Small Angle Scattering Biological Data Bank SASBDB


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60 hits found for Molodenskiy

SASDN92 – Apoform of glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p)

Glyceraldehyde-3-phosphate dehydrogenase 1 experimental SAS data

Sample:	Glyceraldehyde-3-phosphate dehydrogenase 1 tetramer, 142 kDa Kluyveromyces marxianus protein
Buffer:	150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 27

The Crystal and Solution Structures of Glyceraldehyde-3-phosphate Dehydrogenase Reveal Different Quaternary Structures Journal of Biological Chemistry 281(44):33433-33440 (2006)
Ferreira-da-Silva F, Pereira P, Gales L, Roessle M, Svergun D, Moradas-Ferreira P, Damas A

R_g^Guinier	4.2	nm
D_max	12.0	nm
Volume^Porod	234	nm³

SASDNA2 – Glyceraldehyde-3-phosphate dehydrogenase (apo-kmGAPDH1p) upon NAD+ binding

Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ experimental SAS data

Sample:	Glyceraldehyde-3-phosphate dehydrogenase 1 bound to NAD+ tetramer, 142 kDa Kluyveromyces marxianus protein
Buffer:	150 mM NaCl, 1 mM beta-mercaptoethanol, 1 mM EDTA, 10 mM TrisHCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 27

R_g^Guinier	3.7	nm
D_max	9.9	nm
Volume^Porod	202	nm³

SASDNB2 – Human NK inhibitory receptor IRp60 with an immunoglobulin-like fold

CMRF35-like molecule 8 experimental SAS data

Sample:	CMRF35-like molecule 8 monomer, 12 kDa Homo sapiens protein
Buffer:	MES buffer with 3mM DTT, pH: 5.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Apr 6

Molecular analysis and solution structure from small-angle X-ray scattering of the human natural killer inhibitory receptor IRp60 (CD300a) International Journal of Biological Macromolecules 40(3):193-200 (2007)
Dimasi N, Roessle M, Moran O, Candiano G, Svergun D, Biassoni R

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	22	nm³

SASDNC2 – NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12

Macrophage metalloelastase experimental SAS data

NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12 Rg histogram

Sample:	Macrophage metalloelastase monomer, 54 kDa Homo sapiens protein
Buffer:	20 mM Tris10 mM CaCl2, 0.3 M NaCl, 0.2 M AHA, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 2

Evidence of Reciprocal Reorientation of the Catalytic and Hemopexin-Like Domains of Full-Length MMP-12 Journal of the American Chemical Society 130(22):7011-7021 (2008)
Bertini I, Calderone V, Fragai M, Jaiswal R, Luchinat C, Melikian M, Mylonas E, Svergun D

R_g^Guinier	3.2	nm
D_max	11.0	nm
Volume^Porod	58	nm³

SASDND2 – The monomeric state of b-sandwich cupredoxin Plastocyanin (Pc)

Sample:	Plastocyanin monomer, 11 kDa Phormidium laminosum protein
Buffer:	pure water, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 May 7

Metal-Mediated Self-Assembly of a Î²-Sandwich Protein Chemistry - A European Journal 15(46):12672-12680 (2009)
Crowley P, Matias P, Khan A, Roessle M, Svergun D

R_g^Guinier	1.4	nm
D_max	4.5	nm
Volume^Porod	15	nm³

SASDNE2 – The dimeric state of b-sandwich cupredoxin Plastocyanin (Pc)

Sample:	Plastocyanin dimer, 23 kDa Phormidium laminosum protein
Buffer:	pure water, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 May 7

Metal-Mediated Self-Assembly of a Î²-Sandwich Protein Chemistry - A European Journal 15(46):12672-12680 (2009)
Crowley P, Matias P, Khan A, Roessle M, Svergun D

R_g^Guinier	2.3	nm
D_max	8.5	nm
Volume^Porod	28	nm³

SASDNN2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb2) at concentration c = 21 mg/ml

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG experimental SAS data

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG Kratky plot

Sample:	Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R

R_g^Guinier

3.0

SASDNP2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb5)

Sample:	Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	3.3	nm
D_max	13.0	nm

SASDNQ2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2) at concentration c = 25 mg/ml

