Small Angle Scattering Biological Data Bank SASBDB


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46 hits found for Calmodulin

SASDB52 – Death associated protein kinase (wild-type)

Death associated protein kinase wild-type experimental SAS data

Sample:	Death associated protein kinase wild-type , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

...Calmodulin Binding to Two Distinct Sites. Structure 24(6):851-61 (2016)
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M

R_g^Guinier	2.7	nm
D_max	8.1	nm
Volume^Porod	101	nm³

SASDB62 – Death associated protein kinase (D220K mutant)

Death associated protein kinase (D220K mutant) experimental SAS data

Sample:	Death associated protein kinase (D220K mutant) , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

R_g^Guinier	2.5	nm
D_max	9.0	nm
Volume^Porod	89	nm³

SASDB72 – Death associated protein kinase (Basic Loop mutant)

Death associated protein kinase (Basic Loop mutant) experimental SAS data

Sample:	Death associated protein kinase (Basic Loop mutant) , 37 kDa Homo sapiens protein
Buffer:	50 mM HEPES 250 mM NaCl 5mM CaCl2 0.25 mM TCEP 5% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 18

R_g^Guinier	2.4	nm
D_max	7.5	nm
Volume^Porod	78	nm³

SASDCQ2 – ...calmodulin - Xenopus laevis

4Ca2+-calmodulin - Xenopus laevis Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein
Buffer:	25 mM MOPS, 250 mM NaCl, 50 mM KCl, 2 mM TCEP, 0.1% NaN3, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Mar 9

2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update. Acta Crystallogr D Struct Biol 73(Pt 9):710-728 (2017)
Trewhella J, Duff AP, Durand D, Gabel F, Guss JM, Hendrickson WA, Hura GL, Jacques DA, Kirby NM, Kwan AH, Pérez J, Pollack L, Ryan TM, Sali A, Schneidman-Duhovny D, Schwede T, Svergun DI, Sugiyama M, ...

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	25	nm³

SASDKR3 – ...calmodulin bound to the HIV-1 MA protein

Calmodulin-1calcium ionsGag-Pol polyprotein experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Xenopus laevis protein calcium ions tetramer, 0 kDa Gag-Pol polyprotein monomer, 15 kDa Human immunodeficiency virus … protein
Buffer:	50 mM MOPS, 5 mM CaCl2, 2 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at Bruker Nanostar, Australian Nuclear Science and Technology Organisation on 2010 Jun 23

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release. Biophys J 103(3):541-549 (2012)
Taylor JE, Chow JYH, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J

R_g^Guinier	3.0	nm
D_max	11.0	nm
Volume^Porod	55	nm³

SASDNX3 – ...Calmodulin, including structural models

Calcium-bound Calmodulin, including structural models Rg histogram

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

...calmodulin upon interaction with the antagonist calmidazolium. BMC Biol 20(1):176 (2022)
Léger C, Pitard I, Sadi M, Carvalho N, Brier S, Mechaly A, Raoux-Barbot D, Davi M, Hoos S, Weber P, Vachette P, Durand D, Haouz A, Guijarro JI, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.2	nm
Volume^Porod	33	nm³

SASDNY3 – ...Calmodulin complexed with Calmidazolium

Calmodulin-1Calmidazolium experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Calmidazolium monomer, 1 kDa
Buffer:	20 mM Hepes, 150 mM NaCl, 2 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2020 Nov 15

R_g^Guinier	1.7	nm
D_max	5.2	nm
Volume^Porod	30	nm³

SASDJ64 – ...Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

...Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A

R_g^Guinier	2.2	nm
D_max	7.3	nm
Volume^Porod	27	nm³

SASDJ74 – ...calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer:	20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2016 Sep 24

R_g^Guinier	2.0	nm
D_max	7.0	nm
Volume^Porod	28	nm³

SASDAN4 – Calmodulin:peptide complex

CalmodulinC-terminal region of human myelin basic protein experimental SAS data

Sample:	Calmodulin monomer, 17 kDa Homo sapiens protein C-terminal region of human myelin basic protein monomer, 2 kDa Homo sapiens protein
Buffer:	25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 28

...calmodulin: analysis of complex formation and solution structure. BMC Struct Biol 8:10 (2008)
Majava V, Petoukhov MV, Hayashi N, Pirilä P, Svergun DI, Kursula P

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	36	nm³

SASDAQ4 – 6:3 complex of GAD:CaM

CalmodulinGlutamate decarboxylase experimental SAS data

Sample:	Calmodulin monomer, 17 kDa Homo sapiens protein Glutamate decarboxylase hexamer, 340 kDa Petunia x hybrida protein
Buffer:	50 mM HEPES 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2004 Nov 9

