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212 hits found for Chen

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDR32 – Human derived autoantibody mAb2G7 at pH 8.0

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: phosphate buffered saline, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 260 nm3

SASDF42 – Human serum albumin in complex with somapacitan

Human serum albuminSomapacitan experimental SAS data
SASREF model
Sample: Human serum albumin monomer, 66 kDa Homo sapiens protein
Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer: 100 mM MES, 140 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4
Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative. Biochemistry (2020)
...Chen J, Jensen S, Petersen J, Thygesen P
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 202 nm3

SASDR42 – Human derived autoantibody mAb5F6 at pH 8.0

Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: phosphate buffered saline, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 5.0 nm
Dmax 15.8 nm
VolumePorod 252 nm3

SASDF52 – dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE

Dosage compensation regulator experimental SAS data
dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE Rg histogram
Sample: Dosage compensation regulator monomer, 29 kDa Drosophila melanogaster protein
Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29
Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless. Nucleic Acids Res 47(8):4319-4333 (2019)
...Chen PC, Simon B, Thomae AW, Becker PB, Hennig J
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 22 nm3

SASDK52 – TAF1 tandem bromodomain

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 26
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDR52 – Human GluN1-GluN2A NMDA receptor at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2A experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 6.6 nm
Dmax 20.4 nm
VolumePorod 1180 nm3

SASDF62 – Mixture of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, and the roX2 RNA stem-loop 7 18mer-fragment, at 1.0 mg/ml

Dosage compensation regulatorroX2 stem-loop 7, 18-mer fragment experimental SAS data
Dosage compensation regulator roX2 stem-loop 7, 18-mer fragment Kratky plot
Sample: Dosage compensation regulator monomer, 29 kDa Drosophila melanogaster protein
roX2 stem-loop 7, 18-mer fragment monomer, 12 kDa synthetic construct RNA
Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29
Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless. Nucleic Acids Res 47(8):4319-4333 (2019)
...Chen PC, Simon B, Thomae AW, Becker PB, Hennig J
RgGuinier 3.1 nm
Dmax 13.3 nm
VolumePorod 25 nm3

SASDK62 – TAF1 tandem bromodomain with inhibitor AZD6738

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 44 nm3

SASDR62 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb2G7 at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2AHuman derived autoantibody mAb2G7 heavy chain, mAb2G7 VHHuman derived autoantibody mAb2G7 light chain, mAb2G7 VL experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH Human derived autoantibody mAb2G7 light chain, mAb2G7 VL Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb2G7 heavy chain, mAb2G7 VH dimer, 103 kDa protein
Human derived autoantibody mAb2G7 light chain, mAb2G7 VL dimer, 51 kDa protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 7.7 nm
Dmax 25.4 nm
VolumePorod 1260 nm3

SASDF72 – roX2 RNA stem-loop 7 18mer-fragment

roX2 stem-loop 7, 18-mer fragment experimental SAS data
DAMMIN model
Sample: roX2 stem-loop 7, 18-mer fragment monomer, 12 kDa synthetic construct RNA
Buffer: 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 29
Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless. Nucleic Acids Res 47(8):4319-4333 (2019)
...Chen PC, Simon B, Thomae AW, Becker PB, Hennig J
RgGuinier 1.8 nm
Dmax 8.5 nm
VolumePorod 14 nm3

SASDK72 – TAF1 tandem bromodomain with inhibitor BAY299

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 106 nm3

SASDR72 – Human GluN1-GluN2A NMDA receptor in complex with human derived autoantibody mAb5F6 at pH 8.0

