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40 hits found for Gao

SASDT92 – Prion-like domain of 29 kDa chloroplastic ribonucleoprotein measured at at 298 K from Arabidopsis thaliana (Mouse-ear cress)

29 kDa ribonucleoprotein, chloroplastic experimental SAS data
29 kDa ribonucleoprotein, chloroplastic Kratky plot
Sample: 29 kDa ribonucleoprotein, chloroplastic monomer, 8 kDa Arabidopsis thaliana protein
Buffer: 20 mM sodium phosphate, 30 mM NaCl, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Oct 19
A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis. Plant Cell (2024)
...Gao Y, Mergenthal S, Weber W, Klotzsch E, Zoschke R, Sattler M, Schmitz-Linneweber C
RgGuinier 2.1 nm
Dmax 9.2 nm
VolumePorod 19 nm3

SASDTA2 – Prion-like domain of 29 kDa chloroplastic ribonucleoprotein measured at at 277 K from Arabidopsis thaliana (Mouse-ear cress)

29 kDa ribonucleoprotein, chloroplastic experimental SAS data
29 kDa ribonucleoprotein, chloroplastic Kratky plot
Sample: 29 kDa ribonucleoprotein, chloroplastic monomer, 8 kDa Arabidopsis thaliana protein
Buffer: 20 mM sodium phosphate, 30 mM NaCl, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Oct 19
A prion-like domain is required for phase separation and chloroplast RNA processing during cold acclimation in Arabidopsis. Plant Cell (2024)
...Gao Y, Mergenthal S, Weber W, Klotzsch E, Zoschke R, Sattler M, Schmitz-Linneweber C
RgGuinier 2.4 nm
Dmax 11.6 nm
VolumePorod 25 nm3

SASDSD2 – Recombinant Heavy-chain-only nanobody C5-Fc (glycosylated)

Immunoglobulin heavy constant gamma 1 experimental SAS data
PYMOL model
Sample: Immunoglobulin heavy constant gamma 1 monomer, 78 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Sep 28
The solution structure of the heavy chain-only C5-Fc nanobody reveals exposed variable regions that are optimal for COVID-19 antigen interactions. J Biol Chem :105337 (2023)
Gao X, Thrush JW, Gor J, Naismith JH, Owens RJ, Perkins SJ
RgGuinier 3.9 nm
Dmax 13.2 nm
VolumePorod 157 nm3

SASDSE2 – Recombinant Heavy-chain-only nanobody C5-Fc (Deglycosylated)

Immunoglobulin heavy constant gamma 1 experimental SAS data
PYMOL model
Sample: Immunoglobulin heavy constant gamma 1 monomer, 78 kDa Homo sapiens protein
Buffer: 20 mM L-histidine, 138 mM NaCl, 2.6 mM KCl buffer, pH: 6
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 Sep 28
The solution structure of the heavy chain-only C5-Fc nanobody reveals exposed variable regions that are optimal for COVID-19 antigen interactions. J Biol Chem :105337 (2023)
Gao X, Thrush JW, Gor J, Naismith JH, Owens RJ, Perkins SJ
RgGuinier 4.0 nm
Dmax 13.1 nm
VolumePorod 175 nm3

SASDUT2 – Recombinant short complement regulator SCR-17/18H (produced in Pichia pastoris with PNGase F treatment)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 17 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
The SCR-17 and SCR-18 glycans in human complement Factor H enhance its regulatory function. J Biol Chem :107624 (2024)
Gao X, Iqbal H, Yu DQ, Gor J, Coker AR, Perkins SJ
RgGuinier 3.1 nm
Dmax 10.5 nm
VolumePorod 46 nm3

SASDUU2 – Recombinant short complement regulator SCR-17/18, without glycan (produced in Escherichia coli)

Complement factor H experimental SAS data
PYMOL model
Sample: Complement factor H monomer, 14 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 137 mM NaCl, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 20
The SCR-17 and SCR-18 glycans in human complement Factor H enhance its regulatory function. J Biol Chem :107624 (2024)
Gao X, Iqbal H, Yu DQ, Gor J, Coker AR, Perkins SJ
RgGuinier 2.3 nm
Dmax 7.0 nm
VolumePorod 19 nm3

SASDF94 – Insulin glulisine (Apidra), oligomeric composition

Insulin glulisine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Insulin glulisine hexamer, 35 kDa protein
Buffer: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H
RgGuinier 2.3 nm
Dmax 7.6 nm

SASDFA4 – Insulin glargine (Toujeo®), oligomeric composition

Insulin glargine (Toujeo®) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Insulin glargine (Toujeo®) hexamer, 36 kDa protein
Buffer: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 6.2 nm

