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84 hits found for Lin

SASDKF2 – Mixed-sequence DNA dimer (25 base pair DNA)

Mixed-sequence 25 base-paired DNA experimental SAS data
Mixed-sequence 25 base-paired DNA Kratky plot
Sample: Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 17 nm3

SASDHG2 – 12 base-paired RNA double helix (RNA12) with 30 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 30 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDKG2 – AT-sequence DNA dimer (25 base pair DNA)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
He W, Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 16 nm3

SASDHH2 – 12 base-paired RNA double helix (RNA12) with 50 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 50 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDHJ2 – 12 base-paired RNA double helix (RNA12) with 100 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 100 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.5 nm

SASDHK2 – 12 base-paired RNA double helix (RNA12) with 200 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 200 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHL2 – 12 base-paired RNA double helix (RNA12) with 500 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 500 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHM2 – 12 base-paired RNA double helix (RNA12) with 0.25 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 0.25 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDHN2 – 12 base-paired RNA double helix (RNA12) with 1 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 1.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 5.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDHQ2 – 25 base-paired RNA double helix (RNA25) with 100 mM NaCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.6 nm

SASDHS2 – 25 base-paired RNA double helix (RNA25) with 400 mM KCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.3 nm

SASDHT2 – 25 base-paired DNA double helix (DNA25) with 400 mM KCl - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDHU2 – 25 base-paired DNA double helix (DNA25) with 0.5 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 0.5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 2.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.5 nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data
A dENE + Poly(A)30 Kratky plot
Sample: A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 13.3 nm

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

B dENE experimental SAS data
B dENE Kratky plot
Sample: B dENE monomer, 24 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.8 nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data
B dENE + Poly(A)30 Kratky plot
Sample: B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

C dENE experimental SAS data
C dENE Kratky plot
Sample: C dENE monomer, 29 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.4 nm
Dmax 12.4 nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Xtal dENE experimental SAS data
Xtal dENE Kratky plot
Sample: Xtal dENE monomer, 28 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.1 nm
Dmax 11.0 nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDKK3 – SEC-SAXS of Presequence Protease (PreP)

Presequence protease, mitochondrial experimental SAS data
Presequence protease, mitochondrial Kratky plot
Sample: Presequence protease, mitochondrial monomer, 115 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 7.7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 4
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition Nature Communications 13(1) (2022)
...Lin King J, Pan M, Liu C, Koehler C, Zhao M, Potter C, Carragher B, Li S, Tang W
RgGuinier 3.2 nm
Dmax 9.1 nm
VolumePorod 170 nm3

SASDKL3 – SEC-SAXS of Presequence Protease (PreP) with inhibitor MitoBloCK-60 (MB-60)

Presequence protease, mitochondrial experimental SAS data
Presequence protease, mitochondrial Kratky plot
Sample: Presequence protease, mitochondrial monomer, 115 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 7.7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 7
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition Nature Communications 13(1) (2022)
...Lin King J, Pan M, Liu C, Koehler C, Zhao M, Potter C, Carragher B, Li S, Tang W
RgGuinier 3.1 nm
Dmax 9.1 nm
VolumePorod 165 nm3

SASDKM3 – SEC-SAXS of Presequence Protease (PreP) with pre-sequence of citrate synthase (1-27)

Presequence protease, mitochondrialCitrate synthase, mitochondrial experimental SAS data
Presequence protease, mitochondrial Citrate synthase, mitochondrial Kratky plot
Sample: Presequence protease, mitochondrial monomer, 115 kDa Homo sapiens protein
Citrate synthase, mitochondrial monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl , 20 mM EDTA, pH: 7.7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 4
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition Nature Communications 13(1) (2022)
...Lin King J, Pan M, Liu C, Koehler C, Zhao M, Potter C, Carragher B, Li S, Tang W
RgGuinier 3.1 nm
Dmax 8.7 nm
VolumePorod 165 nm3