Glutamate receptor ionotropic, NMDA 1Glutamate receptor ionotropic, NMDA 2AHuman derived autoantibody mAb5F6 heavy chain, mAb5F6 VHHuman derived autoantibody mAb5F6 light chain, mAb5F6 VL experimental SAS data
Glutamate receptor ionotropic, NMDA 1 Glutamate receptor ionotropic, NMDA 2A Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH Human derived autoantibody mAb5F6 light chain, mAb5F6 VL Kratky plot
Sample: Glutamate receptor ionotropic, NMDA 1 dimer, 193 kDa Homo sapiens protein
Glutamate receptor ionotropic, NMDA 2A dimer, 191 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 heavy chain, mAb5F6 VH dimer, 104 kDa Homo sapiens protein
Human derived autoantibody mAb5F6 light chain, mAb5F6 VL dimer, 52 kDa protein
Buffer: 150 mM NaCl, 0.1% digitonin, 5 µM Cholesteryl Hemisuccinate TRIS Salt, 0.1 mM CHAPSO, 50 µM EDTA,1 mM Gly/Glu, 20 mM HEPES, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Dec 8
Structural basis for antibody-mediated NMDA receptor clustering and endocytosis in autoimmune encephalitis. Nat Struct Mol Biol (2024)
...Chen S, Chen X, Yuan TF, Zhu S
RgGuinier 9.9 nm
Dmax 31.7 nm
VolumePorod 2500 nm3

SASDK82 – Full-length nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV)

NsP2 protein experimental SAS data
CORAL model
Sample: NsP2 protein monomer, 90 kDa Chikungunya virus protein
Buffer: 20 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, 5% glycerol, pH: 7.4
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Aug 10
Inter-domain Flexibility of Chikungunya Virus nsP2 Helicase-Protease Differentially Influences Viral RNA Replication and Infectivity. J Virol (2020)
...Chen MW, Jeng US, Merits A, Luo D
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 134 nm3

SASDR82 – Alpha cyclodextrin - 50 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDK92 – Interferon-activable protein 204 from Mus musculus (Mouse) amino-acids 215-619

Interferon-activable protein 204 experimental SAS data
CHIMERA model
Sample: Interferon-activable protein 204 monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 100 mM KCl, pH: 7.4
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Mar 14
Structural mechanism of DNA recognition by the p204 HIN domain. Nucleic Acids Res (2021)
...Chen F, Ma H, Smith P, Unterholzner L, Xiao TS, Jin T
RgGuinier 3.1 nm
Dmax 9.5 nm
VolumePorod 28 nm3

SASDR92 – Alpha cyclodextrin - 25 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDRA2 – Alpha cyclodextrin - 12.5 mg/ml aqueous solution measured to 235 inverse nm

alpha cyclodextrin experimental SAS data
alpha cyclodextrin Kratky plot
Sample: alpha cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.6 nm

SASDRB2 – Beta cyclodextrin - 18 mg/ml aqueous solution measured to 238 inverse nm

Beta Cyclodextrin experimental SAS data
Beta Cyclodextrin Kratky plot
Sample: Beta Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDRC2 – Beta cyclodextrin - 12.5 mg/ml aqueous solution measured to 238 inverse nm

Beta Cyclodextrin experimental SAS data
Beta Cyclodextrin Kratky plot
Sample: Beta Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDED2 – Polyglutamine tract-binding protein 1 (PQBP-1)

Polyglutamine-binding protein 1 experimental SAS data
Polyglutamine tract-binding protein 1 (PQBP-1) Rg histogram
Sample: Polyglutamine-binding protein 1 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 18
Solution model of the intrinsically disordered polyglutamine tract-binding protein-1. Biophys J 102(7):1608-16 (2012)
...Chen YW
RgGuinier 3.7 nm
Dmax 13.0 nm
VolumePorod 51 nm3

SASDRD2 – Gamma cyclodextrin - 50 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDEE2 – The N-terminal domain of estrogen receptor alpha

Estrogen receptor experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2017 Jun 12
A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain. Structure 27(2):229-240.e4 (2019)
...Chen Y, Agrawal P, Zheng W, Shi W, Jiang W, Yang L, Chance MR, Surewicz WK, Buck M, Yang S
RgGuinier 3.0 nm
Dmax 10.0 nm

SASDPE2 – Starch adherence system protein 6 (Sas6)

Dockerin type I repeat experimental SAS data
MULTIFOXS model
Sample: Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
...Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 97 nm3

SASDRE2 – Gamma cyclodextrin - 25 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDKF2 – Mixed-sequence DNA dimer (25 base pair DNA)

