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90 hits found for Hopkins

SASDMZ9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 57 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 5.4 nm
Dmax 20.3 nm
VolumePorod 93 nm3

SASDQZ9 – Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 0

Beclin-1 experimental SAS data
Beclin-1 Kratky plot
Sample: Beclin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
...Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 4.3 nm
Dmax 20.0 nm
VolumePorod 87 nm3

SASDN22 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 45 nm3

SASDR22 – Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1

Beclin-1 experimental SAS data
Beclin-1 amino acids 1-150 with two cysteine motifs, along with a C-terminal tyrosine analysed using EFA in BIOXTAS RAW software, component 1 Rg histogram
Sample: Beclin-1 monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
...Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 49 nm3

SASDN32 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 41 nm3

SASDN42 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domains 1 and 2 with maltoheptaose

Dockerin domain-containing protein experimental SAS data
Dockerin domain-containing protein Kratky plot
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 5.2 nm
Dmax 19.0 nm
VolumePorod 84 nm3

SASDPE2 – Starch adherence system protein 6 (Sas6)

Dockerin type I repeat experimental SAS data
MULTIFOXS model
Sample: Dockerin type I repeat monomer, 69 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch. Nat Struct Mol Biol (2024)
...Hopkins JB, Vander Kooi CW, Janeček Š, Ruotolo BT, Koropatkin NM
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 97 nm3

SASDPY2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein monomer

Replicase polyprotein 1ab experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1ab monomer, 31 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
...Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 50 nm3

SASDPZ2 – SARS-CoV-2 non-structural protein 7-8 (nsp7-8) polyprotein dimer

Replicase polyprotein 1ab experimental SAS data
Replicase polyprotein 1ab Kratky plot
Sample: Replicase polyprotein 1ab dimer, 63 kDa Severe acute respiratory … protein
Buffer: 50 mM Tris, 500 mM NaCl, 5% glycerol, and 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 15
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
...Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 110 nm3

SASDP23 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein monomer

Replicase polyprotein 1a experimental SAS data
DAMMIF model
Sample: Replicase polyprotein 1a monomer, 60 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
...Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 3.5 nm
Dmax 15.6 nm
VolumePorod 102 nm3

SASDP33 – SARS-CoV-2 non-structural protein 7-11 (nsp7-11) polyprotein dimer

Replicase polyprotein 1a experimental SAS data
Replicase polyprotein 1a Kratky plot
Sample: Replicase polyprotein 1a dimer, 119 kDa Severe acute respiratory … protein
Buffer: 20 mM HEPES, 10% glycerol, 500 mM NaCl, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 14
Biochemical and structural insights into SARS-CoV-2 polyprotein processing by Mpro. Sci Adv 8(49):eadd2191 (2022)
...Hopkins JB, Slack RL, Sarafianos SG, Ruiz FX, Griffin PR, Arnold E
RgGuinier 4.6 nm
Dmax 19.1 nm
VolumePorod 203 nm3

SASDP43 – Accumulation-association protein (Aap) Brpt5.5 monomer

Accumulation associated protein experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 monomer Rg histogram
Sample: Accumulation associated protein monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 16
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
...Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.7 nm
Dmax 55.8 nm

SASDP53 – Accumulation-association protein (Aap) Brpt5.5 5xH85A monomer

Accumulation associated protein (mutant) experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 5xH85A monomer Rg histogram
Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
...Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.1 nm
Dmax 57.2 nm

SASDP63 – Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer

Accumulation associated protein (mutant) experimental SAS data
Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer Rg histogram
Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
...Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 14.4 nm
Dmax 56.6 nm

SASDP73 – Accumulation-association protein (Aap) Brpt5.5 5xH85A dimer in the presence of ZnCl2

Accumulation associated protein (mutant) experimental SAS data
PYMOL model
Sample: Accumulation associated protein (mutant) dimer, 155 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
...Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 15.0 nm
Dmax 63.3 nm

