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112 hits found for Van

SASDC82 – Rab family protein CtRoco nucleotide free

Rab family protein experimental SAS data
DAMMIN model
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.0 nm
Dmax 18.4 nm
VolumePorod 440 nm3

SASDC92 – Rab family protein CtRoco GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 6.2 nm
Dmax 25.3 nm
VolumePorod 354 nm3

SASDCA2 – Rab family protein CtRoco GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.9 nm
Dmax 22.4 nm
VolumePorod 491 nm3

SASDCB2 – Rab family protein CtRoc-COR nucleotide free

Rab family protein experimental SAS data
DAMMIN model
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.4 nm
Dmax 10.0 nm
VolumePorod 211 nm3

SASDCC2 – Rab family protein CtRoc-COR GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µ M GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.5 nm
Dmax 10.0 nm
VolumePorod 197 nm3

SASDCD2 – Rab family protein CtRoc-COR GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.4 nm
Dmax 10.5 nm
VolumePorod 154 nm3

SASDCE2 – Rab family protein CtRoco L487A GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.9 nm
Dmax 21.8 nm
VolumePorod 469 nm3

SASDCF2 – Rab family protein CtRoco L487A GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 6.0 nm
Dmax 23.0 nm
VolumePorod 436 nm3

SASDCG2 – Rab family protein CtRoco L487A

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
...Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.0 nm
Dmax 18.4 nm
VolumePorod 447 nm3

SASDFA3 – Human ATP-citrate synthase (ACLY) full length in HBS

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 19.0 nm
VolumePorod 765 nm3

SASDNA3 – Association of the CPAP N-termini coiled-coil domain with the CP110 C-termini coiled-coil region

centrosomal-P4.1-associated-proteinCentriolar coiled-coil protein of 110 kDa experimental SAS data
DAMMIF model
Sample: centrosomal-P4.1-associated-protein monomer, 12 kDa Homo sapiens protein
Centriolar coiled-coil protein of 110 kDa dimer, 20 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 23
Microtubule plus-end regulation by centriolar cap proteins (2021)
...van Hoorn C, Wu J, Andrea N, Hua S, Jiang K, Vakonakis I, Potocnjak M, Herzog F, Gigant B, Gudimchuk N, Stecker K, Dogterom M, Steinmetz M, Akhmanova A
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 35 nm3

SASDFB3 – Human ATP-citrate synthers (ACLY) full length in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.2 nm
Dmax 19.0 nm
VolumePorod 787 nm3

SASDNB3 – Homodimerisation of the CP110 C-terminus coiled-coil domain

Centriolar coiled-coil protein of 110 kDa experimental SAS data
DAMMIF model
Sample: Centriolar coiled-coil protein of 110 kDa dimer, 20 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 23
Microtubule plus-end regulation by centriolar cap proteins (2021)
...van Hoorn C, Wu J, Andrea N, Hua S, Jiang K, Vakonakis I, Potocnjak M, Herzog F, Gigant B, Gudimchuk N, Stecker K, Dogterom M, Steinmetz M, Akhmanova A
RgGuinier 3.5 nm
Dmax 12.5 nm
VolumePorod 33 nm3

SASDFC3 – Human ATP-citrate synthase (ACLY) full length in HBS + Citrate + Coenzyme-A

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.9 nm
Dmax 17.0 nm
VolumePorod 775 nm3

SASDLF3 – Staphylococcal immunoglobulin-binding protein bound to complement component C3

Protein A experimental SAS data
DAMMIN model
Sample: Protein A monomer, 28 kDa Staphylococcus aureus protein
Buffer: 10 mM HEPES, pH 7.2, 3 mM EDTA, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 12
A structural basis for Staphylococcal complement subversion: X-ray structure of the complement-binding domain of Staphylococcus aureus protein Sbi in complex with ligand C3d Molecular Immunology 48(4):452-462 (2011)
...van den Elsen J
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 81 nm3

SASDLG3 – Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Regulatory protein E2 , 1037 kDa Human papillomavirus type … protein
Buffer: 50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Jul 13
The catalytic core of an archaeal 2-oxoacid dehydrogenase multienzyme complex is a 42-mer protein assembly FEBS Journal 279(5):713-723 (2012)
...van den Elsen J
RgGuinier 8.8 nm
Dmax 22.0 nm
VolumePorod 2473 nm3

SASDLH3 – Truncated Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Regulatory protein E2 trimer, 73 kDa Human papillomavirus type … protein
Buffer: 50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 27
Why are the 2-oxoacid dehydrogenase complexes so large? Generation of an active trimeric complex Biochemical Journal 463(3):405-412 (2014)
...van den Elsen J, Danson M
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 149 nm3