Mixed-sequence 25 base-paired DNA experimental SAS data
Mixed-sequence 25 base-paired DNA Kratky plot
Sample: Mixed-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 17 nm3

SASDRF2 – Gamma cyclodextrin - 12.5 mg/ml aqueous solution measured to 238 inverse nm

Gamma Cyclodextrin experimental SAS data
Gamma Cyclodextrin Kratky plot
Sample: Gamma Cyclodextrin monomer, 1 kDa
Buffer: water, pH: 7
Experiment: SAXS data collected at 11-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 2
Extended q‐range X‐ray Scattering Reveals High‐Resolution Structural Details of Biomacromolecules in Aqueous Solutions Chemistry – A European Journal (2023)
Chen J, Borkiewicz O, Grishaev A, Zhang F, Bera M, Ruett U, Levin I
RgGuinier 0.7 nm

SASDUF2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (P-Rex1-DH/PH-DEP1 (WT))

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
Structural and dynamic changes in P-Rex1 upon activation by PIP3 and inhibition by IP4 eLife 12 (2024)
...Chen C, Cianfrocco M, Silvio Gutkind J, Cash J, Tesmer J
RgGuinier 3.0 nm
Dmax 9.7 nm
VolumePorod 79 nm3

SASDHG2 – 12 base-paired RNA double helix (RNA12) with 30 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 30 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDKG2 – AT-sequence DNA dimer (25 base pair DNA)

AT-sequence 25 base-paired DNA experimental SAS data
AT-sequence 25 base-paired DNA Kratky plot
Sample: AT-sequence 25 base-paired DNA dimer, 16 kDa DNA
Buffer: 1.0 mM MgCl2, 10 mM Na-MOPS and 20uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
The structural plasticity of nucleic acid duplexes revealed by WAXS and MD. Sci Adv 7(17) (2021)
...Chen YL, Pollack L, Kirmizialtin S
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 16 nm3

SASDUG2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein A170K mutant (P-Rex1-DH/PH-DEP1-A170K)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (A170K) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (A170K) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 24
Structural and dynamic changes in P-Rex1 upon activation by PIP3 and inhibition by IP4 eLife 12 (2024)
...Chen C, Cianfrocco M, Silvio Gutkind J, Cash J, Tesmer J
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 76 nm3

SASDHH2 – 12 base-paired RNA double helix (RNA12) with 50 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 50 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDUH2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein L177E mutant (P-Rex1-DH/PH-DEP1-L177E)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (L177E) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (L177E) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
Structural and dynamic changes in P-Rex1 upon activation by PIP3 and inhibition by IP4 eLife 12 (2024)
...Chen C, Cianfrocco M, Silvio Gutkind J, Cash J, Tesmer J
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 88 nm3

SASDHJ2 – 12 base-paired RNA double helix (RNA12) with 100 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 100 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.5 nm

SASDUJ2 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein I409A mutant (P-Rex1-DH/PH-DEP1-I409A)

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (I409A) experimental SAS data
EOM/RANCH model
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (I409A) monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES pH 7, 300 mM NaCl, 2% glycerol, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 8
Structural and dynamic changes in P-Rex1 upon activation by PIP3 and inhibition by IP4 eLife 12 (2024)
...Chen C, Cianfrocco M, Silvio Gutkind J, Cash J, Tesmer J
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 79 nm3

SASDHK2 – 12 base-paired RNA double helix (RNA12) with 200 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 200 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHL2 – 12 base-paired RNA double helix (RNA12) with 500 mM KCl - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 500 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDUL2 – mRNA capping enzyme small subunit

Virus termination factor small subunit experimental SAS data
DAMFILT model
Sample: Virus termination factor small subunit monomer, 33 kDa Monkeypox virus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 Jan 4
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 13(1):2369193 (2024)
Chen A, Fang N, Zhang Z, Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J
RgGuinier 2.4 nm
Dmax 6.8 nm
VolumePorod 51 nm3

SASDHM2 – 12 base-paired RNA double helix (RNA12) with 0.25 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 0.25 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.4 nm