SASDP83 – Accumulation-association protein (Aap) Brpt5.5 2xH85A tetramer in the presence of ZnCl2

Accumulation associated protein (mutant) experimental SAS data
PYMOL model
Sample: Accumulation associated protein (mutant) tetramer, 312 kDa Staphylococcus epidermidis (strain … protein
Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1
Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap. J Mol Biol :167708 (2022)
...Hopkins JB, Chatzimagas L, Hub JS, Herr AB
RgGuinier 15.7 nm
Dmax 70.0 nm

SASDP93 – K48-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 2.6 nm
Dmax 95.0 nm
VolumePorod 44 nm3

SASDPA3 – K63-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 13.0 nm
VolumePorod 44 nm3

SASDPB3 – M1-linked tetraubiquitin (Ub4)

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 46 nm3

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8
Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains. EMBO Rep :e55056 (2022)
...Hopkins JB, Ma W, Loh SN, Castañeda CA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 115 nm3

SASDRC3 – Homo-oligomeric mixture of human myelin protein zero Ig domain

Myelin protein P0 experimental SAS data
ALPHAFOLD model
Sample: Myelin protein P0 , 15 kDa Homo sapiens protein
Buffer: 50 mM NaCl, 1 mM EDTA, 20 mM TrisCl, pH: 7.6
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jul 21
Homomeric interactions of the MPZ Ig domain and their relation to Charcot-Marie-Tooth disease Brain (2023)
...Hopkins J, Ahern C, Shy M, Piper R
RgGuinier 2.9 nm
Dmax 17.0 nm

SASDRD3 – Homo-oligomeric mixture of human myelin protein zero Ig domain (W53A, R74A, D75R mutant)

Myelin protein P0 (W53A, R74A, D75R) experimental SAS data
ALPHAFOLD model
Sample: Myelin protein P0 (W53A, R74A, D75R) , 14 kDa Homo sapiens protein
Buffer: 50 mM NaCl, 1 mM EDTA, 20 mM TrisCl, pH: 7.6
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jul 14
Homomeric interactions of the MPZ Ig domain and their relation to Charcot-Marie-Tooth disease Brain (2023)
...Hopkins J, Ahern C, Shy M, Piper R
RgGuinier 1.9 nm
Dmax 7.0 nm

SASDMG4 – Arrestin-3 ERK2 fusion, monomer (Arrestin-3 fused to extracellular signal-regulated kinase 2)

Arrestin-3 fused to extracellular signal-regulated kinase 2 experimental SAS data
Arrestin-3 ERK2 fusion, monomer (Arrestin-3 fused to extracellular signal-regulated kinase 2) Rg histogram
Sample: Arrestin-3 fused to extracellular signal-regulated kinase 2 monomer, 89 kDa Bos taurus/Rattus norvegicus protein
Buffer: 20 mM MOPS pH 7.5, 150 mM NaCl, 1 mM TCEP, and 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 23
The two non-visual arrestins engage ERK2 differently. J Mol Biol :167465 (2022)
...Hopkins J, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Young Chung K, Klug CS, Gurevich VV, Iverson TM
RgGuinier 3.6 nm
Dmax 14.0 nm

SASDMH4 – Arrestin-3 ERK2 fusion, dimer (Arrestin-3 fused to extracellular signal-regulated kinase 2)

Arrestin-3 fused to extracellular signal-regulated kinase 2 experimental SAS data
Arrestin-3 fused to extracellular signal-regulated kinase 2 Kratky plot
Sample: Arrestin-3 fused to extracellular signal-regulated kinase 2 dimer, 178 kDa Bos taurus/Rattus norvegicus protein
Buffer: 20 mM MOPS pH 7.5, 150 mM NaCl, 1 mM TCEP, and 5% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Mar 23
The two non-visual arrestins engage ERK2 differently. J Mol Biol :167465 (2022)
...Hopkins J, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Young Chung K, Klug CS, Gurevich VV, Iverson TM
RgGuinier 4.3 nm
Dmax 16.0 nm