...calmodulin-mediated regulation in plant glutamate decarboxylase. J Mol Biol 392(2):334-51 (2009)
Gut H, Dominici P, Pilati S, Astegno A, Petoukhov MV, Svergun DI, Grütter MG, Capitani G

R_g^Guinier	5.9	nm
D_max	23.0	nm
Volume^Porod	630	nm³

SASDUQ4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3

Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S

R_g^Guinier	4.5	nm
D_max	15.2	nm
Volume^Porod	367	nm³

SASDUR4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19

R_g^Guinier	4.4	nm
D_max	14.9	nm
Volume^Porod	351	nm³

SASDES4 – ACA8 in stealth nanodisc (SANS, 100% D2O)

Membrane scaffold protein 1D1 (deuterated, 75%)1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)Calcium-transporting ATPase 8, plasma membrane-type experimental SAS data

Sample:	Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Buffer:	30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment:	SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28

...calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H

R_g^Guinier	4.0	nm
D_max	13.0	nm
Volume^Porod	202	nm³

SASDUS4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (i)

Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30

R_g^Guinier	4.8	nm
D_max	16.0	nm
Volume^Porod	396	nm³

SASDET4 – ...Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O)

ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O) Rg histogram

Sample:	Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 (deuterated 75%) monomer, Arabidopsis thaliana protein
Buffer:	30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment:	SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28

...calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H

R_g^Guinier	4.3	nm
D_max	15.0	nm
Volume^Porod	217	nm³

SASDUT4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii)

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30

R_g^Guinier	5.5	nm
D_max	27.0	nm

SASDEU4 – ...Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)

Sample:	Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer:	30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment:	SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28

...calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H

R_g^Guinier	4.8	nm
D_max	18.0	nm
Volume^Porod	297	nm³

SASDUU4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage)

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 31

R_g^Guinier	8.8	nm
D_max	37.0	nm

SASDEV4 – ACA8 protein (apo) in stealth nanodisc

Sample:	Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein
Buffer:	30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8

...calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H

R_g^Guinier	5.3	nm
D_max	20.0	nm
Volume^Porod	626	nm³

SASDUV4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iii)

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30

R_g^Guinier	4.5	nm
D_max	17.0	nm
Volume^Porod	556	nm³

SASDEW4 – ...Calmodulin (hydrogenated) in stealth nanodisc

Sample:	Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl) , 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 monomer, Arabidopsis thaliana protein
Buffer:	30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8

...calmodulin. Commun Biol 1:206 (2018)
Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H

R_g^Guinier	5.9	nm
D_max	22.0	nm
Volume^Porod	805	nm³

SASDUW4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (iv)

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17

R_g^Guinier	15.6	nm
D_max	90.0	nm

SASDUX4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha5-hydroxydiclofenac experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha 5-hydroxydiclofenac Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 5-hydroxydiclofenac monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3

R_g^Guinier	4.5	nm
D_max	17.5	nm
Volume^Porod	372	nm³

SASDUY4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with HOCPCA

Calcium/calmodulin-dependent protein kinase type II subunit alpha3-hydroxycyclopent-1-enecarboxylic acid experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha 3-hydroxycyclopent-1-enecarboxylic acid Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein 3-hydroxycyclopent-1-enecarboxylic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 1 mM HOCPCA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19

R_g^Guinier	4.1	nm
D_max	12.7	nm
Volume^Porod	310	nm³

SASDUZ4 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17

R_g^Guinier	4.7	nm
D_max	16.5	nm
Volume^Porod	358	nm³

SASDU25 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Mar 17

R_g^Guinier	4.4	nm
D_max	15.0	nm
Volume^Porod	334	nm³

SASDU35 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) W403L hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L)5-hydroxydiclofenac experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha (W403L) 5-hydroxydiclofenac Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (W403L) dodecamer, 185 kDa Homo sapiens protein 5-hydroxydiclofenac monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L, Département de Biochimie, Université de Montréal on 2022 Dec 15

R_g^Guinier	4.4	nm
D_max	15.0	nm
Volume^Porod	335	nm³

SASDU45 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) experimental SAS data

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3

R_g^Guinier	4.7	nm
D_max	18.0	nm
Volume^Porod	329	nm³

SASDU55 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19

R_g^Guinier	4.5	nm
D_max	14.5	nm
Volume^Porod	313	nm³

SASDU65 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with PIPA

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30

R_g^Guinier	4.7	nm
D_max	20.0	nm
Volume^Porod	375	nm³

SASDU75 – ...calmodulin-dependent protein kinase type II alpha (CaMKIIα) 6x hub domain with 5-HDC

