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72 hits found for Yang

SASDK52 – TAF1 tandem bromodomain

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 26
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDK62 – TAF1 tandem bromodomain with inhibitor AZD6738

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 44 nm3

SASDK72 – TAF1 tandem bromodomain with inhibitor BAY299

Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
...Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 106 nm3

SASDEE2 – The N-terminal domain of estrogen receptor alpha

Estrogen receptor experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor monomer, 20 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP, pH: 7.4
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2017 Jun 12
A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain. Structure 27(2):229-240.e4 (2019)
...Yang L, Chance MR, Surewicz WK, Buck M, Yang S
RgGuinier 3.0 nm
Dmax 10.0 nm

SASDRG2 – Ligand free Xenosiderophore Utilization System B (XusB)

DUF4374 domain-containing protein experimental SAS data
Sample: DUF4374 domain-containing protein monomer, 46 kDa Bacteroides thetaiotaomicron (strain … protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 May 9
Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection. Cell Host Microbe 31(10):1639-1654.e10 (2023)
...Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 74 nm3

SASDRH2 – Ligand bound Xenosiderophore Utilization System B (XusB)

DUF4374 domain-containing protein experimental SAS data
Sample: DUF4374 domain-containing protein monomer, 46 kDa Bacteroides thetaiotaomicron (strain … protein
Buffer: 20 mM Tris 150 mM NaCl, pH: 8
Experiment: SAXS data collected at 12-ID-B, Advanced Photon Source (APS), Argonne National Laboratory on 2021 May 9
Iron acquisition by a commensal bacterium modifies host nutritional immunity during Salmonella infection. Cell Host Microbe 31(10):1639-1654.e10 (2023)
...Yang ZJ, Skaar EP, Byndloss MX, Chazin WJ, Zhu W
RgGuinier 2.4 nm
Dmax 7.4 nm
VolumePorod 77 nm3

SASDBL2 – MBP-PICK1 fusion

Maltose Binding Protein fused to Protein Interacting with C kinase 1 experimental SAS data
CRYSOL model
Sample: Maltose Binding Protein fused to Protein Interacting with C kinase 1 dimer, 174 kDa Homo sapiens protein
Buffer: 50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 13
PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner. Mol Biol Cell 26(7):1308-22 (2015)
...Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R
RgGuinier 8.4 nm
Dmax 27.6 nm
VolumePorod 483 nm3

SASDLP2 – Outer membrane associated protein, FopA dimer in Tris-HCl, NaCl and n-Dodecyl beta-D-maltoside

Francisella tularensis outer membrane protein A experimental SAS data
Sample: Francisella tularensis outer membrane protein A dimer, 80 kDa Francisella tularensis subsp. … protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.05% B-DDM, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 20
Structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis. PLoS One 17(8):e0267370 (2022)
...Yang JH, Goode MR, Ketawala G, Craciunescu FM, Zook JD, Sonowal M, Williams D, Grant TD, Fromme R, Hansen DT, Fromme P
RgGuinier 4.4 nm
Dmax 16.0 nm
VolumePorod 330 nm3

SASDJ33 – Receptor-type tyrosine-protein phosphatase epsilon bound to phospho proto-oncogene tyrosine-protein kinase (rPTPε/phospho-Src complex)

Receptor-type tyrosine-protein phosphatase epsilonProto-oncogene tyrosine-protein kinase Src, T357M mutant experimental SAS data
CORAL model
Sample: Receptor-type tyrosine-protein phosphatase epsilon monomer, 34 kDa Homo sapiens protein
Proto-oncogene tyrosine-protein kinase Src, T357M mutant monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris , 50 mM NaCl, 5 mM DTT, pH: 8
Experiment: SAXS data collected at 23A1, Taiwan Photon Source, NSRRC on 2017 May 26
An integrative approach unveils a distal encounter site for rPTPε and phospho-Src complex formation Structure (2023)
...Yang C, Tewary S, Peng W, Chen G, Yeh Y, Yang H, Ho M
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 60 nm3

SASDP93 – K48-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 2.6 nm
Dmax 95.0 nm
VolumePorod 44 nm3

SASDPA3 – K63-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 44 nm3

SASDPB3 – M1-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 46 nm3

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 115 nm3

SASDBL3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 10
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
...Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 53 nm3