SASDFB4 – Insulin glargine (Lantus ®), oligomeric mixture

Insulin glargine (Lantus ®) experimental SAS data
Insulin glargine (Lantus ®) Kratky plot
Sample: Insulin glargine (Lantus ®) hexamer, 36 kDa protein
Buffer: Lantus Formulation (30 µg Zinc cloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 23
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
...Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H
RgGuinier 1.8 nm
Dmax 5.3 nm

SASDLF4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C224S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 3.9 nm
Dmax 14.2 nm
VolumePorod 135 nm3

SASDLG4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S+C225S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C224S/C225S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 4.2 nm
Dmax 15.1 nm
VolumePorod 135 nm3

SASDLH4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C225S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 4.0 nm
Dmax 14.5 nm
VolumePorod 131 nm3

SASDLJ4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant

ChiLob 7/4 IgG2 F(ab)2 C228S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C228S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C228S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 4.1 nm
Dmax 13.5 nm
VolumePorod 135 nm3

SASDLK4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C224S, kappa chain C214S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C224S/kC214S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 133 nm3

SASDLL4 – Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant

ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant experimental SAS data
Human immunoglobulin gamma 2 (IgG2) ChiLob 7/4 F(ab)2 - Heavy chain C225S, kappa chain C214S mutant Rg histogram
Sample: ChiLob 7/4 IgG2 F(ab)2 C225S/kC214S mutant monomer, 99 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Feb 9
Hinge disulfides in human IgG2 CD40 antibodies modulate receptor signaling by regulation of conformation and flexibility Science Immunology 7(73) (2022)
...Gao Y, Duriez P, Booth S, Elliott I, Inzhelevskaya T, Mockridge I, Penfold C, Wagner A, Glennie M, White A, Essex J, Pearson A, Cragg M, Tews I
RgGuinier 4.0 nm
Dmax 13.7 nm
VolumePorod 130 nm3

SASDKQ4 – EspK secretion protein - full length

ESX-1 secretion-associated protein EspK experimental SAS data
CORAL model
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl pH, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 20
Structural Analysis of the Partially Disordered Protein EspK from Mycobacterium Tuberculosis Crystals 11(1):18 (2020)
...Gao Y, Peters P, Ravelli R, Siliqi D
RgGuinier 7.2 nm
Dmax 3.3 nm
VolumePorod 330 nm3

SASDKR4 – EspK secretion protein - C-terminal domain

ESX-1 secretion-associated protein EspK experimental SAS data
GASBOR model
Sample: ESX-1 secretion-associated protein EspK monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl pH, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
Structural Analysis of the Partially Disordered Protein EspK from Mycobacterium Tuberculosis Crystals 11(1):18 (2020)
...Gao Y, Peters P, Ravelli R, Siliqi D
RgGuinier 2.2 nm
Dmax 8.4 nm
VolumePorod 52 nm3

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
...Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 23 nm3

SASDQ85 – Phosphofructokinase B (PfkB) from Mycobacterium marinum

Fructokinase, PfkB experimental SAS data
DAMMIF model
Sample: Fructokinase, PfkB monomer, 32 kDa Mycobacterium marinum (strain … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Dec 17
Structural analysis and functional study of phosphofructokinase B (PfkB) from Mycobacterium marinum Biochemical and Biophysical Research Communications (2021)
Gao B, Ji R, Li Z, Su X, Li H, Sun Y, Ji C, Gan J, Li J
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 58 nm3

SASDF95 – Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex

Uncharacterized proteinDNA Duplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
DNA Duplex dimer, 20 kDa DNA
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Sep 19
Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa Frontiers in Microbiology 10 (2020)
...Gao Z, Liu G, Geng Z, Dong Y, Zhang H
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 81 nm3

SASDQB5 – Elongation factor Tu from Mycobacterium tuberculosis

Elongation factor Tu experimental SAS data
DAMMIN model
Sample: Elongation factor Tu monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
...Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 3.5 nm
Dmax 9.0 nm
VolumePorod 94 nm3

SASDQC5 – Elongation factor Ts from Mycobacterium tuberculosis

Elongation factor Ts experimental SAS data
DAMMIN model
Sample: Elongation factor Ts monomer, 29 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
...Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 2.7 nm
Dmax 10.1 nm
VolumePorod 46 nm3

SASDQD5 – Elongation factor-Tu and Elongation factor-Ts complex from Mycobacterium tuberculosis

Elongation factor TuElongation factor Ts experimental SAS data
DAMMIN model
Sample: Elongation factor Tu monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
Elongation factor Ts monomer, 29 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jul 12
Structural insights of the elongation factor EF-Tu complexes in protein translation of Mycobacterium tuberculosis. Commun Biol 5(1):1052 (2022)
...Gao Y, Gao W, Li Y, Li Z, Qi Q, Lan X, Shen H, Gan J, Zhao G, Li J
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 111 nm3