SASDUM2 – mRNA capping enzyme MTase

Virus termination factor small subunitmRNA-capping enzyme catalytic subunit experimental SAS data
DAMFILT model
Sample: Virus termination factor small subunit monomer, 33 kDa Monkeypox virus (strain … protein
mRNA-capping enzyme catalytic subunit monomer, 35 kDa Monkeypox virus (strain … protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2024 Jan 4
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 13(1):2369193 (2024)
Chen A, Fang N, Zhang Z, Wen Y, Shen Y, Zhang Y, Zhang L, Zhao G, Ding J, Li J
RgGuinier 2.9 nm
Dmax 10.1 nm
VolumePorod 106 nm3

SASDHN2 – 12 base-paired RNA double helix (RNA12) with 1 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 1.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.6 nm

SASDHP2 – 12 base-paired RNA double helix (RNA12) with 5 mM MgCl2 - SWAXS

12 base-paired RNA double helix experimental SAS data
12 base-paired RNA double helix Kratky plot
Sample: 12 base-paired RNA double helix monomer, 8 kDa RNA
Buffer: 5.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDHQ2 – 25 base-paired RNA double helix (RNA25) with 100 mM NaCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 100 mM NaCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.1 nm

SASDHR2 – 25 base-paired RNA double helix (RNA25) with 10 mM MgCl2 - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 10.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.6 nm

SASDHS2 – 25 base-paired RNA double helix (RNA25) with 400 mM KCl - WAXS

25 base-paired RNA double helix experimental SAS data
25 base-paired RNA double helix Kratky plot
Sample: 25 base-paired RNA double helix monomer, 16 kDa RNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 13
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.3 nm

SASDHT2 – 25 base-paired DNA double helix (DNA25) with 400 mM KCl - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 400 mM KCl, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKT2 – Wild type double Element for Nuclear Expression (dENE)

Wild type dENE experimental SAS data
Wild type dENE Kratky plot
Sample: Wild type dENE monomer, 23 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.8 nm
VolumePorod 31 nm3

SASDHU2 – 25 base-paired DNA double helix (DNA25) with 0.5 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 0.5 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 2.0 nm

SASDKU2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)20

Wild type dENE + Poly(A)20 experimental SAS data
Wild type dENE + Poly(A)20 Kratky plot
Sample: Wild type dENE + Poly(A)20 monomer, 30 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.8 nm
Dmax 10.7 nm

SASDHV2 – 25 base-paired DNA double helix (DNA25) with 2 mM MgCl2 - SWAXS

25 base-paired DNA double helix experimental SAS data
25 base-paired DNA double helix Kratky plot
Sample: 25 base-paired DNA double helix monomer, 15 kDa DNA
Buffer: 2.0 mM MgCl2, 20 mM KMOPS, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 16
Salt Dependence of A-Form RNA Duplexes: Structures and Implications. J Phys Chem B 123(46):9773-9785 (2019)
Chen YL, Pollack L
RgGuinier 1.9 nm

SASDKV2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)25

Wild type dENE + Poly(A)25 experimental SAS data
Wild type dENE + Poly(A)25 Kratky plot
Sample: Wild type dENE + Poly(A)25 monomer, 31 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.4 nm

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30 experimental SAS data
Wild type dENE + Poly(A)30 Kratky plot
Sample: Wild type dENE + Poly(A)30 monomer, 33 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.6 nm

SASDTW2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme wildtype

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H)Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform experimental SAS data
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Kratky plot
Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H) monomer, 70 kDa Homo sapiens protein
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20
B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations. Proc Natl Acad Sci U S A 121(1):e2310727120 (2024)
...Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y
RgGuinier 4.0 nm
Dmax 12.0 nm
VolumePorod 266 nm3

SASDKX2 – double Element for Nuclear Expression (dENE) Mutant A

A dENE experimental SAS data
A dENE Kratky plot
Sample: A dENE monomer, 27 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 10.9 nm