SASDPP4 – Consensus SAXS Profile - Ribonuclease A

Ribonuclease pancreatic experimental SAS data
DAMMIN model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 18 nm3

SASDPQ4 – Consensus SAXS Profile - Urate Oxidase

Uricase experimental SAS data
DAMMIN model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.2 nm
Dmax 9.2 nm
VolumePorod 220 nm3

SASDPR4 – Consensus SAXS Profile - Xylose Isomerase

Xylose isomerase experimental SAS data
DAMMIN model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: ConsensusBuffer_50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 243 nm3

SASDPS4 – Consensus SAXS Profile - Xylanase

Endo-1,4-beta-xylanase experimental SAS data
DAMMIN model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.6 nm
Dmax 5.1 nm
VolumePorod 27 nm3

SASDPT4 – Consensus SAXS Profile - Lysozyme

Lysozyme C experimental SAS data
DAMMIN model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 19 nm3

SASDPU4 – Consensus SANS Profile - Ribonuclease A in 100% v/v D2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.4 nm
Dmax 4.4 nm

SASDPV4 – Consensus SANS Profile - Lysozyme in 100% v/v D2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.2 nm
Dmax 3.8 nm

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.5 nm
Dmax 4.4 nm

SASDPX4 – Consensus SANS Profile - Urate oxidase in 100% v/v D2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.1 nm
Dmax 9.3 nm

SASDPY4 – Consensus SANS Profile - Xylose isomerase in 100% v/v D2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.1 nm
Dmax 9.5 nm

SASDPZ4 – Consensus SANS Profile - Lysozyme in H2O buffer

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.4 nm
Dmax 4.8 nm

SASDP25 – Consensus SANS Profile - Ribonuclease A in H2O buffer

Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.5 nm
Dmax 4.1 nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 1.6 nm
Dmax 4.3 nm

SASDP45 – Consensus SANS Profile - Urate oxidase in H2O buffer

Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.2 nm
Dmax 9.1 nm

SASDP55 – Consensus SANS Profile - Xylose isomerase in H2O buffer

Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 24
A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
...Hopkins J, Huang Q, Hub J, Hura G, Irving T, Jeffries C, Jeong C, Kirby N, Krueger S, Martel A, Matsui T, Li N, Pérez J, Porcar L, Prangé T, Rajkovic I, Rocco M, Rosenberg D, Ryan T, Seifert S, Sekigu...
RgGuinier 3.3 nm
Dmax 9.7 nm

SASDSH5 – UDP-glycosyltransferase 202A2 from Tetranychus urticae

UDP-glycosyltransferase 202A2 experimental SAS data
MULTIFOXS model
Sample: UDP-glycosyltransferase 202A2 monomer, 53 kDa Tetranychus urticae protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 17
Structural and functional studies reveal the molecular basis of substrate promiscuity of a glycosyltransferase originating from a major agricultural pest Journal of Biological Chemistry :105421 (2023)
...Hopkins J, Van Leeuwen T, Grbic M, Grbic V, Borowski T, Chruszcz M
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDHT5 – Human Carbonic Anhydrase IX

Carbonic anhydrase 9 experimental SAS data
Human Carbonic Anhydrase IX Rg histogram
Sample: Carbonic anhydrase 9 monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Nov 16
Biophysical Characterization of Cancer-Related Carbonic Anhydrase IX. Int J Mol Sci 21(15) (2020)
...Hopkins JB, Knecht W, McKenna R, Fisher SZ
RgGuinier 2.5 nm
Dmax 8.5 nm
VolumePorod 71 nm3

SASDPU6 – Duplex-G4-duplex DNA

Duplex-G4-duplex experimental SAS data
AMBER model
Sample: Duplex-G4-duplex monomer, 29 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Jun 16
Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 Å resolution with cryo-EM. Nucleic Acids Res (2023)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 59 nm3