SASDQE5 – Nucleoside triphosphate pyrophosphohydrolase (1–185)

Nucleoside triphosphate pyrophosphohydrolase experimental SAS data
DAMMIF model
Sample: Nucleoside triphosphate pyrophosphohydrolase dimer, 41 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2020 Dec 11
Structural analysis of the housecleaning nucleoside triphosphate pyrophosphohydrolase MazG from Mycobacterium tuberculosis Frontiers in Microbiology 14 (2023)
...Gao B, Chen A, Zhang Z, Wang S, Lv L, Zhao G, Li J
RgGuinier 2.7 nm
Dmax 8.4 nm
VolumePorod 64 nm3

SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)

Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain experimental SAS data
Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain Kratky plot
Sample: Lipid A export ATP-binding/permease protein MsbA - Nucleotide binding domain monomer, 27 kDa Escherichia coli protein
Buffer: 20 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.45 mM Mg2+-ATP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 8
Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering. Structure (2019)
...Gao Y, Monteiro DCF, Niebling S, Nitsche J, Veith K, Gräwert TW, Blanchet CE, Schroer MA, Huse N, Pearson AR, Svergun DI, Tidow H
RgGuinier 2.1 nm
Dmax 6.8 nm
VolumePorod 50 nm3

SASDBZ6 – Draxin

Draxin experimental SAS data
Draxin Rg histogram
Sample: Draxin monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 19
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC. Neuron 97(6):1261-1267.e4 (2018)
...Gao X, Mertens HDT, Svergun DI, Xiao J, Zhang Y, Wang JH, Meijers R
RgGuinier 4.2 nm
Dmax 15.0 nm
VolumePorod 87 nm3

SASDQ38 – Nucleoside triphosphate pyrophosphohydrolase (full-length)

Nucleoside triphosphate pyrophosphohydrolase experimental SAS data
DAMMIF model
Sample: Nucleoside triphosphate pyrophosphohydrolase dimer, 71 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Sep 4
Structural analysis of the housecleaning nucleoside triphosphate pyrophosphohydrolase MazG from Mycobacterium tuberculosis Frontiers in Microbiology 14 (2023)
...Gao B, Chen A, Zhang Z, Wang S, Lv L, Zhao G, Li J
RgGuinier 3.4 nm
Dmax 12.2 nm
VolumePorod 121 nm3

SASDPE8 – SMARCA2 bromodomain (isoform1):Protac (ACBI1):VCB (VHL-elongin C-elongin B)

Probable global transcription activator SNF2L2 (isoform 1)von Hippel-Lindau disease tumor suppressorElongin-BElongin-CACBI1 protac experimental SAS data
Probable global transcription activator SNF2L2 (isoform 1) von Hippel-Lindau disease tumor suppressor Elongin-B Elongin-C ACBI1 protac Kratky plot
Sample: Probable global transcription activator SNF2L2 (isoform 1) monomer, 16 kDa Homo sapiens protein
von Hippel-Lindau disease tumor suppressor monomer, 19 kDa Homo sapiens protein
Elongin-B monomer, 12 kDa Homo sapiens protein
Elongin-C monomer, 11 kDa Homo sapiens protein
ACBI1 protac monomer, 1 kDa
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2021 Aug 11
Predicting the structural basis of targeted protein degradation by integrating molecular dynamics simulations with structural mass spectrometry. Nat Commun 13(1):5884 (2022)
...Gao N, Jain S, Sparks S, Li Y, Vinitsky A, Zhang X, Razavi AM, Kolossváry I, Imbriglio J, Evdokimov A, Bergeron L, Zhou W, Adhikari J, Ruprecht B, Dickson A, Xu H, Sherman W, Izaguirre JA
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 83 nm3

SASDPF8 – SMARCA2 bromodomain (isoform2):Protac (ACBI1):VCB (VHL-elongin C-elongin B)

von Hippel-Lindau disease tumor suppressorElongin-BElongin-CACBI1 protacProbable global transcription activator SNF2L2 (isoform 2) experimental SAS data
von Hippel-Lindau disease tumor suppressor Elongin-B Elongin-C ACBI1 protac Probable global transcription activator SNF2L2 (isoform 2) Kratky plot
Sample: von Hippel-Lindau disease tumor suppressor monomer, 19 kDa Homo sapiens protein
Elongin-B monomer, 12 kDa Homo sapiens protein
Elongin-C monomer, 11 kDa Homo sapiens protein
ACBI1 protac monomer, 1 kDa
Probable global transcription activator SNF2L2 (isoform 2) monomer, 14 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, Département de Biochimie, Université de Montréal on 2021 Aug 11
Predicting the structural basis of targeted protein degradation by integrating molecular dynamics simulations with structural mass spectrometry. Nat Commun 13(1):5884 (2022)
...Gao N, Jain S, Sparks S, Li Y, Vinitsky A, Zhang X, Razavi AM, Kolossváry I, Imbriglio J, Evdokimov A, Bergeron L, Zhou W, Adhikari J, Ruprecht B, Dickson A, Xu H, Sherman W, Izaguirre JA
RgGuinier 3.2 nm
Dmax 11.1 nm
VolumePorod 75 nm3