SASDPK3 – Vibrio cholerae ParD2:ParE2 complex bound to 21-bp DNA operator box

Antitoxin ParDToxin21-bp DNA operator fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
21-bp DNA operator fragment monomer, 13 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.2 nm
Dmax 10.0 nm
VolumePorod 140 nm3

SASDPL3 – Vibrio cholerae ParD2:ParE2 complex bound to 31-bp DNA operator box

Antitoxin ParDToxin31-bp DNA operator box experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
31-bp DNA operator box monomer, 19 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 160 nm3

SASDPM3 – Vibrio cholerae ParD2:ParE2 complex bound to 33-bp DNA operator fragment

Antitoxin ParDToxin33-bp DNA operator fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
33-bp DNA operator fragment monomer, 20 kDa Vibrio cholerae O1 DNA
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.2 nm
Dmax 10.0 nm
VolumePorod 150 nm3

SASDPN3 – Vibrio cholerae ParD2:ParE2 antitoxin:toxin complex

Antitoxin ParDToxin experimental SAS data
CUSTOM IN-HOUSE model
Sample: Antitoxin ParD hexamer, 54 kDa Vibrio cholerae serotype … protein
Toxin , 25 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 6
Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Sci Adv 10(1):eadj2403 (2024)
...Van Dyck J, Muruganandam G, Sobott F, Charlier D, Loris R
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 140 nm3

SASDAV3 – Geminin:Cdt1 2:1 heterotrimer

GemininDNA replication factor Cdt1 experimental SAS data
CRYSOL model
Sample: Geminin dimer, 47 kDa Homo sapiens protein
DNA replication factor Cdt1 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Aug 13
Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. Proc Natl Acad Sci U S A 106(47):19807-12 (2009)
...van Gerwen S, Fish A, Petoukhov MV, Iliou MS, Lygerou Z, Medema RH, Blow JJ, Svergun DI, Taraviras S, Perrakis A
RgGuinier 2.9 nm
Dmax 10.0 nm
VolumePorod 70 nm3

SASDAW3 – Geminin:Cdt1 4:2 heterohexamer

GemininDNA replication factor Cdt1 experimental SAS data
CRYSOL model
Sample: Geminin dimer, 47 kDa Homo sapiens protein
DNA replication factor Cdt1 monomer, 60 kDa Homo sapiens protein
Buffer: 25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Oct 5
Quaternary structure of the human Cdt1-Geminin complex regulates DNA replication licensing. Proc Natl Acad Sci U S A 106(47):19807-12 (2009)
...van Gerwen S, Fish A, Petoukhov MV, Iliou MS, Lygerou Z, Medema RH, Blow JJ, Svergun DI, Taraviras S, Perrakis A
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 120 nm3

SASDA94 – Der p21

Der p21, allegren experimental SAS data
DAMMIN model
Sample: Der p21, allegren dimer, 26 kDa Escherichia coli protein
Buffer: 10 mM Hepes , pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jul 24
Characterization of Der p 21, a new important allergen derived from the gut of house dust mites. Allergy 63(6):758-67 (2008)
...van Hage M, Vieths S, Spitzauer S, Horak F, Svergun DI, Konarev PV, Valent P, Thalhamer J, Keller W, Valenta R, Vrtala S
RgGuinier 2.7 nm
Dmax 8.5 nm
VolumePorod 50 nm3

SASDAA4 – Full length GbpA

Full length GbpA experimental SAS data
GASBOR model
Sample: Full length GbpA monomer, 54 kDa Vibrio cholerae protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 16
The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
...van Aalten DM
RgGuinier 3.9 nm
Dmax 14.5 nm
VolumePorod 100 nm3

SASDAB4 – Truncated GbpA

Truncated GbpA experimental SAS data
GASBOR model
Sample: Truncated GbpA monomer, 44 kDa Vibrio cholerae protein
Buffer: 25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 16
The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces. PLoS Pathog 8(1):e1002373 (2012)
...van Aalten DM
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 80 nm3

SASDL35 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (12 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 23.3 nm
VolumePorod 320 nm3

SASDL45 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (6 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 6.6 nm
Dmax 22.9 nm
VolumePorod 325 nm3

SASDL55 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (4 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 6.4 nm
Dmax 21.9 nm
VolumePorod 315 nm3

SASDL65 – Schizosaccharomyces pombe SMN(GCN4IL) Chimera•Gemin2 complex (3 mg/mL)