Human hemoglobin conjugated with two copies of 5-kDa PEG experimental SAS data

Human hemoglobin conjugated with two copies of 5-kDa PEG Kratky plot

Sample:	Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier

2.4

SASDNR2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2)

Sample:	Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	2.8	nm
D_max	13.0	nm

SASDNS2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml

Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data

Sample:	Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	2.4	nm
D_max	13.0	nm

SASDNT2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 31.25 mg/ml

Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot

Sample:	Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier

2.2

SASDNU2 – Homodimerization of a membrane type 1 matrix metalloproteinase (MT1-MMP)

HemopexinHemopexin experimental SAS data

Sample:	Hemopexin monomer, 23 kDa Homo sapiens protein Hemopexin dimer, 46 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 10 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 25

The Dimer Interface of the Membrane Type 1 Matrix Metalloproteinase Hemopexin Domain Journal of Biological Chemistry 286(9):7587-7600 (2011)
Tochowicz A, Goettig P, Evans R, Visse R, Shitomi Y, Palmisano R, Ito N, Richter K, Maskos K, Franke D, Svergun D, Nagase H, Bode W, Itoh Y

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	48	nm³

SASDNV2 – The pro-convertase formed by human FB and cobra venom factor (CVF)

Cobra venom factor experimental SAS data

Sample:	Cobra venom factor monomer, 185 kDa Naja kaouthia protein
Buffer:	10 mM Tris 5 mM MgCl2 10 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 19

Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex The EMBO Journal 28(16):2469-2478 (2009)
Janssen B, Gomes L, Koning R, Svergun D, Koster A, Fritzinger D, Vogel C, Gros P

R_g^Guinier	4.6	nm
D_max	15.0	nm
Volume^Porod	384	nm³

SASDNW2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268)

Fab fragment in complex with small molecule hapten, crystal form-1 experimental SAS data

Sample:	Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
Buffer:	20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25

Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K

R_g^Guinier	3.0	nm
D_max	10.0	nm
Volume^Porod	71	nm³

SASDNX2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data

Sample:	Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer:	20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 27

R_g^Guinier	4.1	nm
D_max	16.0	nm
Volume^Porod	139	nm³

SASDNY2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) with TSC ligand excess

Sample:	Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein (1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer:	20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25

R_g^Guinier	3.6	nm
D_max	15.0	nm
Volume^Porod	107	nm³

SASDNZ2 – Tetrameric state of soluble endoglin receptor

Sample:	Endoglin tetramer, 243 kDa Homo sapiens protein
Buffer:	10 mM Tris–HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Oct 17

Structural and functional characterization of soluble endoglin receptor Biochemical and Biophysical Research Communications 383(4):386-391 (2009)
Le B, Franke D, Svergun D, Han T, Hwang H, Kim K

R_g^Guinier	7.6	nm
D_max	26.0	nm
Volume^Porod	434	nm³

SASDN23 – Homodimeric state of soluble endoglin receptor

Sample:	Endoglin dimer, 121 kDa Homo sapiens protein
Buffer:	10 mM Tris–HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Oct 17

R_g^Guinier	4.7	nm
D_max	17.0	nm
Volume^Porod	173	nm³

SASDN63 – Microtubule affinity regulating kinase (isoform MARK2, T208E point mutant)

Serine/threonine-protein kinase MARK2 experimental SAS data

Sample:	Serine/threonine-protein kinase MARK2 monomer, 36 kDa Homo sapiens protein
Buffer:	0.1 M Bis-Tris, 0.2 M ammonium citrate, 1mM DTT, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 16

Structural Variations in the Catalytic and Ubiquitin-associated Domains of Microtubule-associated Protein/Microtubule Affinity Regulating Kinase (MARK) 1 and MARK2 Journal of Biological Chemistry 281(37):27586-27599 (2006)
Marx A, Nugoor C, Müller J, Panneerselvam S, Timm T, Bilang M, Mylonas E, Svergun D, Mandelkow E, Mandelkow E

R_g^Guinier	2.4	nm
D_max	8.0	nm
Volume^Porod	61	nm³

SASDN73 – Microtubule affinity regulating kinase (isoform MARK2, wild type)

