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49 hits found for Jensen

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDF42 – Human serum albumin in complex with somapacitan

Human serum albuminSomapacitan experimental SAS data
SASREF model
Sample: Human serum albumin monomer, 66 kDa Homo sapiens protein
Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer: 100 mM MES, 140 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Jensen S, Petersen J, Thygesen P
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 202 nm3

SASDD62 – Microtubule associated protein MAP2c (isoform 3); 12 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 8.3 nm

SASDD72 – Microtubule associated protein MAP2c (isoform 3); 6 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 7.9 nm

SASDD82 – Microtubule associated protein MAP2c (isoform 3); 3 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 6.9 nm

SASDD92 – Microtubule associated protein MAP2c (isoform 3); 1.5 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 6.7 nm

SASDDA2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 13.6 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 7.9 nm

SASDDB2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 6.8 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: 50 mM MOPS, 150 mM NaCl, 0.03% NaN3, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 7.3 nm

SASDDC2 – Phosphorylated Microtubule associated protein MAP2c (isoform 3); 1.7 mg/ml

Microtubule-associated protein 2, isoform 3 experimental SAS data
Microtubule-associated protein 2, isoform 3 Kratky plot
Sample: Microtubule-associated protein 2, isoform 3 monomer, 49 kDa Rattus norvegicus protein
Buffer: MOPS map2c buffer, pH: 6.9
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 21
Functionally specific binding regions of microtubule-associated protein 2c exhibit distinct conformations and dynamics. J Biol Chem 293(34):13297-13309 (2018)
...Jensen MR, Blackledge M, Žídek L
RgGuinier 6.7 nm

SASDMM2 – Thalassiosira pseudonana iota Carbonic anhydrase (ι-CA)

iota carbonic anhydrase experimental SAS data
DAMMIF model
Sample: iota carbonic anhydrase tetramer, 260 kDa Thalassiosira pseudonana protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 28
Structural Contour Map of the Iota Carbonic Anhydrase from the Diatom Thalassiosira pseudonana Using a Multiprong Approach. Int J Mol Sci 22(16) (2021)
Jensen EL, Receveur-Brechot V, Hachemane M, Wils L, Barbier P, Parsiegla G, Gontero B, Launay H
RgGuinier 6.7 nm
Dmax 25.0 nm
VolumePorod 500 nm3

SASDTU2 – NAD-dependent protein deacetylase sirtuin-7 at pH 8.0

Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 experimental SAS data
CORAL model
Sample: Isoform 1 of NAD-dependent protein deacetylase sirtuin-7 monomer, 45 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 16
Substrates and Cyclic Peptide Inhibitors of the Oligonucleotide-Activated Sirtuin 7. Angew Chem Int Ed Engl :e202314597 (2023)
...Jensen I, Hansen TN, Ryberg LA, Jameson ST, Harris P, Peters GHJ, Denu JM, Rogers JM, Olsen CA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 89 nm3

SASDEF3 – Paenibacillus xanthan lyase (PXL) at 4 °C

Paenibacillus xanthan lyase experimental SAS data
Paenibacillus xanthan lyase Kratky plot
Sample: Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein
Buffer: 20 mM Tris,, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 15
Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity. Cell Chem Biol 26(2):191-202.e6 (2019)
Jensen PF, Kadziola A, Comamala G, Segura DR, Anderson L, Poulsen JN, Rasmussen KK, Agarwal S, Sainathan RK, Monrad RN, Svendsen A, Nielsen JE, Lo Leggio L, Rand KD
RgGuinier 3.7 nm
Dmax 13.1 nm
VolumePorod 137 nm3

SASDEG3 – Paenibacillus xanthan lyase (PXL) at 20 °C

Paenibacillus xanthan lyase experimental SAS data
Paenibacillus xanthan lyase Kratky plot
Sample: Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein
Buffer: 20 mM Tris,, pH: 8.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 15
Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity. Cell Chem Biol 26(2):191-202.e6 (2019)
Jensen PF, Kadziola A, Comamala G, Segura DR, Anderson L, Poulsen JN, Rasmussen KK, Agarwal S, Sainathan RK, Monrad RN, Svendsen A, Nielsen JE, Lo Leggio L, Rand KD
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3

SASDUQ4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 15.2 nm
VolumePorod 367 nm3

SASDUR4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 14.9 nm
VolumePorod 351 nm3

SASDUS4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (i)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDHT4 – Phosphoprotein from Nipah virus

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein tetramer, 317 kDa Nipah henipavirus protein
Buffer: 20 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at ID14-3, ESRF on 2011 Nov 3
Structural Description of the Nipah Virus Phosphoprotein and Its Interaction with STAT1. Biophys J (2020)
Jensen MR, Yabukarski F, Communie G, Condamine E, Mas C, Volchkova V, Tarbouriech N, Bourhis JM, Volchkov V, Blackledge M, Jamin M
RgGuinier 10.9 nm
Dmax 39.3 nm