SASDP28 – Growth factor receptor-bound protein 2 (GRB2) - wild-type monomer

Growth factor receptor-bound protein 2 experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 40 nm3

SASDP38 – Growth factor receptor-bound protein 2 (GRB2) - wild-type dimer

Growth factor receptor-bound protein 2 experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 110 nm3

SASDP48 – Growth factor receptor-bound protein 2 (GRB2) - N188D/N214D mutant monomer

Growth factor receptor-bound protein 2 (N188D, N214D) experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 (N188D, N214D) monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 39 nm3

SASDP58 – Growth factor receptor-bound protein 2 (GRB2) - N188D/N214D mutant dimer

Growth factor receptor-bound protein 2 (N188D, N214D) experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 (N188D, N214D) dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 140 nm3

SASDP68 – Growth factor receptor-bound protein 2 (GRB2) - V123D mutant monomer

Growth factor receptor-bound protein 2 (V123D) experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 (V123D) monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 2.6 nm
Dmax 10.0 nm
VolumePorod 45 nm3

SASDP78 – Growth factor receptor-bound protein 2 (GRB2) - V122P/V123P mutant dimer

Growth factor receptor-bound protein 2 (V122P, V123P) experimental SAS data
PYMOL model
Sample: Growth factor receptor-bound protein 2 (V122P, V123P) dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 31
GRB2 dimerization mediated by SH2 domain-swapping is critical for T cell signaling and cytokine production. Sci Rep 13(1):3505 (2023)
...Hopkins JB, Chakravarthy S, Gakhar L, Schnicker NJ, Houtman JCD
RgGuinier 3.7 nm
Dmax 20.4 nm
VolumePorod 85 nm3

SASDNF8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer

Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.4 nm
Dmax 18.4 nm
VolumePorod 264 nm3

SASDNG8 – LARGE xylosyl- and glucuronyltransferase 1 (LARGE1dTM) dimer treated with PNGase F enzyme

Xylosyl- and glucuronyltransferase LARGE1 experimental SAS data
DAMMIF model
Sample: Xylosyl- and glucuronyltransferase LARGE1 dimer, 179 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.3 nm
Dmax 18.0 nm

SASDNH8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer

Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.2 nm
Dmax 16.5 nm

SASDNJ8 – Xylosyl- and glucuronyltransferase LARGE2 (LARGE2dTM) dimer treated with PNGase F enzyme

Xylosyl- and glucuronyltransferase LARGE2 experimental SAS data
DAMMIN model
Sample: Xylosyl- and glucuronyltransferase LARGE2 dimer, 168 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Mar 18
LARGE1 Processively Polymerizes Matriglycan Using Active Sites on Alternate Protomers SSRN Electronic Journal ()
...Hopkins J, Watkins M, Chakravarthy S, Davulcu O, Anderson M, Venzke D, Campbell K
RgGuinier 4.1 nm
Dmax 15.9 nm
VolumePorod 231 nm3

SASDLM8 – Human apo Nocturnin - Extended

Nocturnin experimental SAS data
Human apo Nocturnin - Extended Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
...Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.9 nm
Dmax 12.3 nm
VolumePorod 85 nm3

SASDLN8 – Human apo Nocturnin - Compact

Nocturnin experimental SAS data
Human apo Nocturnin - Compact Rg histogram
Sample: Nocturnin monomer, 41 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
...Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.4 nm
Dmax 9.6 nm
VolumePorod 76 nm3

SASDLP8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Extended

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Extended Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
...Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 72 nm3

SASDLQ8 – Human apo Nocturnin - Deletion construct (Δ107-120) - Compact

Nocturnin - Deletion construct - Δ107-120 experimental SAS data
Human apo Nocturnin - Deletion construct (Δ107-120) - Compact Rg histogram
Sample: Nocturnin - Deletion construct - Δ107-120 monomer, 40 kDa protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
...Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 80 nm3

SASDLR8 – Human apo Nocturnin - N terminus truncated (118-NOCT)