Sample:	Serine/threonine-protein kinase MARK2 monomer, 36 kDa Homo sapiens protein
Buffer:	0.1 M Bis-Tris, 0.2 M ammonium citrate, 1mM DTT, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 16

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	62	nm³

SASDN83 – Microtubule affinity regulating kinase (isoform MARK1)

Serine/threonine-protein kinase MARK1 experimental SAS data

Sample:	Serine/threonine-protein kinase MARK1 monomer, 37 kDa Homo sapiens protein
Buffer:	0.1 M Bis-Tris, 0.2 M ammonium citrate, 1mM DTT, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 16

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	64	nm³

SASDN93 – Dystrophiamyotonica kinase (DMPK)

Myotonin-protein kinase experimental SAS data

Sample:	Myotonin-protein kinase monomer, 92 kDa Homo sapiens protein
Buffer:	50 mM TrisHCl 50mM NaCl, 2.5 mM-mercaptoethanol, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Mar 18

Molecular insights into the self‐assembly mechanism of dystrophia myotonica kinase The FASEB Journal 20(8):1142-1151 (2006)
Garcia P, Ucurum Z, Bucher R, Svergun D, Huber T, Lustig A, Konarev P, Marino M, Mayans O

R_g^Guinier	3.9	nm
D_max	13.0	nm
Volume^Porod	173	nm³

SASDLF3 – Staphylococcal immunoglobulin-binding protein bound to complement component C3

Sample:	Protein A monomer, 28 kDa Staphylococcus aureus protein
Buffer:	10 mM HEPES, pH 7.2, 3 mM EDTA, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 12

A structural basis for Staphylococcal complement subversion: X-ray structure of the complement-binding domain of Staphylococcus aureus protein Sbi in complex with ligand C3d Molecular Immunology 48(4):452-462 (2011)
Clark E, Crennell S, Upadhyay A, Zozulya A, Mackay J, Svergun D, Bagby S, van den Elsen J

R_g^Guinier	4.6	nm
D_max	16.0	nm
Volume^Porod	81	nm³

SASDLG3 – Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data

Sample:	Regulatory protein E2 , 1037 kDa Human papillomavirus type … protein
Buffer:	50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 13

The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly FEBS Journal 279(5):713-723 (2012)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, Danson M, van den Elsen J

R_g^Guinier	8.8	nm
D_max	22.0	nm
Volume^Porod	2473	nm³

SASDLH3 – Truncated Thermoplasma E2 catalytic core

Sample:	Regulatory protein E2 trimer, 73 kDa Human papillomavirus type … protein
Buffer:	50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 27

Why are the 2-oxoacid dehydrogenase complexes so large? Generation of an active trimeric complex Biochemical Journal 463(3):405-412 (2014)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, van den Elsen J, Danson M

R_g^Guinier	3.1	nm
D_max	11.0	nm
Volume^Porod	149	nm³

SASDLJ3 – SH3 and multiple ankyrin repeat domains protein 3 (wild type)

SH3 and multiple ankyrin repeat domains protein 3 experimental SAS data

Sample:	SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer:	100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3

Autism associated SHANK3 missense point mutations impact conformational fluctuations and protein turnover at synapses. Elife 10 (2021)
...Molodenskiy D, Nia FH, Kreienkamp HJ, Svergun D, Kim E, Kostyukova AS, Kreutz MR, Mikhaylova M

R_g^Guinier	4.1	nm
D_max	14.0	nm
Volume^Porod	170	nm³

SASDLK3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (L68P)

Sample:	SH3 and multiple ankyrin repeat domains protein 3 monomer, 88 kDa Rattus norvegicus protein
Buffer:	100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3

R_g^Guinier	4.1	nm
D_max	14.8	nm
Volume^Porod	224	nm³

SASDLL3 – SH3 and multiple ankyrin repeat domains protein 3 with a point mutation (R12C)

Sample:	SH3 and multiple ankyrin repeat domains protein 3 monomer, 87 kDa Rattus norvegicus protein
Buffer:	100mM NaH2PO4, 100mM NaCl, 0.5mM DTT,, pH: 6.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Jun 3