SASDUT4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 5.5 nm
Dmax 27.0 nm

SASDUU4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 31
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 8.8 nm
Dmax 37.0 nm

SASDUV4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iii)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 556 nm3

SASDUW4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iv)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 15.6 nm
Dmax 90.0 nm

SASDUX4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 17.5 nm
VolumePorod 372 nm3

SASDUY4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with HOCPCA

Calcium/calmodulin-dependent protein kinase type II subunit alpha3-hydroxycyclopent-1-enecarboxylic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha 3-hydroxycyclopent-1-enecarboxylic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
3-hydroxycyclopent-1-enecarboxylic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 1 mM HOCPCA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.1 nm
Dmax 12.7 nm
VolumePorod 310 nm3

SASDUZ4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 16.5 nm
VolumePorod 358 nm3

SASDU25 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 15.0 nm
VolumePorod 334 nm3

SASDU35 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L, Département de Biochimie, Université de Montréal on 2022 Dec 15
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 15.0 nm
VolumePorod 335 nm3

SASDU45 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 329 nm3

SASDU55 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 14.5 nm
VolumePorod 313 nm3

SASDU65 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 20.0 nm
VolumePorod 375 nm3

SASDU75 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)5-hydroxydiclofenac experimental SAS data
Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 5-hydroxydiclofenac Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
5-hydroxydiclofenac monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
...Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.7 nm
Dmax 18.5 nm
VolumePorod 366 nm3

SASDGA5 – The C-terminal cell-surface signaling domain of the Pseudomonas capeferrum anti-sigma regulator PupR

PupR protein experimental SAS data
MULTIFOXS model
Sample: PupR protein monomer, 24 kDa Pseudomonas putida protein
Buffer: 25 mM HEPES 400 mM LiCl 10% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Mar 16
Structural basis of cell surface signaling by a conserved sigma regulator in Gram-negative bacteria. J Biol Chem (2020)
Jensen JL, Jernberg BD, Sinha S, Colbert CL
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 49 nm3

SASDSG5 – Human MUC2 mucin CysD2 domain

Mucin-2 (CysD2 domain) experimental SAS data
Mucin-2 (CysD2 domain) Kratky plot
Sample: Mucin-2 (CysD2 domain) monomer, 16 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 70 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Mar 8
The structure of the second CysD domain of MUC2 and role in mucin organization by transglutaminase-based cross-linking. Cell Rep 43(5):114207 (2024)
...Jensen M, Lymer R, Bäckström M, Johansson MEV, Hansson GC, Trillo-Muyo S
RgGuinier 2.5 nm
Dmax 9.3 nm
VolumePorod 31 nm3

SASDTP5 – Invariant surface glycoprotein 75 (N134A) amino acids 29-468

Invariant surface glycoprotein (N134A) experimental SAS data
CORAL model
Sample: Invariant surface glycoprotein (N134A) monomer, 49 kDa Trypanosoma brucei protein
Buffer: 20 mM Tris-HCl, 75 mM KCl, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 18
Trypanosoma brucei Invariant Surface Glycoprotein 75 Is an Immunoglobulin Fc Receptor Inhibiting Complement Activation and Antibody-Mediated Cellular Phagocytosis. J Immunol (2024)
...Jensen MP, Hansen AG, Wu Q, Lorentzen J, Graversen JH, Andersen GR, Fenton RA, Etzerodt A, Thiel S, Andersen CBF
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 127 nm3

SASDGU5 – The C-terminal cell-surface signaling domain of the Pseudomonas capeferrum anti-sigma regulator PupR in complex with the outer membrane transporter PupB N-terminal signaling domain

PupR proteinFerric-pseudobactin BN7/BN8 receptor experimental SAS data
DAMFILT model
Sample: PupR protein monomer, 24 kDa Pseudomonas putida protein
Ferric-pseudobactin BN7/BN8 receptor monomer, 8 kDa Pseudomonas putida protein
Buffer: 25 mM HEPES 400 mM LiCl 10% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Mar 16
Structural basis of cell surface signaling by a conserved sigma regulator in Gram-negative bacteria. J Biol Chem (2020)
Jensen JL, Jernberg BD, Sinha S, Colbert CL
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 56 nm3

SASDR86 – Human Survivin (BIRC5)

Baculoviral IAP repeat-containing protein 5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Baculoviral IAP repeat-containing protein 5 dimer, 34 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Jan 10
Survivin prevents the Polycomb Repressor Complex 2 from methylating Histone 3 lysine 27 iScience :106976 (2023)
Jensen M, Chandrasekaran V, García-Bonete M, Li S, Anindya A, Andersson K, Erlandsson M, Oparina N, Burmann B, Brath U, Panchenko A, Maria Bokarewa I, Katona G
RgGuinier 2.9 nm
Dmax 10.4 nm
VolumePorod 53 nm3