Nocturnin - N terminus truncated (118-NOCT) experimental SAS data
EOM/RANCH model
Sample: Nocturnin - N terminus truncated (118-NOCT) monomer, 36 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM KCl, 10% glycerol, 5 mM MgCl2, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Feb 7
The Disordered Amino Terminus of the Circadian Enzyme Nocturnin Modulates Its NADP(H) Phosphatase Activity by Changing Protein Dynamics. Biochemistry (2022)
...Hopkins JB, Joachimiak LA, Green CB
RgGuinier 2.3 nm
Dmax 10.3 nm
VolumePorod 63 nm3

SASDQJ9 – An assembly between C-terminal truncated Netrin UNC-6 and the extracellular domain of Netrin receptor UNC-5 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD complex)

Netrin unc-6Isoform a of Netrin receptor unc-5Heparin, porcine intestinal mucosa experimental SAS data
Netrin unc-6 Isoform a of Netrin receptor unc-5 Heparin, porcine intestinal mucosa Kratky plot
Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein
Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein
Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus
Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 18
Structural insights into the formation of repulsive netrin guidance complexes. Sci Adv 10(7):eadj8083 (2024)
...Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
RgGuinier 10.4 nm
Dmax 37.3 nm
VolumePorod 2156 nm3

SASDQK9 – An assembly between C-terminal truncated Netrin UNC-6, the extracellular domains of Netrin receptor UNC-5 and UNC-40 with porcine intestinal mucosa heparin (UNC-6ΔC-heparin-UNC-5 ECD-UNC-40 ECD comple...

Netrin unc-6Isoform a of Netrin receptor unc-5Heparin, porcine intestinal mucosaNetrin receptor unc-40 experimental SAS data
Netrin unc-6 Isoform a of Netrin receptor unc-5 Heparin, porcine intestinal mucosa Netrin receptor unc-40 Kratky plot
Sample: Netrin unc-6 monomer, 52 kDa Caenorhabditis elegans protein
Isoform a of Netrin receptor unc-5 monomer, 38 kDa Caenorhabditis elegans protein
Heparin, porcine intestinal mucosa monomer, 15 kDa Sus scrofa domesticus
Netrin receptor unc-40 monomer, 118 kDa Caenorhabditis elegans protein
Buffer: 10 mM HEPES pH 7.2, 150 mM NaCl, 100 mM MgSO4, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Dec 11
Structural insights into the formation of repulsive netrin guidance complexes. Sci Adv 10(7):eadj8083 (2024)
...Hopkins JB, Mendoza JL, Meijers R, Shen K, Özkan E
RgGuinier 10.6 nm
Dmax 36.5 nm
VolumePorod 3410 nm3

SASDMX9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 1

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 2.1 nm
Dmax 10.3 nm
VolumePorod 42 nm3

SASDMY9 – Dockerin domain-containing protein, starch adherence system 20 (Sas20), domain 2

Dockerin domain-containing protein experimental SAS data
MULTIFOXS model
Sample: Dockerin domain-containing protein monomer, 26 kDa Ruminococcus bromii protein
Buffer: phosphate buffered saline, 1 mM TCEP, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Mar 28
Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome Journal of Biological Chemistry :101896 (2022)
...Hopkins J, Koropatkin N
RgGuinier 2.3 nm
Dmax 7.8 nm
VolumePorod 35 nm3

SASDQY9 – Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine, along with a C-terminal tyrosine

Beclin-1 (C18S, C21S, A103V, C137S, C140S) experimental SAS data
Beclin-1 amino acids 1-150 with cysteine motif residues mutated to serine, along with a C-terminal tyrosine Rg histogram
Sample: Beclin-1 (C18S, C21S, A103V, C137S, C140S) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM Tris, 300 mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 12
Invariant BECN1 CXXC motifs bind Zn(2+) and regulate structure and function of the BECN1 intrinsically disordered region. Autophagy :1-17 (2023)
...Hopkins JB, Ugrinov A, Chakravarthy S, Colbert CL, Sinha SC
RgGuinier 4.1 nm
Dmax 19.3 nm
VolumePorod 57 nm3