R_g^Guinier	4.1	nm
D_max	13.8	nm
Volume^Porod	175	nm³

SASDMV3 – Sigma-Aldrich polystyrene nanospheres with a diameter of 100 nm

Nominal 100 nm diameter polystyrene spheres experimental SAS data

Sample:	Nominal 100 nm diameter polystyrene spheres monomer, 315312 kDa
Buffer:	Water, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 May 16

Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy

R_g^Guinier	33.0	nm
D_max	100.0	nm
Volume^Porod	520	nm³

SASDMW3 – Thermo Fisher polystyrene nanospheres with a diameter of 20 nm

Nominal 20 nm diameter polystyrene spheres experimental SAS data

Sample:	Nominal 20 nm diameter polystyrene spheres 0, 8 kDa
Buffer:	Water, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 May 16

Standard polystyrene nanospheres (concentration series data)
Dima Molodenskiy

R_g^Guinier	8.5	nm
D_max	25.0	nm
Volume^Porod	3	nm³

SASDKK4 – A-type ATP synthase

n-Dodecyl-β-D-MaltopyranosideA-type ATP synthaseMonoclonal Antibody Fragment experimental SAS data

Sample:	n-Dodecyl-β-D-Maltopyranoside , 77 kDa synthetic construct A-type ATP synthase monomer, 655 kDa Thermus thermophilus protein Monoclonal Antibody Fragment dimer, 33 kDa Homo sapiens protein
Buffer:	20 mM Tris/HCl, 100 mM sucrose, 100 mM NaCl, 2 mM MgCl2, 10% glycerol, 0.05% b-DDM, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2013 Mar 3

MPBuilder: A PyMOL Plugin for Building and Refinement of Solubilized Membrane Proteins Against Small Angle X-ray Scattering Data Journal of Molecular Biology :166888 (2021)
Molodenskiy D, Svergun D, Mertens H

R_g^Guinier	8.0	nm
D_max	27.0	nm
Volume^Porod	1549	nm³

SASDK36 – LC-18t - ssDNA aptamer specific to lung adenocarcinoma cancer cells

Lung adenocarcinoma aptamer, truncated version experimental SAS data

Sample:	Lung adenocarcinoma aptamer, truncated version monomer, 11 kDa Artificially synthesized DNA
Buffer:	Phosphate-buffered saline, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Oct 28

The Role of Small-Angle X-Ray Scattering and Molecular Simulations in 3D Structure Elucidation of a DNA Aptamer Against Lung Cancer Molecular Therapy - Nucleic Acids (2021)
...Molodenskiy D, Zabluda V, Veprintsev D, Sokolov A, Zukov R, Berezovski M, Tomilin F, Fedorov D, Alexeev Y, Kichkailo A

R_g^Guinier	2.1	nm
D_max	6.7	nm
Volume^Porod	16	nm³

SASDN77 – Truncated histone shaperone protein SET/TAF-Ib DC

SET nuclear proto-oncogene experimental SAS data

Sample:	SET nuclear proto-oncogene dimer, 53 kDa Homo sapiens protein
Buffer:	Sodium phosphate buffer, pH: 6.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 11

PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ Computational and Structural Biotechnology Journal (2022)
...Molodenskiy D, Díaz-Quintana A, Martinho M, Gerbaud G, González-Arzola K, Velázquez-Campoy A, Svergun D, Belle V, De la Rosa M, Díaz-Moreno I

R_g^Guinier	3.1	nm
D_max	9.7	nm
Volume^Porod	87	nm³

SASDN87 – Mixture 1:2 of truncated histone shaperone protein SET/TAF-Ib DC with cytochrome c

Cytochrome cSET nuclear proto-oncogene experimental SAS data

Sample:	Cytochrome c dimer, 24 kDa Homo sapiens protein SET nuclear proto-oncogene dimer, 53 kDa Homo sapiens protein
Buffer:	Sodium phosphate buffer, pH: 6.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2018 Nov 11

R_g^Guinier	4.2	nm
D_max	16.0	nm

SASDED8 – Poly-L-Glutamic Acid in Dimethyl Sulfoxide (DMSO)