SASDBN3 – Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens

cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant experimental SAS data
cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant Kratky plot
Sample: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Y259D mutant monomer, 46 kDa Homo sapiens protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 7
In vivo epidermal migration requires focal adhesion targeting of ACF7. Nat Commun 7:11692 (2016)
...Yang F, Liang H, Wu X
RgGuinier 3.4 nm
Dmax 13.5 nm
VolumePorod 55 nm3

SASDJY3 – Immunoglobulin HUI-018 Fab in complex with human insulin

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
...Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 86 nm3

SASDJZ3 – Immunoglobulin HUI-018 Fab in complex with human insulin at high concentration

Insulin (Insulin B chain and Insulin A chain)HUI-018 Fab experimental SAS data
OTHER model
Sample: Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Homo sapiens protein
HUI-018 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at I911-4, MAX IV on 2013 Mar 7
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
...Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 3.7 nm
Dmax 13.4 nm
VolumePorod 105 nm3

SASDJ24 – Immunoglobulin HUI-018 Fab and OXI-005 Fab in a ternary complex with porcine insulin

HUI-018 FabInsulin (Insulin B chain and Insulin A chain)OXI-005 Fab experimental SAS data
OTHER model
Sample: HUI-018 Fab monomer, 47 kDa Mus musculus protein
Insulin (Insulin B chain and Insulin A chain) monomer, 6 kDa Sus scrofa protein
OXI-005 Fab monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 140 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2019 Mar 28
Insulin Binding to the Analytical Antibody Sandwich Pair OXI ‐005 and HUI ‐018 – Epitope Mapping and Binding Properties Protein Science (2020)
...Yang Z, Cao Z, Wiberg C, Jeppesen C, Poulsen F
RgGuinier 4.5 nm
Dmax 14.2 nm
VolumePorod 138 nm3

SASDHS4 – Protein ninH from Bacteriophage lambda (N-terminal histidine tagged) T15A mutated

Protein ninH experimental SAS data
MODELLER model
Sample: Protein ninH dimer, 20 kDa Escherichia phage lambda protein
Buffer: 150 mM NaCl, 50 mM phosphate buffer (pH 7.4), 1 mM EDTA, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss 2.0 with MetalJet, Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University on 2019 Jul 1
A bacteriophage mimic of the bacterial nucleoid-associated protein Fis. Biochem J (2020)
...Yang AWH, Jolma A, Paterson JR, Świątek S, Plewka J, Curtis FA, Bowers LY, Pålsson LO, Hughes TR, Taube M, Kozak M, Heddle JG, Sharples GJ
RgGuinier 2.1 nm
Dmax 8.3 nm
VolumePorod 33 nm3

SASDT35 – N-cadherin extracellular domains EC1-EC5

Cadherin-2 experimental SAS data
Cadherin-2 Kratky plot
Sample: Cadherin-2 dimer, 123 kDa Mus musculus protein
Buffer: 10 mM HEPES, 150 mM NaCl, 3 mM CaCl2, 0.02% NaN3, pH: 8
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 1
Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Cell 187(5):1296-1311.e26 (2024)
...Yang TJ, von Bülow S, Sehrawat V, Zerrouki N, Tuffery M, Gecht M, Grothaus IL, Colombi Ciacchi L, Wang YS, Hsu MF, Khoo KH, Hummer G, Hsu SD, Hanus C, Sikora M
RgGuinier 8.8 nm
Dmax 40.0 nm
VolumePorod 496 nm3

SASDT45 – N-cadherin extracellular domains EC4-EC5

Cadherin-2 experimental SAS data
Cadherin-2 Kratky plot
Sample: Cadherin-2 monomer, 24 kDa Mus musculus protein
Buffer: 10 mM HEPES, 150 mM NaCl, 3 mM CaCl2, 0.02% NaN3, pH: 8
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2023 May 1
Rapid simulation of glycoprotein structures by grafting and steric exclusion of glycan conformer libraries. Cell 187(5):1296-1311.e26 (2024)
...Yang TJ, von Bülow S, Sehrawat V, Zerrouki N, Tuffery M, Gecht M, Grothaus IL, Colombi Ciacchi L, Wang YS, Hsu MF, Khoo KH, Hummer G, Hsu SD, Hanus C, Sikora M
RgGuinier 3.4 nm
Dmax 12.6 nm
VolumePorod 63 nm3

SASDQF5 – Zinc finger protein 410 (ZNF410 full length)