SASDTX2 – Protein phosphatase 2A (PP2A)-B56δ holoenzyme E198K mutant

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoformIsoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformIsoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) experimental SAS data
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) Kratky plot
Sample: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform monomer, 65 kDa Homo sapiens protein
Isoform 1 of Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform monomer, 36 kDa Homo sapiens protein
Isoform Delta-1 of Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (R160H, E198K) monomer, 70 kDa Homo sapiens protein
Buffer: 25 mM Tris, 150 mM NaCl, 50 µM MnCl2, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Nov 20
B56δ long-disordered arms form a dynamic PP2A regulation interface coupled with global allostery and Jordan's syndrome mutations. Proc Natl Acad Sci U S A 121(1):e2310727120 (2024)
...Chen YC, Xu Z, Wei H, Sundaresan R, Cui Q, Wadzinski BE, Swingle MR, Musiyenko A, Chung WK, Honkanen RE, Suzuki A, Huang X, Strack S, Xing Y
RgGuinier 4.2 nm
Dmax 14.0 nm
VolumePorod 305 nm3

SASDKY2 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)20

A dENE + Poly(A)20 experimental SAS data
A dENE + Poly(A)20 Kratky plot
Sample: A dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.5 nm

SASDK23 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)30

A dENE + Poly(A)30 experimental SAS data
A dENE + Poly(A)30 Kratky plot
Sample: A dENE + Poly(A)30 monomer, 37 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 13.3 nm

SASDJ33 – Receptor-type tyrosine-protein phosphatase epsilon bound to phospho proto-oncogene tyrosine-protein kinase (rPTPε/phospho-Src complex)

Receptor-type tyrosine-protein phosphatase epsilonProto-oncogene tyrosine-protein kinase Src, T357M mutant experimental SAS data
CORAL model
Sample: Receptor-type tyrosine-protein phosphatase epsilon monomer, 34 kDa Homo sapiens protein
Proto-oncogene tyrosine-protein kinase Src, T357M mutant monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris , 50 mM NaCl, 5 mM DTT, pH: 8
Experiment: SAXS data collected at 23A1, Taiwan Photon Source, NSRRC on 2017 May 26
An integrative approach unveils a distal encounter site for rPTPε and phospho-Src complex formation Structure (2023)
...Chen G, Yeh Y, Yang H, Ho M
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 60 nm3

SASDK33 – double Element for Nuclear Expression (dENE) Mutant B

B dENE experimental SAS data
B dENE Kratky plot
Sample: B dENE monomer, 24 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 2.9 nm
Dmax 10.8 nm

SASDK43 – double Element for Nuclear Expression (dENE) Mutant B + Poly(A)30

B dENE + Poly(A)30 experimental SAS data
B dENE + Poly(A)30 Kratky plot
Sample: B dENE + Poly(A)30 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDK63 – double Element for Nuclear Expression (dENE) Mutant C

C dENE experimental SAS data
C dENE Kratky plot
Sample: C dENE monomer, 29 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.4 nm
Dmax 12.4 nm

SASDK73 – double Element for Nuclear Expression (dENE) Crystal Structure

Xtal dENE experimental SAS data
Xtal dENE Kratky plot
Sample: Xtal dENE monomer, 28 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.1 nm
Dmax 11.0 nm

SASDK83 – double Element for Nuclear Expression (dENE) Mutant C + Poly(A)30

C dENE + Poly(A)30 experimental SAS data
C dENE + Poly(A)30 Kratky plot
Sample: C dENE + Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.6 nm
Dmax 14.8 nm

SASDKA3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)25

Xtal dENE + Poly(A)25 experimental SAS data
Xtal dENE + Poly(A)25 Kratky plot
Sample: Xtal dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKB3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)30

Xtal dENE + Poly(A)30 experimental SAS data
Xtal dENE + Poly(A)30 Kratky plot
Sample: Xtal dENE + Poly(A)30 monomer, 38 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 13.2 nm

SASDKC3 – double Element for Nuclear Expression (dENE) Mutant A + Poly(A)25

A dENE + Poly(A)25 experimental SAS data
A dENE + Poly(A)25 Kratky plot
Sample: A dENE + Poly(A)25 monomer, 36 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.3 nm
Dmax 12.4 nm

SASDKD3 – double Element for Nuclear Expression (dENE) Mutant B + Hairpin - Poly(A)30

