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14 hits found for Spiteri

SASDU52 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, apo form

Cereblon-midi experimental SAS data
SWISSMODEL model
Sample: Cereblon-midi monomer, 37 kDa protein
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 61 nm3

SASDU62 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Mezigdomide

Cereblon-midimezigdomide experimental SAS data
PYMOL model
Sample: Cereblon-midi monomer, 37 kDa protein
mezigdomide monomer, 1 kDa synthetic construct
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 60 nm3

SASDU72 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Pomalidomide

Cereblon-midipomalidomide experimental SAS data
Cereblon-midi pomalidomide Kratky plot
Sample: Cereblon-midi monomer, 37 kDa protein
pomalidomide monomer, 0 kDa synthetic construct
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.3 nm
Dmax 6.9 nm
VolumePorod 63 nm3

SASDU82 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Iberdomide

Cereblon-midiIberdomide experimental SAS data
Cereblon-midi Iberdomide Kratky plot
Sample: Cereblon-midi monomer, 37 kDa protein
Iberdomide monomer, 0 kDa synthetic construct
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.4 nm
Dmax 7.7 nm
VolumePorod 68 nm3

SASDU92 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Lenalidomide

Cereblon-midiLenalidomide experimental SAS data
Cereblon-midi Lenalidomide Kratky plot
Sample: Cereblon-midi monomer, 37 kDa protein
Lenalidomide monomer, 0 kDa synthetic construct
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.2 nm
Dmax 6.2 nm
VolumePorod 59 nm3

SASDLX2 – Immunoglobulin G4 (IgG4) Deglycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4. PLoS One 19(4):e0300964 (2024)
Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ
RgGuinier 4.9 nm
Dmax 16.9 nm
VolumePorod 258 nm3

SASDLY2 – Immunoglobulin G4 (IgG4) Glycosylated

Immunoglobulin G subclass 4 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 4 monomer, 145 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Using atomistic solution scattering modelling to elucidate the role of the Fc glycans in human IgG4. PLoS One 19(4):e0300964 (2024)
Spiteri VA, Doutch J, Rambo RP, Bhatt JS, Gor J, Dalby PA, Perkins SJ
RgGuinier 4.9 nm
Dmax 16.3 nm
VolumePorod 256 nm3

SASDLZ2 – Immunoglobulin G3 (IgG3) Glycosylated

Immunoglobulin G subclass 3 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 3 monomer, 155 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
Solution structures of human myeloma IgG3 antibody reveal extended Fab and Fc regions relative to the other IgG subclasses. J Biol Chem :100995 (2021)
Spiteri VA, Goodall M, Doutch J, Rambo RP, Gor J, Perkins SJ
RgGuinier 7.0 nm
Dmax 22.9 nm
VolumePorod 267 nm3

SASDL23 – Immunoglobulin G3 (IgG3) Deglycosylated

Immunoglobulin G subclass 3 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 3 monomer, 155 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Jul 29
Solution structures of human myeloma IgG3 antibody reveal extended Fab and Fc regions relative to the other IgG subclasses. J Biol Chem :100995 (2021)
Spiteri VA, Goodall M, Doutch J, Rambo RP, Gor J, Perkins SJ
RgGuinier 7.1 nm
Dmax 27.7 nm
VolumePorod 294 nm3

SASDNV5 – Botulinum Toxin E at acidic pH

Botulinum Toxin Serotype E, endonegative experimental SAS data
GROMACS model
Sample: Botulinum Toxin Serotype E, endonegative monomer, 144 kDa Clostridium botulinum protein
Buffer: 10 mM sodium acetate (20 mM ionic strength), pH: 4
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 6
Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E. J Struct Biol :107876 (2022)
...Spiteri VA, Perkins SJ, Dalby PA
RgGuinier 4.3 nm
Dmax 15.2 nm
VolumePorod 240 nm3

SASDVN6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-VcN

Cereblon-midi experimental SAS data
Cereblon-midi Kratky plot
Sample: Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer: 20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 29
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.3 nm
Dmax 9.1 nm
VolumePorod 63 nm3

SASDVP6 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Boc-AcQ

Cereblon-midi experimental SAS data
Cereblon-midi Kratky plot
Sample: Cereblon-midi monomer, 37 kDa synthetic construct protein
Buffer: 20 mM HEPES, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Jun 29
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
...Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 62 nm3

SASDKB8 – Immunoglobulin G1 (IgG1) Glycosylated

Immunoglobulin G subclass 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 1 , 148 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation Biophysical Journal (2021)
Spiteri V, Doutch J, Rambo R, Gor J, Dalby P, Perkins S
RgGuinier 5.1 nm
Dmax 17.5 nm
VolumePorod 269 nm3

SASDKC8 – Immunoglobulin G1 (IgG1) Deglycosylated

Immunoglobulin G subclass 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Immunoglobulin G subclass 1 , 148 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, and 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 20
Solution structure of deglycosylated human IgG1 shows the role of CH2 glycans in its conformation Biophysical Journal (2021)
Spiteri V, Doutch J, Rambo R, Gor J, Dalby P, Perkins S
RgGuinier 5.2 nm
Dmax 18.4 nm
VolumePorod 271 nm3