SASDKN3 – SEC-SAXS of Presequence Protease (PreP) with Amyloid beta precursor protein (1-40)

Presequence protease, mitochondrialAmyloid-beta precursor protein experimental SAS data
Presequence protease, mitochondrial Amyloid-beta precursor protein Kratky plot
Sample: Presequence protease, mitochondrial monomer, 115 kDa Homo sapiens protein
Amyloid-beta precursor protein monomer, 4 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl , 20 mM EDTA, pH: 7.7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 7
Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition Nature Communications 13(1) (2022)
...Lin King J, Pan M, Liu C, Koehler C, Zhao M, Potter C, Carragher B, Li S, Tang W
RgGuinier 3.0 nm
Dmax 8.5 nm
VolumePorod 175 nm3

SASDVW3 – Lipoprotein NlpI dimer bound to two asymmetrical murein DD-endopeptidase mMepS dimers at pH 7.0

Lipoprotein NlpIMurein DD-endopeptidase MepS/Murein LD-carboxypeptidase experimental SAS data
PYMOL model
Sample: Lipoprotein NlpI dimer, 63 kDa Escherichia coli (strain … protein
Murein DD-endopeptidase MepS/Murein LD-carboxypeptidase tetramer, 73 kDa Escherichia coli (strain … protein
Buffer: 0.2 M Na/K phosphate pH 7.0, 2 mM 2-mercaptoethanol, pH: 7
Experiment: SAXS data collected at TPS13A, NSRRC on 2023 Sep 7
Structural basis for recruitment of peptidoglycan endopeptidase MepS by lipoprotein NlpI. Nat Commun 15(1):5461 (2024)
...Lin TS, Yeh YQ, Fan YS, Wang SW, Tseng HC, Huang SJ, Chang YY, Jeng US, Chang CI, Tzeng SR
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 123 nm3

SASDKG5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: ...lino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKH5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKJ5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: ...lino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKK5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKL5 – RNA Short Tetraloop Hairpin Triplex

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.0 nm
Dmax 6.3 nm

SASDVL5 – Small EDRK-rich factor 1 (SERF1a)

Isoform Short of Small EDRK-rich factor 1 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Mar 11
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 9 nm3

SASDKM5 – Metastasis-Associated Lung Adenocarcinoma Transcript 1

Metastasis-Associated Lung Adenocarcinoma Transcript 1 experimental SAS data
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Kratky plot
Sample: Metastasis-Associated Lung Adenocarcinoma Transcript 1 monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 μM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.3 nm
Dmax 7.9 nm

SASDVM5 – HTT-3 peptide

HTT3 experimental SAS data
ROSETTA model
Sample: HTT3 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 May 20
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 2.0 nm

SASDKN5 – 30nt Single-stranded RNA

30nt Single-stranded RNA experimental SAS data
30nt Single-stranded RNA Kratky plot
Sample: 30nt Single-stranded RNA monomer, 10 kDa RNA
Buffer: ...lino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
...Lin Chen
RgGuinier 2.2 nm
Dmax 8.2 nm

SASDVN5 – NT17 peptide

NT17 experimental SAS data
ROSETTA model
Sample: NT17 monomer, 2 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Oct 21
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 1.2 nm
Dmax 4.0 nm
VolumePorod 5 nm3

SASDKP5 – RNA Short Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDVP5 – HTT0 peptide

HTT0 experimental SAS data
ROSETTA model
Sample: HTT0 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2023 Nov 10
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 1.5 nm
Dmax 6.0 nm
VolumePorod 4 nm3

SASDKQ5 – RNA Short Tetraloop Hairpin Duplex in 200 mM KCl

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.0 nm
Dmax 6.8 nm

SASDVQ5 – HTT1 peptide

HTT1 experimental SAS data
HTT1 Kratky plot
Sample: HTT1 dimer, 8 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Feb 3
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 18 nm3

SASDKR5 – RNA Short Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.1 nm
Dmax 7.8 nm