B dENE + Hairpin - Poly(A)30 experimental SAS data
B dENE + Hairpin - Poly(A)30 Kratky plot
Sample: B dENE + Hairpin - Poly(A)30 monomer, 39 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.5 nm
Dmax 14.0 nm

SASDKE3 – double Element for Nuclear Expression (dENE) Crystal Structure + Poly(A)20

Xtal dENE + Poly(A)20 experimental SAS data
Xtal dENE + Poly(A)20 Kratky plot
Sample: Xtal dENE + Poly(A)20 monomer, 34 kDa RNA
Buffer: 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2019 Dec 5
Structural analyses of an RNA stability element interacting with poly(A) Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021)
...Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J
RgGuinier 3.2 nm
Dmax 11.8 nm

SASDBL3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 10
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
...Chen SY, Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 53 nm3

SASDBN3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 7
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
...Chen SY, Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 55 nm3

SASDPT3 – Histone-lysine N-methyltransferase multi protein complex: MLL1-WDR5-RBBP5-ASH2L

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Retinoblastoma-binding protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2 experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. iScience 25(9):104948 (2022)
...Chen Y
RgGuinier 5.2 nm
Dmax 18.5 nm
VolumePorod 386 nm3

SASDMU3 – Overall conformation and interdomain arrangement of Vibrio collagenase VhaC in solution.

Vibrio collagenase VhaC experimental SAS data
CORAL model
Sample: Vibrio collagenase VhaC monomer, 90 kDa Vibrio harveyi protein
Buffer: 10 mM Tris-HCl, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Jan 1
Structure of Vibrio collagenase VhaC provides insight into the mechanism of bacterial collagenolysis. Nat Commun 13(1):566 (2022)
...Chen Y, Chen XL, Zhang YZ
RgGuinier 4.3 nm
Dmax 17.6 nm
VolumePorod 148 nm3

SASDPU3 – Histone-lysine N-methyltransferase multi protein complex bound to protein dpy-30 homologue: MLL1-WDR5-RBBP5-ASH2L-DPY30

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Retinoblastoma-binding protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Protein dpy-30 homolog experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Retinoblastoma-binding protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Protein dpy-30 homolog Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 37 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Protein dpy-30 homolog dimer, 23 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 150 mM NaCl, 1 mM TECP, pH: 7.4
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. iScience 25(9):104948 (2022)
...Chen Y
RgGuinier 5.4 nm
Dmax 17.5 nm
VolumePorod 411 nm3

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1 experimental SAS data
ITASSER model
Sample: Macrophage mannose receptor 1 dimer, 315 kDa Mouse myeloma cell … protein
Buffer: 50mM Hepes, 100mM NaCl, 1mM DTT, pH: 7
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2016 Apr 15
Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses. Sci Transl Med 12(530) (2020)
...Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M, Southall N, Ock CY,...
RgGuinier 7.9 nm
Dmax 30.1 nm
VolumePorod 584 nm3

SASDGD4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-381)

Retinoblastoma-binding protein 5Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2 experimental SAS data
Retinoblastoma-binding protein 5 Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Kratky plot
Sample: Retinoblastoma-binding protein 5 monomer, 42 kDa Homo sapiens protein
Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22
The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. Nucleic Acids Res (2019)
...Chen Y
RgGuinier 5.7 nm
Dmax 18.6 nm
VolumePorod 360 nm3

SASDGE4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-480)

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Retinoblastoma-binding protein 5 experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 53 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22
The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. Nucleic Acids Res (2019)
...Chen Y
RgGuinier 5.0 nm
Dmax 15.3 nm
VolumePorod 256 nm3

SASDGF4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-480)L399A/L400A/I457A/L459A

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Retinoblastoma-binding protein 5 experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 53 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22
The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. Nucleic Acids Res (2019)
...Chen Y
RgGuinier 5.3 nm
Dmax 17.2 nm
VolumePorod 313 nm3

SASDGG4 – Mixed lineage leukemia protein-1 complex, MLL1-WDR5-ASH2L-RBBP5(2-538)