Survival motor neuron-like protein 1Survival of motor neuron protein-interacting protein yip11Gcn4p experimental SAS data
Survival motor neuron-like protein 1 Survival of motor neuron protein-interacting protein yip11 Gcn4p Kratky plot
Sample: Survival motor neuron-like protein 1 dimer, 26 kDa Schizosaccharomyces pombe (strain … protein
Survival of motor neuron protein-interacting protein yip11 dimer, 54 kDa Schizosaccharomyces pombe (strain … protein
Gcn4p dimer, 8 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 150 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at X21, National Synchrotron Light Source (NSLS) on 2011 Oct 21
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 6.3 nm
Dmax 19.9 nm
VolumePorod 315 nm3

SASDL75 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Tetramer analyzed by SVD-EFA SEC-SAXS

Survival motor neuron proteinMaltose/maltodextrin-binding periplasmic protein experimental SAS data
Survival motor neuron protein Maltose/maltodextrin-binding periplasmic protein Kratky plot
Sample: Survival motor neuron protein tetramer, 15 kDa Drosophila melanogaster protein
Maltose/maltodextrin-binding periplasmic protein tetramer, 174 kDa Escherichia coli (strain … protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...vanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 3.9 nm
Dmax 11.3 nm
VolumePorod 340 nm3

SASDL85 – Maltose Binding Protein (MBP) fused to Drosophila Melanogaster SMN186–220: Octamer analyzed by SVD-EFA SEC-SAXS

Maltose/maltodextrin-binding periplasmic proteinSurvival motor neuron protein experimental SAS data
Maltose/maltodextrin-binding periplasmic protein Survival motor neuron protein Kratky plot
Sample: Maltose/maltodextrin-binding periplasmic protein octamer, 347 kDa Escherichia coli (strain … protein
Survival motor neuron protein octamer, 31 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...vanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 5.6 nm
Dmax 17.9 nm
VolumePorod 800 nm3

SASDL95 – Drosophila Melanogaster Gemin2-SMN: Octamer analyzed by SVD-EFA SEC-SAXS

Protein Gemin2Survival motor neuron protein experimental SAS data
Protein Gemin2 Survival motor neuron protein Kratky plot
Sample: Protein Gemin2 octamer, 230 kDa Drosophila melanogaster protein
Survival motor neuron protein octamer, 197 kDa Drosophila melanogaster protein
Buffer: 20 mM Na/KPO4 pH 7.0, 300 mM NaCl, 1 mM DTT, pH: 7
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...vanced Light Source (ALS) on 2018 Dec 19
Assembly of higher-order SMN oligomers is essential for metazoan viability and requires an exposed structural motif present in the YG zipper dimer. Nucleic Acids Res 49(13):7644-7664 (2021)
...Van Duyne GD, Matera AG
RgGuinier 7.8 nm
Dmax 28.7 nm
VolumePorod 2660 nm3

SASDEG5 – apo human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 300 nm3

SASDEH5 – AMP/fumarate-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 256 nm3

SASDSH5 – UDP-glycosyltransferase 202A2 from Tetranychus urticae

UDP-glycosyltransferase 202A2 experimental SAS data
MULTIFOXS model
Sample: UDP-glycosyltransferase 202A2 monomer, 53 kDa Tetranychus urticae protein
Buffer: 20 mM sodium phosphate, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at BioCAT 18ID, ...vanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 17
Structural and functional studies reveal the molecular basis of substrate promiscuity of a glycosyltransferase originating from a major agricultural pest Journal of Biological Chemistry :105421 (2023)
...Van Leeuwen T, Grbic M, Grbic V, Borowski T, Chruszcz M
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDEJ5 – AICAR/fumarate-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 250 nm3

SASDJJ5 – Histone H2A:H2B:H3:H4 complex with aprataxin and polynucleotide kinase like factor (APLF) acidic domain

Aprataxin and PNK-like factor (acidic domain)Histone H2AHistone H2BHistone H3Histone H4 experimental SAS data
DAMMIF model
Sample: Aprataxin and PNK-like factor (acidic domain) dimer, 15 kDa Homo sapiens protein
Histone H2A dimer, 26 kDa Drosophila melanogaster protein
Histone H2B dimer, 27 kDa Drosophila melanogaster protein
Histone H3 dimer, 30 kDa Drosophila melanogaster protein
Histone H4 dimer, 23 kDa Drosophila melanogaster protein
Buffer: 25 mM NaPi, 300 mM NaCl, 3% v/v glycerol, 1 mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 10
Chaperoning of the histone octamer by the acidic domain of DNA repair factor APLF. Sci Adv 8(30):eabo0517 (2022)
...van Attikum H, Boelens R, Sixma TK, Mattiroli F, van Ingen H
RgGuinier 3.7 nm
Dmax 10.8 nm
VolumePorod 260 nm3