SASDVR5 – Small EDRK-rich factor 1 (SERF1a) bound to NT17 peptide

Isoform Short of Small EDRK-rich factor 1NT17 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
NT17 dimer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 Oct 21
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 2.3 nm

SASDKS5 – RNA Short Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.3 nm

SASDVS5 – Small EDRK-rich factor 1 (SERF1a) bound to HTT-3 peptide

Isoform Short of Small EDRK-rich factor 1HTT3 experimental SAS data
ROSETTA model
Sample: Isoform Short of Small EDRK-rich factor 1 monomer, 7 kDa Homo sapiens protein
HTT3 monomer, 4 kDa synthetic construct protein
Buffer: Sodium phosphate buffer, pH: 7.4
Experiment: SAXS data collected at TPS13A, NSRRC on 2021 May 20
Binding structures of SERF1a with NT17-polyQ peptides of huntingtin exon 1 revealed by SEC-SWAXS, NMR and molecular simulation. IUCrJ (2024)
Lin TC, Shih O, Tsai TY, Yeh YQ, Liao KF, Mansel BW, Shiu YJ, Chang CF, Su AC, Chen YR, Jeng US
RgGuinier 2.3 nm
Dmax 7.2 nm
VolumePorod 9 nm3

SASDKT5 – RNA Short Tetraloop Hairpin Triplex in 200 mM KCl

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 1.9 nm
Dmax 6.6 nm

SASDKU5 – RNA Short Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.2 nm
Dmax 10.2 nm

SASDKV5 – RNA Long Tetraloop Hairpin Duplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 9.8 nm

SASDKW5 – RNA Long Tetraloop Hairpin Duplex in 200 mM KCl

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.0 nm

SASDKX5 – RNA Long Tetraloop Hairpin Duplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Duplex experimental SAS data
RNA Long Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Duplex monomer, 21 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 10.3 nm

SASDKY5 – RNA Long Tetraloop Hairpin Triplex in 200 mM NaCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM NaCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.7 nm
Dmax 10.3 nm

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDK26 – RNA Long Tetraloop Hairpin Triplex in 5 mM MgCl2

RNA Long Tetraloop Hairpin Triplex experimental SAS data
RNA Long Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Long Tetraloop Hairpin Triplex monomer, 28 kDa RNA
Buffer: 5 mM MgCl2, 20 mM Na-MES, 50 μM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
RNA triplex structures revealed by WAXS-driven MD simulations (2022)
Chen Y, He W, Kirmizialtin S, Pollack L
RgGuinier 2.9 nm
Dmax 18.2 nm

SASDNF6 – full-length MERS CoV N -protein complexed with 5-propoxy-1H-indole (P4-1 compound)

Nucleoprotein5-(Propoxy)-1H-indole experimental SAS data
CORAL model
Sample: Nucleoprotein dodecamer, 548 kDa Middle East respiratory … protein
5-(Propoxy)-1H-indole dodecamer, 2 kDa
Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 22
Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation Frontiers in Molecular Biosciences 9 (2022)
...Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M
RgGuinier 6.4 nm
Dmax 22.0 nm
VolumePorod 896 nm3

SASDNG6 – full-length MERS CoV N-protein complexed with 5-Isopropoxy-1H-indole (P4-2 compound)

Nucleoprotein5-Isopropoxy-1H-indole experimental SAS data
CORAL model
Sample: Nucleoprotein dodecamer, 548 kDa Middle East respiratory … protein
5-Isopropoxy-1H-indole dodecamer, 2 kDa
Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 22
Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation Frontiers in Molecular Biosciences 9 (2022)
...Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M
RgGuinier 6.4 nm
Dmax 22.2 nm
VolumePorod 911 nm3

SASDNH6 – full-length MERS CoV N-protein complexed with 5-(2-fluoroethoxy)-1H-indole (P4-3 compound)