Histone-lysine N-methyltransferase 2AWD repeat-containing protein 5Set1/Ash2 histone methyltransferase complex subunit ASH2Retinoblastoma-binding protein 5 experimental SAS data
Histone-lysine N-methyltransferase 2A WD repeat-containing protein 5 Set1/Ash2 histone methyltransferase complex subunit ASH2 Retinoblastoma-binding protein 5 Kratky plot
Sample: Histone-lysine N-methyltransferase 2A monomer, 25 kDa Homo sapiens protein
WD repeat-containing protein 5 monomer, 34 kDa Homo sapiens protein
Set1/Ash2 histone methyltransferase complex subunit ASH2 monomer, 60 kDa Homo sapiens protein
Retinoblastoma-binding protein 5 monomer, 59 kDa Homo sapiens protein
Buffer: 300 mM NaCl, 25mM Tris-HCl, 4% glycerol, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2019 Jun 22
The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. Nucleic Acids Res (2019)
...Chen Y
RgGuinier 5.0 nm
Dmax 15.5 nm
VolumePorod 282 nm3

SASDDX4 – Wild-type Protein sex-lethal

Protein sex-lethal experimental SAS data
Protein sex-lethal Kratky plot
Sample: Protein sex-lethal monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 16
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 26 nm3

SASDDY4 – Protein sex-lethal mutant with 10GS-linker

Protein sex-lethal mutant experimental SAS data
Protein sex-lethal mutant with 10GS-linker Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
Buffer: 10 mM KP, 50 mM NaCl, 10 mM DTT, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 2
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 27 nm3

SASDDZ4 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU

Protein sex-lethal mutantRNA decaneucleotide U8GU experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide U8GU Rg histogram
Sample: Protein sex-lethal mutant monomer, 20 kDa Drosophila melanogaster protein
RNA decaneucleotide U8GU monomer, 3 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 3
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.2 nm
Dmax 7.8 nm
VolumePorod 34 nm3

SASDD25 – 1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8

Protein sex-lethal mutantRNA decaneucleotide UGU8 experimental SAS data
1:1 Mixture between Protein sex-lethal mutant (Sxl10GS) and RNA decaneucleotide UGU8 Rg histogram
Sample: Protein sex-lethal mutant dimer, 41 kDa Drosophila melanogaster protein
RNA decaneucleotide UGU8 dimer, 6 kDa synthetic construct RNA
Buffer: 50% dilution of protein buffer {10 mM KP, 50 mM NaCl, 10 mM DTT pH 6} with {milliQ-water pH 7} suspended RNA, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2017 Apr 12
A General Small-Angle X-ray Scattering-Based Screening Protocol Validated for Protein-RNA Interactions. ACS Comb Sci 20(4):197-202 (2018)
Chen PC, Masiewicz P, Rybin V, Svergun D, Hennig J
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 41 nm3

SASDA85 – CHD4 (PP-CC-AH-D)

Human Chromatin Remodeler CHD4 (363-1353) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (363-1353) monomer, 117 kDa Homo sapiens protein
Buffer: 50 mM HEPES 5% Glycerol 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.9 nm
Dmax 17.4 nm

SASDA95 – CHD4 (CC-AH-D)

Human Chromatin Remodeler CHD4 (494-1353) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (494-1353) monomer, 101 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.0 nm
Dmax 14.5 nm

SASDAA5 – CHD4 (AH)

Human Chromatin Remodeler CHD4 (685-1233) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (685-1233) monomer, 63 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 4.0 nm
Dmax 14.5 nm

SASDAB5 – CHD4 (PP-CC)

Human Chromatin Remodeler CHD4 (363-682) experimental SAS data
DAMMIF model
Sample: Human Chromatin Remodeler CHD4 (363-682) monomer, 38 kDa Homo sapiens protein
Buffer: 50 mM HEPES 50 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 13
The PHD and chromo domains regulate the ATPase activity of the human chromatin remodeler CHD4. J Mol Biol 422(1):3-17 (2012)
...Chen Y, Burgess-Brown NA, Kouzarides T, Wiechens N, Owen-Hughes T, Svergun DI, Gileadi O, Laue ED
RgGuinier 3.0 nm
Dmax 10.5 nm