Zinc finger protein 410 experimental SAS data
BILBOMD model
Sample: Zinc finger protein 410 monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 108 nm3

SASDQG5 – DNA (Zinc finger protein 410 recognition sequence)

DNA (Zinc finger protein 410 recognition sequence) experimental SAS data
DNA (Zinc finger protein 410 recognition sequence) Kratky plot
Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 16 nm3

SASDQH5 – Zinc finger protein 410 (ZNF410 full length) bound to DNA

Zinc finger protein 410DNA (Zinc finger protein 410 recognition sequence) experimental SAS data
BILBOMD model
Sample: Zinc finger protein 410 monomer, 52 kDa Homo sapiens protein
DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 4.4 nm
Dmax 14.3 nm
VolumePorod 76 nm3

SASDQJ5 – Zinc finger protein 410 (ZNF410): N-terminal region with 1-5 zinc fingers

Zinc finger protein 410 experimental SAS data
BILBOMD model
Sample: Zinc finger protein 410 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 78 nm3

SASDQK5 – Zinc finger protein 410 (ZNF410): N-terminal region with 1-5 zinc fingers bound to DNA

DNA (Zinc finger protein 410 recognition sequence)Zinc finger protein 410 experimental SAS data
BILBOMD model
Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA
Zinc finger protein 410 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2021 Apr 25
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 3.1 nm
Dmax 14.1 nm
VolumePorod 63 nm3

SASDQL5 – Zinc finger protein 410 (ZNF410): C-terminal region with 1-5 zinc fingers

Zinc finger protein 410 experimental SAS data
BILBOMD model
Sample: Zinc finger protein 410 monomer, 29 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 3.0 nm
Dmax 9.6 nm
VolumePorod 58 nm3

SASDFM5 – Mutant 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, H110A tetramer, at pH 8.5

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase experimental SAS data
CORAL model
Sample: 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase tetramer, 159 kDa Pseudomonas fluorescens protein
Buffer: 50 mM Tris, 5 mM DTT, pH: 8.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jul 15
Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity. J Biol Chem 294(30):11609-11621 (2019)
Yang Y, Davis I, Matsui T, Rubalcava I, Liu A
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 238 nm3

SASDQM5 – Zinc finger protein 410 (ZNF410): C-terminal region with 1-5 zinc fingers bound to DNA

DNA (Zinc finger protein 410 recognition sequence)Zinc finger protein 410 experimental SAS data
BILBOMD model
Sample: DNA (Zinc finger protein 410 recognition sequence) monomer, 11 kDa DNA
Zinc finger protein 410 monomer, 29 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 0.1% v/v β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2020 Sep 30
Allosteric autoregulation of DNA binding via a DNA-mimicking protein domain: a biophysical study of ZNF410-DNA interaction using small angle X-ray scattering. Nucleic Acids Res (2023)
...Yang J, Cao Y, He C, Lan F, Lan X, Blobel GA, Blumenthal RM, Zhang X, Cheng X
RgGuinier 3.1 nm
Dmax 11.9 nm
VolumePorod 52 nm3

SASDFN5 – Wild type 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, ACMSD tetramer, at pH 7.0

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase experimental SAS data
CORAL model
Sample: 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase tetramer, 159 kDa Pseudomonas fluorescens protein
Buffer: 25 mM HEPES, 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2018 Jan 10
Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity. J Biol Chem 294(30):11609-11621 (2019)
Yang Y, Davis I, Matsui T, Rubalcava I, Liu A
RgGuinier 4.7 nm
Dmax 17.5 nm
VolumePorod 195 nm3

SASDTN5 – The D2 domain of E3 ubiquitin-protein ligase DTX3L (I101-Q200)

E3 ubiquitin-protein ligase DTX3L experimental SAS data
E3 ubiquitin-protein ligase DTX3L Kratky plot
Sample: E3 ubiquitin-protein ligase DTX3L tetramer, 46 kDa Homo sapiens protein
Buffer: 30 mM HEPES, 350 mM NaCl, 5% (v/v) glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Dec 6
Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor. bioRxiv (2023)
...Yang C, Alanen HI, Eki R, Abbas TA, Maksimainen MM, Glumoff T, Duman R, Wagner A, Paschal BM, Lehtiö L
RgGuinier 2.9 nm
Dmax 112.0 nm
VolumePorod 91 nm3