SASDTK2 – V2 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V2 variant of the low complexity domain of hnRNPA1 experimental SAS data
V2 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V2 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa synthetic construct protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
RgGuinier 2.3 nm
Dmax 8.3 nm
VolumePorod 19 nm3

SASDMJ6 – cMyc-8

cMyc promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cMyc promoter 8-tract G-quadruplex monomer, 11 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 13 nm3

SASDHY9 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-408): DHPH

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein experimental SAS data
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-408): DHPH Rg histogram
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 43 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300mM NaCl, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding. J Biol Chem (2020)
...Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG
RgGuinier 2.8 nm
Dmax 10.5 nm
VolumePorod 64 nm3

SASDTL2 – V3 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V3 variant of the low complexity domain of hnRNPA1 experimental SAS data
V3 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V3 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
RgGuinier 2.3 nm
Dmax 9.3 nm
VolumePorod 18 nm3

SASDMM6 – cMyc-12

cMyc promoter 12-tract G-quadruplex experimental SAS data
cMyc promoter 12-tract G-quadruplex Kratky plot
Sample: cMyc promoter 12-tract G-quadruplex monomer, 23 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 22
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.3 nm
Dmax 8.4 nm
VolumePorod 29 nm3

SASDHW9 – Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): DHPHDEP1

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein experimental SAS data
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): DHPHDEP1 Rg histogram
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding. J Biol Chem (2020)
...Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG
RgGuinier 3.0 nm
Dmax 10.4 nm
VolumePorod 77 nm3

SASDTM2 – V4 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V4 variant of the low complexity domain of hnRNPA1 experimental SAS data
V4 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V4 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
RgGuinier 2.4 nm
Dmax 9.9 nm
VolumePorod 19 nm3

SASDM36 – c-Myc12 DnaseI Treated G-quadruplex

c-Myc 12-tract G-quadruplex monomer Dnase I treated experimental SAS data
CHIMERA model
Sample: c-Myc 12-tract G-quadruplex monomer Dnase I treated monomer, 22 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 25 nm3

SASDHX9 – Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 experimental SAS data
Phosphorylated phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (amino acids 38-499): pDHPHDEP1 Rg histogram
Sample: Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 300 mM NaCl, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity andcontributes to membrane binding. J Biol Chem (2020)
...Hopkins JB, Plescia CB, Allgood SR, Kane MA, Cash JN, Stahelin RV, Tesmer JJG
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 75 nm3

SASDTN2 – V5 variant of the low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

V5 variant of the low complexity domain of hnRNPA1 experimental SAS data
V5 variant of the low complexity domain of hnRNPA1 Kratky plot
Sample: V5 variant of the low complexity domain of hnRNPA1 monomer, 13 kDa protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 19 nm3

SASDMK6 – cKit-8

cKit promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cKit promoter 8-tract G-quadruplex monomer, 12 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.5 nm
Dmax 4.7 nm
VolumePorod 15 nm3

SASDTJ2 – Low complexity domain of hnRNPA1 (Heterogeneous nuclear ribonucleoprotein A1)

Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 experimental SAS data
Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 Kratky plot
Sample: Isoform A1-A of Heterogeneous nuclear ribonucleoprotein A1 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 19
Design of intrinsically disordered protein variants with diverse structural properties Science Advances 10(35) (2024)
...Hopkins J, Grace C, Mittag T, Lindorff-Larsen K
RgGuinier 2.4 nm
Dmax 8.0 nm
VolumePorod 19 nm3

SASDMN6 – cKit-12

cKit promoter 12-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: cKit promoter 12-tract G-quadruplex monomer, 20 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 22
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 2.5 nm
Dmax 9.0 nm
VolumePorod 26 nm3