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M)5-hydroxydiclofenac experimental SAS data

Calcium/calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 5-hydroxydiclofenac Kratky plot

Sample:	...calmodulin-dependent protein kinase type II subunit alpha (T354N, E355Q, T412N, I414M, I464H, F467M) 14-mer, 217 kDa Homo sapiens protein 5-hydroxydiclofenac monomer, 0 kDa
Buffer:	20 mM MES, 150 mM NaCl, 1 mM DTT, 100 µM 5-HDC, pH: 6
Experiment:	SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19

R_g^Guinier	4.7	nm
D_max	18.5	nm
Volume^Porod	366	nm³

SASDPV6 – Calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

...calmodulin is coupled to transient unfolding of the third FF domain of Prp40A. Protein Sci 32(4):e4606 (2023)
Díaz Casas A, Cordoba JJ, Ferrer BJ, Balakrishnan S, Wurm JE, Pastrana-Ríos B, Chazin WJ

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	38	nm³

SASDPW6 – FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)

Pre-mRNA-processing factor 40 homolog A experimental SAS data

Sample:	Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

R_g^Guinier	1.6	nm
D_max	7.2	nm
Volume^Porod	13	nm³

SASDPX6 – Calmodulin bound to the FF3 domain of pre-mRNA-processing factor 40 homolog A (Prp40A)

Calmodulin-1Pre-mRNA-processing factor 40 homolog A experimental SAS data

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein Pre-mRNA-processing factor 40 homolog A monomer, 9 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 100 mM NaCl, 2 mM CaCl2, 1 mM TCEP, pH: 7.4
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Sep 23

R_g^Guinier	2.4	nm
D_max	8.7	nm
Volume^Porod	29	nm³

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDCL9 – ...calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

R_g^Guinier	2.9	nm
D_max	9.8	nm
Volume^Porod	78	nm³

SASDPE4 – P2X7 ballast domain

P2X purinoceptor 7 experimental SAS data

Sample:	P2X purinoceptor 7 trimer, 74 kDa Homo sapiens protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4

...calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H

R_g^Guinier	3.1	nm
D_max	10.5	nm
Volume^Porod	94	nm³

SASDPD4 – ...calmodulin

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4

...calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H

R_g^Guinier	2.1	nm
D_max	6.9	nm
Volume^Porod	23	nm³

SASDSR8 – ...calmodulin-dependent protein kinase kinase 1 (CaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot

Sample:	...calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17

14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
Petrvalska O, Honzejkova K, Koupilova N, Herman P, Obsilova V, Obsil T

R_g^Guinier	3.2	nm
D_max	10.7	nm
Volume^Porod	114	nm³

SASDPF4 – ...calmodulin

Calmodulin-1P2X purinoceptor 7 experimental SAS data

Calmodulin-1 P2X purinoceptor 7 Kratky plot

Sample:	Calmodulin-1 monomer, 17 kDa Homo sapiens protein P2X purinoceptor 7 monomer, 25 kDa Homo sapiens protein
Buffer:	20 mM HEPES pH 7.5, 150 mM NaCl, 5 mM CaCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Jun 4

...calmodulin and GDP bind to the soluble P2X7 ballast domain. J Biol Chem :102495 (2022)
Sander S, Müller I, Alai MG, Nicke A, Tidow H

R_g^Guinier	3.2	nm
D_max	13.5	nm
Volume^Porod	73	nm³

SASDSS8 – ...calmodulin-dependent protein kinase 1 (pCaMKK1)

Sample:	...calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17

R_g^Guinier	3.0	nm
D_max	10.5	nm
Volume^Porod	97	nm³

SASDSQ7 – ...calmodulin-dependent protein kinase kinase 2 (CaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data

Sample:	...calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3

R_g^Guinier	3.2	nm
D_max	13.3	nm
Volume^Porod	91	nm³

SASDST8 – ...calmodulin-dependent protein kinase 2 (pCaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 Kratky plot

Sample:	...calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17

R_g^Guinier	3.0	nm
D_max	12.0	nm
Volume^Porod	87	nm³

SASDS46 – ...calmodulin-dependent protein kinase kinase 1 (CaMKK1) bound to 14-3-3 protein gamma

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L)14-3-3 protein gamma experimental SAS data

Sample:	...calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23

R_g^Guinier	3.3	nm
D_max	10.8	nm
Volume^Porod	152	nm³

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma

14-3-3 protein gammaCalcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data

Sample:	14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein ...calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23

R_g^Guinier	3.7	nm
D_max	13.2	nm
Volume^Porod	157	nm³

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