SASDCL6 – Lys63-linked dimer ubiquitin

Polyubiquitin-C experimental SAS data
Polyubiquitin-C Kratky plot
Sample: Polyubiquitin-C dimer, 17 kDa Homo sapiens protein
Buffer: 100mM NaCl, 10mM sodium acetate, pH: 6
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Mar 24
Lys63-linked ubiquitin chain adopts multiple conformational states for specific target recognition. Elife 4 (2015)
...Yang J, Zhu WK, Guo DC, Zhang WP, Liu ML, Tang C
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 24 nm3

SASDMQ6 – The N-terminus of E3 ubiquitin-protein ligase DTX3L (M1-Q200)

E3 ubiquitin-protein ligase DTX3L experimental SAS data
E3 ubiquitin-protein ligase DTX3L Kratky plot
Sample: E3 ubiquitin-protein ligase DTX3L pentamer, 114 kDa Homo sapiens protein
Buffer: 30 mM HEPES, 350 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 6
Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimerization and multimeric assembly. Biochem J (2022)
...Yang CS, Alanen HI, Liu F, Paschal BM, Lehtiö L
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 200 nm3

SASDES6 – The 11S subunit of the Plasmodium falciparum proteasome, PA28

Proteasome activator PA28 experimental SAS data
CORAL model
Sample: Proteasome activator PA28 heptamer, 232 kDa Plasmodium falciparum protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Dec 2
The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis. Nat Microbiol 4(11):1990-2000 (2019)
...Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L
RgGuinier 4.3 nm
Dmax 12.9 nm
VolumePorod 484 nm3

SASDMU7 – Apt31 - ssDNA aptamer specific to the receptor-binding domain of SARS-CoV-2

ssDNA aptamer Apt31 specific to the receptor-binding domain of SARS-CoV-2 experimental SAS data
GROMACS model
Sample: ssDNA aptamer Apt31 specific to the receptor-binding domain of SARS-CoV-2 monomer, 10 kDa Artificially synthesized DNA
Buffer: Tris-HCl, pH: 7.4
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2020 Dec 18
Structure and Interaction Based Design of Anti‐SARS‐CoV‐2 Aptamers Chemistry – A European Journal (2022)
...Yang C, Moskovsky A, Fedorov D, Tomilin F, Tan W, Alexeev Y, Berezovski M, Kichkailo A
RgGuinier 1.9 nm
Dmax 6.5 nm
VolumePorod 12 nm3

SASDNF8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer

Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.4 nm
Dmax 18.4 nm
VolumePorod 264 nm3

SASDNG8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer treated with PNGase F enzyme

Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.3 nm
Dmax 18.0 nm

SASDNH8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer

Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.2 nm
Dmax 16.5 nm

SASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme

Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Yang T, Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.1 nm
Dmax 15.9 nm
VolumePorod 231 nm3

SASDDU8 – Multidomain architecture of the estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains

Estrogen receptorERE1ERE2EstradiolhERa peptide1hERa peptide2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Estrogen receptor dimer, 85 kDa protein
ERE1 monomer, 6 kDa Homo sapiens DNA
ERE2 monomer, 6 kDa Homo sapiens DNA
Estradiol dimer, 0 kDa
hERa peptide1 monomer, 2 kDa protein
hERa peptide2 monomer, 2 kDa protein
Buffer: 10 mM CHES (pH9.5), 125 mM NaCl, 5mM KCl, 4 mM MgCl2, 50 mM arginine, 50 mM glutamate, 5 mM TCEP, 5% glycerol, 10 µm Zn acetate, 10 µM estradiol, pH: 9.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Aug 10
Multidomain architecture of estrogen receptor reveals interfacial cross-talk between its DNA-binding and ligand-binding domains. Nat Commun 9(1):3520 (2018)
...Yang S
RgGuinier 3.8 nm
Dmax 11.5 nm

SASDEB9 – Flagellar brake protein YcgR from Escherichia coli

Flagellar brake protein YcgR experimental SAS data
SASREF model
Sample: Flagellar brake protein YcgR monomer, 29 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150mM NaCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jan 4
Structural insights into the mechanism of c-di-GMP-bound YcgR regulating flagellar motility in Escherichia coli. J Biol Chem 295(3):808-821 (2020)
...Yang WS, Hong Y, Zhang Y, Wang DC, Li DF
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 44 nm3

SASDEC9 – Flagellar brake protein YcgR in complex with c-di-GMP from Escherichia coli