SASDHB5 – RelA Homology Domain of p50/RelA heterodimer

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321 experimental SAS data
MULTIFOXS model
Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 3.8 nm
Dmax 13.0 nm
VolumePorod 110 nm3

SASDHC5 – Nuclear factor kB p50/RelA heterodimer

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350 experimental SAS data
BILBOMD model
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.6 nm
Dmax 15.3 nm
VolumePorod 183 nm3

SASDHD5 – Transactivation domain of RelA

Transcription factor p65 340-549 experimental SAS data
MULTIFOXS model
Sample: Transcription factor p65 340-549 monomer, 23 kDa Mus musculus protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 59 nm3

SASDHE5 – Rel Homology Domain of p50/RelA bound to IFN kB site DNA

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321IFN kB DNA experimental SAS data
NF-kappa-B p105 subunit 39-350 Transcription factor p65 19-321 IFN kB DNA Kratky plot
Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
IFN kB DNA monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.3 nm
Dmax 13.0 nm
VolumePorod 237 nm3

SASDQE5 – Nucleoside triphosphate pyrophosphohydrolase (1–185)

Nucleoside triphosphate pyrophosphohydrolase experimental SAS data
DAMMIF model
Sample: Nucleoside triphosphate pyrophosphohydrolase dimer, 41 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2020 Dec 11
Structural analysis of the housecleaning nucleoside triphosphate pyrophosphohydrolase MazG from Mycobacterium tuberculosis Frontiers in Microbiology 14 (2023)
...Chen A, Zhang Z, Wang S, Lv L, Zhao G, Li J
RgGuinier 2.7 nm
Dmax 8.4 nm
VolumePorod 64 nm3

SASDHF5 – Rel Homology Domain of p50/RelA bound to Urokinase kB site DNA

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321Urokinase kB experimental SAS data
NF-kappa-B p105 subunit 39-350 Transcription factor p65 19-321 Urokinase kB Kratky plot
Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
Urokinase kB monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 244 nm3

SASDHG5 – Nuclear factor kB p50/RelA bound to IFN kB site DNA

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350IFN kB DNA experimental SAS data
Transcription factor p65 19-549 NF-kappa-B p105 subunit 39-350 IFN kB DNA Kratky plot
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
IFN kB DNA monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 5.1 nm
Dmax 18.9 nm
VolumePorod 314 nm3

SASDKG5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDHH5 – Nuclear factor kB p50/RelA bound to Urokinase kB site DNA

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350Urokinase kB experimental SAS data
Transcription factor p65 19-549 NF-kappa-B p105 subunit 39-350 Urokinase kB Kratky plot
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Urokinase kB monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
...Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.7 nm
Dmax 17.5 nm
VolumePorod 201 nm3

SASDKH5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKJ5 – 25 Based-Paired DNA Duplex

25 base-paired DNA Duplex experimental SAS data
25 base-paired DNA Duplex Kratky plot
Sample: 25 base-paired DNA Duplex monomer, 15 kDa DNA
Buffer: 100 mM NaCl, 10 mM sodium 3-(N-morpholino)propanesulfonic acid (Na-MOPS) and 20 uM EDTA, pH: 7
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.3 nm
Dmax 8.4 nm

SASDKK5 – RNA Short Tetraloop Hairpin Duplex

RNA Short Tetraloop Hairpin Duplex experimental SAS data
RNA Short Tetraloop Hairpin Duplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Duplex monomer, 13 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Jun 7
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 8.9 nm

SASDKL5 – RNA Short Tetraloop Hairpin Triplex

RNA Short Tetraloop Hairpin Triplex experimental SAS data
RNA Short Tetraloop Hairpin Triplex Kratky plot
Sample: RNA Short Tetraloop Hairpin Triplex monomer, 17 kDa RNA
Buffer: 200mM NaCl, 1.0mM MgCl2, 20mM Na-MES, 50µM EDTA, pH: 5.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2019 Sep 20
Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids
Yen-Lin Chen
RgGuinier 2.0 nm
Dmax 6.3 nm