SASDEK5 – AMP-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 292 nm3

SASDEL5 – AICAR-bound human adenylosuccinate lyase (ADSL)

Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 291 nm3

SASDEM5 – apo neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 304 nm3

SASDEN5 – AMP/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 258 nm3

SASDEP5 – AMP-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
Adenylosuccinate lyase Kratky plot
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 294 nm3

SASDEQ5 – AICAR-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
Adenylosuccinate lyase Kratky plot
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 292 nm3

SASDER5 – AICAR/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 252 nm3

SASDGS5 – MvaT (low salt data set)

MvaT(mutant) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: MvaT(mutant) dimer, 28 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Bis-Tris 50 mM KCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2018 May 11
Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res (2020)
...van Schaik P, Ubbink M, Dame RT
RgGuinier 3.6 nm
Dmax 14.7 nm
VolumePorod 47 nm3

SASDGT5 – MvaT (high salt data set)

MvaT(mutant) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: MvaT(mutant) dimer, 28 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Bis-Tris 300 mM KCl, pH: 6
Experiment: SAXS data collected at BM29, ESRF on 2018 May 11
Structural basis for osmotic regulation of the DNA binding properties of H-NS proteins. Nucleic Acids Res (2020)
...van Schaik P, Ubbink M, Dame RT
RgGuinier 3.8 nm
Dmax 15.8 nm
VolumePorod 50 nm3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS

ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 17.5 nm
VolumePorod 738 nm3

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate

ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 17.5 nm
VolumePorod 747 nm3

SASDE56 – Human ATP-citrate synthase (ACLY) in HBS + Citrate + Coenzyme-A

ATP-citrate synthase experimental SAS data
SASREF CV model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 5
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.8 nm
Dmax 16.5 nm
VolumePorod 709 nm3

SASDE66 – C. limicola ATP-citrate lyase (ACL) in HBS

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 20.0 nm
VolumePorod 666 nm3

SASDE76 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 672 nm3

SASDE86 – C. limicola ATP-citrate lyase (ACL) in HBS + Citrate + Coenzyme-A

ATP-citrate lyase experimental SAS data
ATP-citrate lyase Kratky plot
Sample: ATP-citrate lyase tetramer, 429 kDa Chlorobium limicola protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 4
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
...Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.7 nm
Dmax 17.5 nm
VolumePorod 791 nm3

SASDF86 – Human Galectin-10 (Tyr69Glu mutant)

Galectin-10 Tyr69Glu experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Galectin-10 Tyr69Glu dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM Hepes 150 NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2018 Feb 4
Protein crystallization promotes type 2 immunity and is reversible by antibody treatment. Science 364(6442) (2019)
...Van Gorp H, De Haard H, Blanchetot C, Saunders M, Hammad H, Savvides SN, Lambrecht BN
RgGuinier 2.1 nm
Dmax 8.2 nm
VolumePorod 46 nm3

SASDF96 – Olfactomedin-1 BMY isoform

Noelin experimental SAS data
DAMMIF model
Sample: Noelin tetramer, 72 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM HEPES, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 5
Design and structural characterisation of olfactomedin-1 variants as tools for functional studies. BMC Mol Cell Biol 20(1):50 (2019)
...van den Hoek H, Janssen BJC
RgGuinier 5.4 nm
Dmax 16.3 nm
VolumePorod 160 nm3

SASDJA6 – Complement C5

Complement C5 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Complement C5 monomer, 186 kDa Homo sapiens protein
Buffer: 20mM Tris pH, 75mM NaCl, and 3% glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 12
The allosteric modulation of Complement C5 by knob domain peptides. Elife 10 (2021)
...van den Elsen JM
RgGuinier 4.8 nm
Dmax 17.6 nm
VolumePorod 392 nm3

SASDKY6 – ParD2 antitoxin from Vibrio cholerae in low salt pH 8

Antitoxin ParD experimental SAS data
Antitoxin ParD Kratky plot
Sample: Antitoxin ParD dodecamer, 108 kDa Vibrio cholerae serotype … protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Jul 18
Entropic pressure controls the oligomerization of the Vibrio cholerae ParD2 antitoxin Acta Crystallographica Section D Structural Biology 77(7):904-920 (2021)
...Van Dyck J, Sobott F, Versées W, Charlier D, Loris R
RgGuinier 3.4 nm
Dmax 13.9 nm
VolumePorod 190 nm3