Poly-L-Glutamic Acid experimental SAS data

Sample:	Poly-L-Glutamic Acid , 6 kDa
Buffer:	Dimethyl Sulfoxide (DMSO), pH:
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Dec 11

β2-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide. J Phys Chem B 122(50):11895-11905 (2018)
...Molodenskiy D, Wielgus-Kutrowska B, Johannessen C, Hernik-Magoń A, Tobias F, Bzowska A, Ścibisz G, Keiderling TA, Svergun D, Dzwolak W

R_g^Guinier	1.8	nm
D_max	7.8	nm
Volume^Porod	5	nm³

SASDPG8 – Solution of 0.1 wt% pentablock terpolymer (M18D170V209D170M18) in water at pH 2.6

M18D170V209D170M18 experimental SAS data

Sample:	M18D170V209D170M18 monomer, 1 kDa
Buffer:	H20, pH: 2.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 5

Highly Tunable Nanostructures in a Doubly pH‐Responsive Pentablock Terpolymer in Solution and in Thin Films Advanced Functional Materials 31(32):2102905 (2021)
...Molodenskiy D, Posselt D, Amenitsch H, Tsitsilianis C, Papadakis C

R_g^Guinier

8.7

SASDPH8 – Solution of 0.1 wt% pentablock terpolymer (M18D170V209D170M18) in water at pH 5.0

Sample:	M18D170V209D170M18 monomer, 1 kDa
Buffer:	H2O, pH: 5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 5

R_g^Guinier

8.2

SASDPJ8 – Solution of 0.1 wt% pentablock terpolymer (M18D170V209D170M18) in water at pH 6.9

Sample:	M18D170V209D170M18 monomer, 1 kDa
Buffer:	H2O, pH: 6.9
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 5

R_g^Guinier

5.4

SASDPK8 – Solution of 0.1 wt% pentablock terpolymer (M18D170V209D170M18) in water at pH 8.9

Sample:	M18D170V209D170M18 monomer, 1 kDa
Buffer:	H2O, pH: 8.9
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Dec 5

R_g^Guinier

7.3

SASDPL8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=1.25 mg/mL

PiPOx239-g-PnPrOx14 experimental SAS data

Sample:	PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer:	ethanol, pH: 7.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12

Rigid-to-Flexible Transition in a Molecular Brush in a Good Solvent at a Semidilute Concentration Langmuir 38(17):5226-5236 (2022)
...Molodenskiy D, Kohlbrecher J, Bushuev N, Gumerov R, Potemkin I, Jordan R, Papadakis C

R_g^Guinier	11.2	nm
D_max	37.6	nm

SASDPM8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=10 mg/mL

Sample:	PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer:	ethanol, pH: 7.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12

R_g^Guinier	8.6	nm
D_max	34.3	nm

SASDPN8 – Molecular brush (PiPOx239-g-PnPrOx14) in a good solvent at concentration c=46 mg/mL

Sample:	PiPOx239-g-PnPrOx14 monomer, 413 kDa
Buffer:	ethanol, pH: 7.3
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 12

R_g^Guinier	7.2	nm
D_max	18.5	nm

SASDMJ9 – Plasmid pET11d- 229E710 encoding C-terminally His6-tagged HCoV-229E Nsp7-10 polyprotein

Replicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity Journal of Virology 86(8):4444-4454 (2012)
Xiao Y, Ma Q, Restle T, Shang W, Svergun D, Ponnusamy R, Sczakiel G, Hilgenfeld R

R_g^Guinier

2.1

SASDMK9 – Nonstructural Proteins 7 and 8 of Feline Coronavirus (complex 1:2)

Replicase polyprotein 1aReplicase polyprotein 1a experimental SAS data

Sample:	Replicase polyprotein 1a dimer, 19 kDa Severe acute respiratory … protein Replicase polyprotein 1a monomer, 22 kDa Severe acute respiratory … protein
Buffer:	10 mM Tris-HCl, 200 mM NaCl, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Apr 2

R_g^Guinier

3.0

SASDML9 – Structure of bifunctional protease-helicase NS3/4A domain assembly in solution

