayde D, Hernandez G, Fonseca L, Casinhas J, Cordeiro TN, Archer M, Arraiano CM, Brito JA, Matos RG

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	310	nm³

SASDLD7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-AMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1 mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	315	nm³

SASDLE7 – Polyribonucleotide nucleotidyltransferase (PNPase): 2-GMP complex

Sample:	Polyribonucleotide nucleotidyltransferase trimer, 237 kDa Campylobacter jejuni subsp. … protein
Buffer:	20 mM Tris.HCl, 10 mM NAH2PO4, 60 mM KCl, 1mM MgCl2, 2 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2020 Mar 17

R_g^Guinier	3.9	nm
D_max	11.0	nm
Volume^Porod	312	nm³

SASDJ28 – Ferric Iron Reductase - FhuF∆1-17

Ferric iron reductase protein FhuF (∆1-17) experimental SAS data

Sample:	Ferric iron reductase protein FhuF (∆1-17) monomer, 28 kDa Escherichia coli (strain … protein
Buffer:	20 mM Phosphate, 200 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Apr 16

Conjuring up a ghost: structural and functional characterization of FhuF, a ferric siderophore reductase from E. coli JBIC Journal of Biological Inorganic Chemistry (2021)
Trindade I, Hernandez G, Lebègue E, Barrière F, Cordeiro T, Piccioli M, Louro R

R_g^Guinier	2.1	nm
D_max	8.8	nm
Volume^Porod	60	nm³

SASDHL8 – Aquifex aeolicus McoA metaloxidase evolved variant (2F4)

McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) experimental SAS data

Sample:	McoA evolved variant 2F4 (Periplasmic cell division protein (SufI)) monomer, 55 kDa Aquifex aeolicus VF5 protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Sep 25

Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase ACS Catalysis :5022-5035 (2022)
Brissos V, Borges P, Núñez-Franco R, Lucas M, Frazão C, Monza E, Masgrau L, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	6.8	nm
Volume^Porod	78	nm³

SASDHM8 – Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352) experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆328-352 evolved variant (2F4∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Jul 18

R_g^Guinier	2.2	nm
D_max	6.6	nm
Volume^Porod	74	nm³

SASDBG7 – Dps1, DNA binding protein under starvation conditions (SEC-SAXS)

DNA protection during starvation protein 1 experimental SAS data

Sample:	DNA protection during starvation protein 1 dodecamer, 276 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions. J Mol Biol 429(5):667-687 (2017)
Santos SP, Cuypers MG, Round A, Finet S, Narayanan T, Mitchell EP, Romão CV

R_g^Guinier	4.2	nm
D_max	12.8	nm
Volume^Porod	437	nm³

SASDFY7 – Aquifex aeolicus McoA metaloxidase

Aquifex aeolicus McoA metaloxidase experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Apr 15

The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis ACS Catalysis (2020)
Borges P, Brissos V, Hernandez G, Masgrau L, Lucas M, Monza E, Frazão C, Cordeiro T, Martins L

R_g^Guinier	2.3	nm
D_max	7.5	nm
Volume^Porod	79	nm³

SASDKF8 – N-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

Translocated intimin receptor Tir experimental SAS data

Sample:	Translocated intimin receptor Tir dimer, 50 kDa Escherichia coli O127:H6 … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Feb 18

The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection. Commun Biol 7(1):179 (2024)
Vieira MFM, Hernandez G, Zhong Q, Arbesú M, Veloso T, Gomes T, Martins ML, Monteiro H, Frazão C, Frankel G, Zanzoni A, Cordeiro TN

R_g^Guinier	3.5	nm
D_max	14.0	nm
Volume^Porod	46	nm³

SASDBH7 – Dps1 truncated, DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 1 experimental SAS data

Sample:	N-terminal truncated DNA protection during starvation protein 1 dodecamer, 216 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	3.9	nm
D_max	10.0	nm
Volume^Porod	291	nm³

SASDFX7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆337-346 (MCoA∆337-346)

Aquifex aeolicus McoA metaloxidase ∆337-346 experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆337-346 monomer, 54 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2017 Dec 4

R_g^Guinier	2.3	nm
D_max	7.0	nm
Volume^Porod	78	nm³

SASDRQ5 – Small beta-barrel domain in the N-terminal region of the Translocated Intimin Receptor (Tir76-180)

Translocated intimin receptor Tir Kratky plot

Sample:	Translocated intimin receptor Tir monomer, 12 kDa Escherichia coli O127:H6 … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2021 Nov 21

R_g^Guinier	1.7	nm
D_max	7.0	nm
Volume^Porod	21	nm³

SASDBF7 – Dps2, DNA binding protein under starvation conditions (SEC-SAXS)

N-terminal truncated DNA protection during starvation protein 2 experimental SAS data

Sample:	N-terminal truncated DNA protection during starvation protein 2 dodecamer, 279 kDa Deinococcus radiodurans R1 protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Nov 22

R_g^Guinier	4.2	nm
D_max	12.7	nm
Volume^Porod	445	nm³

SASDFW7 – Aquifex aeolicus McoA metaloxidase deletion mutant ∆328-352 (MCoA∆328-352)

Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) experimental SAS data

Sample:	Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) monomer, 53 kDa Aquifex aeolicus protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2017 Jul 13

R_g^Guinier	2.3	nm
D_max	6.9	nm
Volume^Porod	77	nm³

SASDKH8 – C-Terminal Cytoplasmatic Region of the Translocated Intimin Receptor

Sample:	Translocated intimin receptor Tir monomer, 18 kDa Escherichia coli O127:H6 … protein
Buffer:	20 mM Sodium Phosphate, 150 mM NaCl, 1 mM EDTA, pH: 6.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Sep 15

R_g^Guinier	3.8	nm
D_max	12.8	nm
Volume^Porod	46	nm³

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