Flagellar brake protein YcgR in complex with c-di-GMP experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Flagellar brake protein YcgR in complex with c-di-GMP monomer, 29 kDa Escherichia coli protein
Buffer: 20 mM HEPES, 150mM NaCl, 10% glycerol,, pH: 7.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2016 Jan 4
Structural insights into the mechanism of c-di-GMP-bound YcgR regulating flagellar motility in Escherichia coli. J Biol Chem 295(3):808-821 (2020)
...Yang WS, Hong Y, Zhang Y, Wang DC, Li DF
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 44 nm3

SASDQF9 – GRP94 protein (Endoplasmin)

Endoplasmin experimental SAS data
Endoplasmin Kratky plot
Sample: Endoplasmin dimer, 169 kDa Mus musculus protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 11
Visualization of conformational transition of GRP94 in solution. Life Sci Alliance 7(2) (2024)
...Yang B
RgGuinier 5.6 nm
Dmax 24.0 nm
VolumePorod 260 nm3

SASDQG9 – GRP94 protein (Endoplasmin) bound to ADP

Endoplasmin experimental SAS data
Endoplasmin Kratky plot
Sample: Endoplasmin dimer, 169 kDa Mus musculus protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 11
Visualization of conformational transition of GRP94 in solution. Life Sci Alliance 7(2) (2024)
...Yang B
RgGuinier 5.7 nm
Dmax 24.0 nm
VolumePorod 274 nm3

SASDQH9 – GRP94 protein (Endoplasmin) bound to AMP-PNP

Endoplasmin experimental SAS data
Endoplasmin Kratky plot
Sample: Endoplasmin dimer, 169 kDa Mus musculus protein
Buffer: 25 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Jul 11
Visualization of conformational transition of GRP94 in solution. Life Sci Alliance 7(2) (2024)
...Yang B
RgGuinier 5.5 nm
Dmax 24.5 nm
VolumePorod 260 nm3

SASDQV9 – Nanobody1 in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Nanobody1 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody1 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody1 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
...Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 52 nm3

SASDQW9 – Nanobody2 in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Nanobody2 experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Nanobody2 Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Nanobody2 monomer, 14 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
...Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 2.0 nm
Dmax 9.0 nm
VolumePorod 24 nm3

SASDQX9 – Tandem nanobody in complex with the extracellular domain of human BCMA

Tumor necrosis factor receptor superfamily member 17Tandem nanobody experimental SAS data
Tumor necrosis factor receptor superfamily member 17 Tandem nanobody Kratky plot
Sample: Tumor necrosis factor receptor superfamily member 17 monomer, 6 kDa Homo sapiens protein
Tandem nanobody monomer, 27 kDa protein
Buffer: 20 mM Tris, 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2022 Aug 28
Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity. Signal Transduct Target Ther 8(1):445 (2023)
...Yang XN, Yang SS, Lyu YZ, Zhang B, Liu KW, Li N, Cui JC, Huang GX, Liu CL, Xu J, Mi JQ, Chen Z, Fan XH, Chen SJ, Chen S
RgGuinier 5.1 nm
Dmax 23.3 nm
VolumePorod 247 nm3

SASDRH5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Dec 12
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.3 nm
VolumePorod 12 nm3

SASDH38 – Wildtype preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-WT-apo

wildtype preQ1 riboswitch in Bacillus subtilis experimental SAS data
wildtype preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.6 nm
Dmax 10.5 nm
VolumePorod 20 nm3

SASDLD9 – 2-aminomuconic 6-semialdehyde dehydrogenase wild-type

2-aminomuconic 6-semialdehyde dehydrogenase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 2-aminomuconic 6-semialdehyde dehydrogenase tetramer, 215 kDa Pseudomonas sp. protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding. Environ Microbiol 24(7):2994-3012 (2022)
...Yang C, Liu G, Su D
RgGuinier 3.9 nm
Dmax 11.6 nm
VolumePorod 326 nm3

SASDRJ5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 26 nm3

SASDH48 – Wildtype preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-WT-holo

wildtype preQ1 riboswitch in Bacillus subtilis experimental SAS data
wildtype preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: wildtype preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.0 nm
Dmax 9.0 nm
VolumePorod 16 nm3

SASDLC9 – 2-aminomuconic 6-semialdehyde dehydrogenase double truncation mutant (Δ124-138; Δ477-491)