Genome polyprotein experimental SAS data

Sample:	Genome polyprotein dimer, 137 kDa Hepatitis C virus … protein
Buffer:	25 mM Tris, 1 M NaCl, 10% glycerol, 1 mM TCEP, 0.1% β-octyl glucoside, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 12

A macrocyclic HCV NS3/4A protease inhibitor interacts with protease and helicase residues in the complex with its full-length target Proceedings of the National Academy of Sciences 108(52):21052-21056 (2011)
Schiering N, D'Arcy A, Villard F, Simic O, Kamke M, Monnet G, Hassiepen U, Svergun D, Pulfer R, Eder J, Raman P, Bodendorf U

R_g^Guinier

3.9

SASDMM9 – Response Regulator RocR from Pseudomonas aeruginosa

Sample:	RocR tetramer, 171 kDa Pseudomonas aeruginosa (strain … protein
Buffer:	50 mM Tris–HCl, 250 mM NaCl, 10 mM imidazole, 5% glycerol, 0.5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 9

Structural Insights into the Regulatory Mechanism of the Response Regulator RocR from Pseudomonas aeruginosa in Cyclic Di-GMP Signaling Journal of Bacteriology 194(18):4837-4846 (2012)
Chen M, Kotaka M, Vonrhein C, Bricogne G, Rao F, Chuah M, Svergun D, Schneider G, Liang Z, Lescar J

R_g^Guinier	3.7	nm
D_max	11.0	nm

SASDMN9 – Conformational characterization of A77-78 fibronectin type III tandem

Sample:	Titin monomer, 22 kDa Homo sapiens protein
Buffer:	100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3

The Structure of the FnIII Tandem A77-A78 Points to a Periodically Conserved Architecture in the Myosin-Binding Region of Titin Journal of Molecular Biology 401(5):843-853 (2010)
Bucher R, Svergun D, Muhle-Goll C, Mayans O

R_g^Guinier	2.5	nm
D_max	90.0	nm
Volume^Porod	21	nm³

SASDMP9 – Conformational characterization of A80-82 fibronectin type III tandem

Sample:	Titin monomer, 32 kDa Homo sapiens protein
Buffer:	100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 3

R_g^Guinier	3.7	nm
D_max	130.0	nm
Volume^Porod	39	nm³

SASDMQ9 – Conformational characterization of A84-86 fibronectin type III tandem

Sample:	Titin monomer, 32 kDa Homo sapiens protein
Buffer:	100 mM NaCl, 50 mM Tris-HCl, 2mM DTT, pH: 7.2
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Oct 6

R_g^Guinier	3.7	nm
D_max	14.0	nm

SASDJE6 – Metallothionein (MT) 2 protein from the plant Cicer arietinum

Metallothionein-like protein 2 experimental SAS data

Metallothionein (MT) 2 protein from the plant Cicer arietinum Rg histogram

Sample:	Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	1.9	nm
D_max	7.3	nm
Volume^Porod	5	nm³

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
...Molodenskiy D, Blanchet C, Svergun D, Jeffries C

R_g^Guinier	1.8	nm
D_max	5.1	nm
Volume^Porod	37	nm³

SASDJF6 – Apo-full-length protein titrated with Cd(II) ions

Apo-full-length protein titrated with Cd(II) ions Rg histogram

Sample:	Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl + 1mM TCEP (apoMT), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	13	nm³

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	98	nm³

SASDJG6 – The separated apo-linker region peptide

The separated apo-linker region peptide from the plant Cicer arietinum experimental SAS data

The separated apo-linker region peptide Rg histogram

Sample:	The separated apo-linker region peptide from the plant Cicer arietinum monomer, 4 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	1.4	nm
D_max	6.5	nm
Volume^Porod	1	nm³

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	211	nm³

SASDJH6 – The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site

A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site experimental SAS data

The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site Rg histogram

Sample:	A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site monomer, 5 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	1.9	nm
D_max	6.3	nm

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data

Sample:	Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	3.3	nm
D_max	9.3	nm
Volume^Porod	201	nm³

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data

Sample:	Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	5.4	nm
D_max	12.5	nm
Volume^Porod	679	nm³

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23

R_g^Guinier	2.8	nm
D_max	8.3	nm
Volume^Porod	95	nm³

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