SASDP89 – IgG Fc receptor CD64

High affinity immunoglobulin gamma Fc recombinant CD64 experimental SAS data
High affinity immunoglobulin gamma Fc recombinant CD64 Kratky plot
Sample: High affinity immunoglobulin gamma Fc recombinant CD64 monomer, 32 kDa synthetic construct protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Dec 9
The solution structure of the unbound IgG Fc receptor CD64 resembles its crystal structure: Implications for function. PLoS One 18(9):e0288351 (2023)
...Gao X, Gor J, Lu J, Sun PD, Perkins SJ
RgGuinier 3.4 nm
Dmax 12.3 nm
VolumePorod 30 nm3

SASDMD7 – ESX-1 secretion-associated protein EspB large construct

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.0 nm
Dmax 19.2 nm
VolumePorod 119 nm3

SASDME9 – 7SK stem-loop 1 with HEXIM Arginine Rich Motif

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNAProtein HEXIM1 experimental SAS data
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein HEXIM1 Kratky plot
Sample: Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA
Protein HEXIM1 monomer, 2 kDa Homo sapiens protein
Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA. Commun Biol 5(1):819 (2022)
...Gao M, Meagher JL, Smith JL, D'Souza VM
RgGuinier 2.2 nm
Dmax 8.2 nm
VolumePorod 26 nm3

SASDME7 – ESX-1 secretion-associated protein EspB medium construct

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 3.5 nm
Dmax 13.3 nm
VolumePorod 75 nm3

SASDMF9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype B (Isolate A18188)

Protein TatHomo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA experimental SAS data
Protein Tat Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Kratky plot
Sample: Protein Tat monomer, 2 kDa Human immunodeficiency virus … protein
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA
Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Sep 9
A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA. Commun Biol 5(1):819 (2022)
...Gao M, Meagher JL, Smith JL, D'Souza VM
RgGuinier 2.3 nm
Dmax 9.7 nm
VolumePorod 28 nm3

SASDQF4 – ESX-1 secretion-associated protein EspB

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB heptamer, 261 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.9 nm
Dmax 18.8 nm
VolumePorod 911 nm3

SASDMD9 – 7SK small nuclear RNA stem loop-1 bound to the arginine rich motif of HIV-1 Tat Group M, Subtype G

Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNAProtein Tat experimental SAS data
Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA Protein Tat Kratky plot
Sample: Homo sapiens RNA component of 7SK nuclear ribonucleoprotein (RN7SK), small nuclear RNA monomer, 18 kDa Homo sapiens RNA
Protein Tat monomer, 2 kDa Human immunodeficiency virus … protein
Buffer: 10 mM phosphate, 70 mM NaCl, 0.1 mM EDTA, pH: 5.6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 27
A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA. Commun Biol 5(1):819 (2022)
...Gao M, Meagher JL, Smith JL, D'Souza VM
RgGuinier 2.5 nm
Dmax 9.9 nm
VolumePorod 28 nm3

SASDMF7 – ESX-1 secretion-associated protein EspB small construct

ESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 21
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 56 nm3

SASDMG7 – ESX-1 secretion-associated protein EspB large construct bound to ESX-1 secretion-associated protein EspK — EspBL-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspK ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 48 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 5.0 nm
Dmax 18.3 nm
VolumePorod 128 nm3

SASDMH7 – ESX-1 secretion-associated protein EspB medium construct bound to ESX-1 secretion-associated protein EspK — EspBM-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
CORAL model
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 37 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Apr 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 4.3 nm
Dmax 15.7 nm
VolumePorod 100 nm3

SASDMJ7 – ESX-1 secretion-associated protein EspB small construct bound to ESX-1 secretion-associated protein EspK — EspBS-K complex

ESX-1 secretion-associated protein EspKESX-1 secretion-associated protein EspB experimental SAS data
ESX-1 secretion-associated protein EspK ESX-1 secretion-associated protein EspB Kratky plot
Sample: ESX-1 secretion-associated protein EspK monomer, 27 kDa Mycobacterium tuberculosis (strain … protein
ESX-1 secretion-associated protein EspB monomer, 30 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Sep 12
The crystal structure of the EspB-EspK virulence factor-chaperone complex suggests an additional type VII secretion mechanism in M. tuberculosis. J Biol Chem :102761 (2022)
...Gao Y, Menart I, Vinciauskaite V, Siliqi D, Peters PJ, McCarthy A, Ravelli RBG
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 113 nm3