SASDBZ7 – Complement factor 1s in complex with Complement factor 1r

Complement C1r subcomponentComplement C1s subcomponent experimental SAS data
CORAL model
Sample: Complement C1r subcomponent dimer, 156 kDa Homo sapiens protein
Complement C1s subcomponent dimer, 150 kDa Homo sapiens protein
Buffer: 50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 8
Structure and activation of C1, the complex initiating the classical pathway of the complement cascade. Proc Natl Acad Sci U S A 114(5):986-991 (2017)
...Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR
RgGuinier 11.6 nm

SASDB28 – Complement factor 1q (C1q)

Complement C1q subcomponent subunit CComplement C1q subcomponent subunit BComplement C1q subcomponent subunit A experimental SAS data
Complement C1q subcomponent subunit C Complement C1q subcomponent subunit B Complement C1q subcomponent subunit A Kratky plot
Sample: Complement C1q subcomponent subunit C hexamer, 142 kDa Homo sapiens protein
Complement C1q subcomponent subunit B hexamer, 142 kDa Homo sapiens protein
Complement C1q subcomponent subunit A hexamer, 142 kDa Homo sapiens protein
Buffer: 50 mM TrisHCl, 145 mM NaCl, 3 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 8
Structure and activation of C1, the complex initiating the classical pathway of the complement cascade. Proc Natl Acad Sci U S A 114(5):986-991 (2017)
...Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR
RgGuinier 12.6 nm

SASDB38 – Inactivated complement factor 1 (C1)

Complement C1q subcomponent subunit CComplement C1q subcomponent subunit BComplement C1q subcomponent subunit AComplement C1r subcomponentComplement C1s subcomponent experimental SAS data
CORAL model
Sample: Complement C1q subcomponent subunit C hexamer, 142 kDa Homo sapiens protein
Complement C1q subcomponent subunit B hexamer, 142 kDa Homo sapiens protein
Complement C1q subcomponent subunit A hexamer, 142 kDa Homo sapiens protein
Complement C1r subcomponent dimer, 156 kDa Homo sapiens protein
Complement C1s subcomponent dimer, 150 kDa Homo sapiens protein
Buffer: 50 mM EPPS, 145 mM NaCl, 3 mM CaCl2, pH: 8.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16
Structure and activation of C1, the complex initiating the classical pathway of the complement cascade. Proc Natl Acad Sci U S A 114(5):986-991 (2017)
...Jensen RK, Pedersen JS, Golas MM, Jensenius JC, Hansen AG, Thiel S, Andersen GR
RgGuinier 11.5 nm
Dmax 36.6 nm

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 3.5 nm
Dmax 14.0 nm
VolumePorod 38 nm3

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 3.9 nm
Dmax 20.0 nm
VolumePorod 56 nm3

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.2 nm
Dmax 20.0 nm
VolumePorod 63 nm3

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl

DNA-directed RNA polymerase subunit delta experimental SAS data
DNA-directed RNA polymerase subunit delta Kratky plot
Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.5 nm
Dmax 21.0 nm
VolumePorod 77 nm3

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.3 nm
Dmax 19.5 nm
VolumePorod 58 nm3

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.6 nm
Dmax 22.0 nm
VolumePorod 76 nm3

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.5 nm
Dmax 24.0 nm
VolumePorod 78 nm3

SASDFC8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl

DNA-directed RNA polymerase subunit delta - mutant experimental SAS data
DNA-directed RNA polymerase subunit delta - mutant Kratky plot
Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein
Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3
Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase. J Am Chem Soc (2019)
...Jensen MR, Blackledge M, Zidek L
RgGuinier 4.5 nm
Dmax 21.0 nm
VolumePorod 74 nm3

SASDB59 – Recombinant monomeric human Properdin

Human recombinant Properdin TSR 0-3Human recombinant Properdin TSR 4-6 experimental SAS data
CORAL model
Sample: Human recombinant Properdin TSR 0-3 monomer, 25 kDa Homo sapiens protein
Human recombinant Properdin TSR 4-6 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 16
Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
...Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR
RgGuinier 5.1 nm
Dmax 18.0 nm

SASDB69 – E244K monomeric human Properdin

E244K Human Properdin experimental SAS data
CORAL model
Sample: E244K Human Properdin monomer, 49 kDa Homo sapiens protein
Buffer: 10 mM HEPES 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jul 27
Functional and structural insight into properdin control of complement alternative pathway amplification. EMBO J 36(8):1084-1099 (2017)
...Jensen RK, Gadeberg TA, Marinozzi C, Picard C, Rybkine T, Thiel S, Sørensen UB, Stover C, Fremeaux-Bacchi V, Andersen GR
RgGuinier 4.1 nm
Dmax 15.0 nm