SASDM46 – c-Kit12 DNase I Digest

c-Kit 12-tract G-quadruplex monomer Dnase I treated experimental SAS data
c-Kit 12-tract G-quadruplex monomer Dnase I treated Kratky plot
Sample: c-Kit 12-tract G-quadruplex monomer Dnase I treated monomer, 20 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Sep 29
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.6 nm
Dmax 5.7 nm
VolumePorod 16 nm3

SASDML6 – kRas-8

kRas promoter 8-tract G-quadruplex experimental SAS data
CHIMERA model
Sample: kRas promoter 8-tract G-quadruplex monomer, 14 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 19
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.7 nm
Dmax 5.6 nm
VolumePorod 16 nm3

SASDM56 – 2KZD

TERT promoter G-quadruplex antiparallel experimental SAS data
CHIMERA model
Sample: TERT promoter G-quadruplex antiparallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 13
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.6 nm
VolumePorod 7 nm3

SASDM76 – 201D

DNA oligonucleotide G4(T4G4)3 experimental SAS data
CHIMERA model
Sample: DNA oligonucleotide G4(T4G4)3 monomer, 9 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 12
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.1 nm
VolumePorod 10 nm3

SASDM86 – 6GH0

cKIT 2-tetrad promoter GQ antiparallel experimental SAS data
CHIMERA model
Sample: cKIT 2-tetrad promoter GQ antiparallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.0 nm
Dmax 3.1 nm
VolumePorod 8 nm3

SASDM96 – 2GKU

human telomere 24mer hybrid-1 experimental SAS data
CHIMERA model
Sample: human telomere 24mer hybrid-1 monomer, 8 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.8 nm
VolumePorod 8 nm3

SASDMA6 – 5I2V

kRas promoter GQ hybrid experimental SAS data
CHIMERA model
Sample: kRas promoter GQ hybrid monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.9 nm
VolumePorod 10 nm3

SASDMB6 – 6L92

WNT promoter GQ hybrid form experimental SAS data
CHIMERA model
Sample: WNT promoter GQ hybrid form monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 21
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.9 nm
VolumePorod 8 nm3

SASDMC6 – 2KQG

cKit promoter GQ parallel experimental SAS data
CHIMERA model
Sample: cKit promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.6 nm
Dmax 3.2 nm
VolumePorod 14 nm3

SASDMD6 – 2LBY

cMyc promoter GQ parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter GQ parallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.2 nm
Dmax 3.9 nm
VolumePorod 7 nm3

SASDME6 – 6NEB

cMyc promoter GQ 1:6:1 loop parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter GQ 1:6:1 loop parallel monomer, 9 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.4 nm
Dmax 4.5 nm
VolumePorod 10 nm3

SASDMF6 – 1XAV

cMyc promoter modified GQ parallel experimental SAS data
CHIMERA model
Sample: cMyc promoter modified GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.1 nm
VolumePorod 9 nm3

SASDM66 – 2KZE

TERT promoter G-quadruplex parallel experimental SAS data
CHIMERA model
Sample: TERT promoter G-quadruplex parallel monomer, 6 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Aug 11
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 4.2 nm
VolumePorod 8 nm3

SASDMG6 – 5CMX

two-tetrad antiparallel GQ with duplex junction experimental SAS data
CHIMERA model
Sample: two-tetrad antiparallel GQ with duplex junction monomer, 10 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 3
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.5 nm
Dmax 5.2 nm
VolumePorod 12 nm3

SASDMH6 – 2M27

VEGF promoter GQ parallel experimental SAS data
CHIMERA model
Sample: VEGF promoter GQ parallel monomer, 7 kDa synthetic construct DNA
Buffer: 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH: 7.2
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 20
Long promoter sequences form higher-order G-quadruplexes: an integrative structural biology study of c-Myc, k-Ras and c-Kit promoter sequences. Nucleic Acids Res (2022)
...Hopkins JB, Chaires JB, Trent JO
RgGuinier 1.3 nm
Dmax 3.8 nm
VolumePorod 8 nm3