2-aminomuconic 6-semialdehyde dehydrogenase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 2-aminomuconic 6-semialdehyde dehydrogenase dimer, 101 kDa Pseudomonas sp. protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Feb 1
The tetrameric assembly of 2-aminomuconic 6-semialdehyde dehydrogenase is a functional requirement of cofactor NAD(+) binding. Environ Microbiol 24(7):2994-3012 (2022)
...Yang C, Liu G, Su D
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 117 nm3

SASDRG5 – A ssr1698 Dri1 hemoprotein, wild-type (apo) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDH58 – C15 deletion preQ1 riboswitch in Bacillus subtilis, apo-state - Bsu-C15d-apo

C15 deletion preQ1 riboswitch in Bacillus subtilis experimental SAS data
C15 deletion preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 17 nm3

SASDRK5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 10 nm3

SASDH68 – C15 deletion preQ1 riboswitch in Bacillus subtilis, holo-state - Bsu-C15d-holo

C15 deletion preQ1 riboswitch in Bacillus subtilis experimental SAS data
C15 deletion preQ1 riboswitch in Bacillus subtilis Kratky plot
Sample: C15 deletion preQ1 riboswitch in Bacillus subtilis monomer, 11 kDa Bacillus subtilis RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 2.0 nm
Dmax 8.8 nm
VolumePorod 14 nm3

SASDRL5 – A ssr1698 Dri1 hemoprotein, wild-type (Zn2+) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Sep 27
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.2 nm
VolumePorod 29 nm3

SASDH78 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state - Tte-WT-apo

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.6 nm
Dmax 6.7 nm
VolumePorod 12 nm3

SASDRM5 – A ssr1698 Dri1 hemoprotein, wild-type (Co2+) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 29 nm3

SASDH88 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state - Tte-WT-holo

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.6 nm
Dmax 7.2 nm
VolumePorod 13 nm3

SASDRF5 – A ssr1698 Dri1 hemoprotein, H21A variant

Ssr1698 protein (H21A) experimental SAS data
Sample: Ssr1698 protein (H21A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 10
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 12 nm3

SASDHB8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state - Tte-A14'-apo

A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.9 nm
Dmax 9.0 nm
VolumePorod 14 nm3

SASDHC8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state - Tte-A14'-holo

A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.8 nm
Dmax 12.0 nm
VolumePorod 16 nm3

SASDQS9 – A ssr1698 Dri1 hemoprotein, H21A variant + heme

Ssr1698 protein (H21A) experimental SAS data
Sample: Ssr1698 protein (H21A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Dec 7
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 28 nm3

SASDRE5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant

Ssr1698 protein (H79A:R90A) experimental SAS data
Sample: Ssr1698 protein (H79A:R90A) monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 12 nm3

SASDH98 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state high-temperature sampling - Tte-WT-apo-HT

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.7 nm
Dmax 7.0 nm
VolumePorod 11 nm3

SASDRD5 – A ssr1698 Dri1 hemoprotein, H79A:R90A variant + heme

Ssr1698 protein (H79A:R90A) experimental SAS data
Sample: Ssr1698 protein (H79A:R90A) dimer, 22 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Mar 30
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 2.0 nm
Dmax 6.3 nm
VolumePorod 28 nm3

SASDHA8 – Wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state high-temperature sampling - Tte-WT-holo-HT

wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: wildtype preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.5 nm
Dmax 6.5 nm
VolumePorod 10 nm3

SASDHD8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, apo-state high-temperature sampling - Tte-A14'-apo-HT

A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.8 nm
Dmax 7.5 nm
VolumePorod 13 nm3

SASDHE8 – A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis, holo-state high-temperature sampling - Tte-A14'-holo-HT

A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis experimental SAS data
A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis Kratky plot
Sample: A14 insertion preQ1 riboswitch in Thermoanaerobacter tengcongensis monomer, 11 kDa Caldanaerobacter subterraneus subsp. … RNA
Buffer: 50 mM potassium phosphate, 2 mM MgCl2, 50 mM KCl, pH: 6.5
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Mar 23
Hierarchical Conformational Dynamics Confers Thermal Adaptability to preQ1 RNA Riboswitches. J Mol Biol 432(16):4523-4543 (2020)
...Yang S, Dong X, Yang QF, Zhu YL, Xiao Y, Tang C
RgGuinier 1.9 nm
Dmax 8.8 nm
VolumePorod 14 nm3