Nucleoprotein5-(2-fluoroethoxy)-1H-indole experimental SAS data
CORAL model
Sample: Nucleoprotein tetramer, 183 kDa Middle East respiratory … protein
5-(2-fluoroethoxy)-1H-indole tetramer, 1 kDa
Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 26
Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation Frontiers in Molecular Biosciences 9 (2022)
...Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M
RgGuinier 5.7 nm
Dmax 19.1 nm
VolumePorod 477 nm3

SASDNJ6 – full-length MERS CoV N-protein complexed with 5-(2-methoxyethoxy)-1H-indole (P4-4 compound)

Nucleoprotein5-(2-methoxyethoxy)-1H-indole experimental SAS data
CORAL model
Sample: Nucleoprotein tetramer, 183 kDa Middle East respiratory … protein
5-(2-methoxyethoxy)-1H-indole tetramer, 1 kDa
Buffer: 50 mM Tris-HCl, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Nov 26
Targeting the N-Terminus Domain of the Coronavirus Nucleocapsid Protein Induces Abnormal Oligomerization via Allosteric Modulation Frontiers in Molecular Biosciences 9 (2022)
...Lin S, Hong J, Chen Y, Jeng U, Luo S, Hou M
RgGuinier 6.0 nm
Dmax 18.5 nm
VolumePorod 500 nm3

SASDRB9 – Bruton's Tyrosine Kinase - full-length

Tyrosine-protein kinase BTK experimental SAS data
DAMMIF model
Sample: Tyrosine-protein kinase BTK monomer, 77 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9
Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states. Elife 12 (2024)
Lin DY, Kueffer LE, Juneja P, Wales TE, Engen JR, Andreotti AH
RgGuinier 4.1 nm
Dmax 19.1 nm
VolumePorod 118 nm3

SASDRC9 – Bruton's Tyrosine Kinase - full-length with A384P, S386P, T387P, A388P and L390F mutations

Tyrosine-protein kinase BTK (A384P, S386P, T387P, A388P, L390F) experimental SAS data
DAMMIF model
Sample: Tyrosine-protein kinase BTK (A384P, S386P, T387P, A388P, L390F) monomer, 77 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9
Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states. Elife 12 (2024)
Lin DY, Kueffer LE, Juneja P, Wales TE, Engen JR, Andreotti AH
RgGuinier 3.9 nm
Dmax 15.2 nm
VolumePorod 107 nm3

SASDRD9 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain

Tyrosine-protein kinase BTK (SH3-SH2-kinase domains) experimental SAS data
DAMMIF model
Sample: Tyrosine-protein kinase BTK (SH3-SH2-kinase domains) monomer, 52 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9
Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states. Elife 12 (2024)
Lin DY, Kueffer LE, Juneja P, Wales TE, Engen JR, Andreotti AH
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 68 nm3

SASDRE9 – Bruton's Tyrosine Kinase - SH3-SH2-kinase domain with A384P, S386P, T387P, A388P and L390F mutations

Tyrosine-protein kinase BTK (SH3-SH2-kinase domain A384P, S386P, T387P, A388P, L390F) experimental SAS data
DAMMIF model
Sample: Tyrosine-protein kinase BTK (SH3-SH2-kinase domain A384P, S386P, T387P, A388P, L390F) monomer, 53 kDa Mus musculus protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 2% v/v glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Dec 9
Conformational heterogeneity of the BTK PHTH domain drives multiple regulatory states. Elife 12 (2024)
Lin DY, Kueffer LE, Juneja P, Wales TE, Engen JR, Andreotti AH
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 71 nm3

SASDBJ9 – Geobacillus stearothermophilus DnaB1-300

Geobacillus stearothermophilus DnaB1-300 experimental SAS data
GASBOR model
Sample: Geobacillus stearothermophilus DnaB1-300 tetramer, 138 kDa Geobacillus stearothermophilus protein
Buffer: 20 mM Tris, 300 mM NaCl and 5 mM β-ME, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 Oct 18
Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein. J Biol Chem 292(38):15744-15757 (2017)
...Lin MG, Hsiao CD
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 315 nm3

SASDBK9 – Geobacillus stearothermophilus DnaB full-length

Geobacillus stearothermophilus DnaB full-length experimental SAS data
GASBOR model
Sample: Geobacillus stearothermophilus DnaB full-length tetramer, 214 kDa Geobacillus stearothermophilus protein
Buffer: 20 mM Tris, 300 mM NaCl and 5 mM β-ME, pH: 8
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2015 Mar 12
Structural analyses of the bacterial primosomal protein DnaB reveal that it is a tetramer and forms a complex with a primosomal re-initiation protein. J Biol Chem 292(38):15744-15757 (2017)
...Lin MG, Hsiao CD
RgGuinier 5.7 nm
Dmax 20.0 nm
VolumePorod 432 nm3

SASDCT2 – Bromodomain-containing protein 2 (BRD2) tandem bromodomains

Bromodomain-containing protein 2 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 2 monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.7 nm
Dmax 21.0 nm
VolumePorod 220 nm3

SASDM47 – NAD glycohydrolase (NADase)

NAD glycohydrolase experimental SAS data
BILBOMD model
Sample: NAD glycohydrolase monomer, 47 kDa Streptococcus pyogenes M1 … protein
Buffer: ...line, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 16
Structural basis underlying the synergism of NADase and SLO during group A Streptococcus infection. Commun Biol 6(1):124 (2023)
...Lin YS, Liu CC, Senda T, Wang S
RgGuinier 3.0 nm
Dmax 103.0 nm
VolumePorod 66 nm3

SASDEU7 – Stator protein FlaG soluble domain

Conserved flagellar protein FlaG soluble domain experimental SAS data
Conserved flagellar protein FlaG soluble domain Kratky plot
Sample: Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 133 nm3

SASDCS2 – Bromodomain-containing protein 3 (BRD3) tandem bromodomains

Bromodomain-containing protein 3 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 3 monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.2 nm
Dmax 21.5 nm
VolumePorod 210 nm3

SASDM57 – NAD glycohydrolase (NADase)/Streptolysin O (SLO) complex (SAXS with additional contrast variation SANS data)

NAD glycohydrolaseStreptolysin O (T66M) experimental SAS data
BILBOMD model
Sample: NAD glycohydrolase monomer, 47 kDa Streptococcus pyogenes M1 … protein
Streptolysin O (T66M) monomer, 63 kDa Streptococcus pyogenes serotype … protein
Buffer: ...line, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 16
Structural basis underlying the synergism of NADase and SLO during group A Streptococcus infection. Commun Biol 6(1):124 (2023)
...Lin YS, Liu CC, Senda T, Wang S
RgGuinier 4.8 nm
Dmax 18.4 nm
VolumePorod 125 nm3

SASDES7 – Stator protein complex FlaG/FlaF

Conserved flagellar protein FStator protein FlaG soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 109 nm3

SASDCR2 – Bromodomain-containing protein 4 (BRD4) tandem bromodomains

Bromodomain-containing protein 4 experimental SAS data
DAMMIN model
Sample: Bromodomain-containing protein 4 monomer, 47 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 6.3 nm
Dmax 22.0 nm
VolumePorod 251 nm3

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y

Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain experimental SAS data
BILBOMD model
Sample: Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 90 nm3

SASDCU2 – Bromodomain testis-specific protein (BRDT) tandem bromodomains

Bromodomain testis-specific protein experimental SAS data
DAMMIN model
Sample: Bromodomain testis-specific protein monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 13
Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. Mol Cell (2018)
...Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC
RgGuinier 5.1 nm
Dmax 20.0 nm
VolumePorod 200 nm3

SASDET7 – Stator protein complex FlaG-V118K/FlaF

Conserved flagellar protein FStator protein FlaG-V118K soluble domain experimental SAS data
MULTIFOXS model
Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein
Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 108 nm3