SASDL97 – Ru-MtrCAB complex formed by reconstitution of Ru-MtrC and MtrAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrAExtracellular iron oxide respiratory system surface decaheme cytochrome c component MtrCExtracellular iron oxide respiratory system outer membrane component MtrB experimental SAS data
DAMMIN model
Sample: Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 77 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2020 Jan 13
Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit Frontiers in Microbiology 12 (2021)
...van Wonderen J, Casadevall C, Martel A, Jeuken L, Reisner E, Clarke T, Butt J
RgGuinier 4.7 nm
Dmax 16.6 nm
VolumePorod 154 nm3

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data
BUNCH model
Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 4.0 nm
Dmax 16.0 nm
VolumePorod 175 nm3

SASDLA7 – Outer membrane protein complex MtrCAB

Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrAExtracellular iron oxide respiratory system outer membrane component MtrBExtracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC experimental SAS data
DAMMIN model
Sample: Extracelllular iron oxide respiratory system periplasmic decaheme cytochrome c component MtrA monomer, 39 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system outer membrane component MtrB monomer, 75 kDa Shewanella oneidensis (strain … protein
Extracellular iron oxide respiratory system surface decaheme cytochrome c component MtrC monomer, 75 kDa Shewanella oneidensis (strain … protein
Buffer: 20 mM HEPES, 100 mM NaCl, 2.8 mM Fos-choline 12, 13% D2O, pH: 7.8
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Sep 12
Bespoke Biomolecular Wires for Transmembrane Electron Transfer: Spontaneous Assembly of a Functionalized Multiheme Electron Conduit Frontiers in Microbiology 12 (2021)
...van Wonderen J, Casadevall C, Martel A, Jeuken L, Reisner E, Clarke T, Butt J
RgGuinier 5.2 nm
Dmax 17.1 nm
VolumePorod 234 nm3

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data
OTHER model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 3.4 nm
Dmax 10.7 nm
VolumePorod 122 nm3

SASDAC7 – Complex LytTR-comcde

comcdeResponse regulator experimental SAS data
SASREF model
Sample: comcde , 24 kDa Streptococcus pneumoniae DNA
Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jun 2
Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
...van Tilbeurgh H, Durand D, Quevillon-Cheruel S
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 74 nm3

SASDEL7 – Human glycine decarboxylase

Glycine decarboxylase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 3.9 nm
VolumePorod 304 nm3

SASDEM7 – Human glycine decarboxylase with glycine

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT , 100 mM glycine, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 315 nm3

SASDSM7 – Full-length Heat shock factor 2-binding protein (HSF2BP)

Heat shock factor 2-binding protein experimental SAS data
Heat shock factor 2-binding protein Kratky plot
Sample: Heat shock factor 2-binding protein tetramer, 150 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl pH 7.5, 250 mM NaCl, and 5 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Oct 2
BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. Sci Adv 9(43):eadi7352 (2023)
...van Rossum-Fikkert SE, Legrand P, Ouldali M, Winter JM, Ropars V, David G, Arteni AA, Wyman C, Knipscheer P, Kanaar R, Zelensky AN, Zinn-Justin S
RgGuinier 8.5 nm
Dmax 25.0 nm
VolumePorod 364 nm3

SASDEN7 – Neanderthal glycine decarboxylase

Glycine decarboxylase experimental SAS data
Glycine decarboxylase Kratky plot
Sample: Glycine decarboxylase dimer, 219 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM TRIS pH 7.5, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 12
Glycine decarboxylase (P-protein of glycine cleavage system)
Bart Van Laer
RgGuinier 4.0 nm
VolumePorod 309 nm3

SASDLN7 – 1:1 complex of Ferredoxin-dependent glutamate synthase 2 (FdGlts) with ferredoxin

Ferredoxin-dependent glutamate synthase 2Ferredoxin-1 experimental SAS data
OTHER model
Sample: Ferredoxin-dependent glutamate synthase 2 monomer, 169 kDa Synechocystis sp. (strain … protein
Ferredoxin-1 monomer, 11 kDa Nostoc sp. (strain … protein
Buffer: Hepes– KOH buffer, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2002 Jun 4
The Active Conformation of Glutamate Synthase and its Binding to Ferredoxin Journal of Molecular Biology 330(1):113-128 (2003)
van den Heuvel R, Svergun D, Petoukhov M, Coda A, Curti B, Ravasio S, Vanoni M, Mattevi A
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 262 nm3

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag

his-trx-myotilin experimental SAS data
Full-length myotilin with N-terminally fused thioredoxin tag Rg histogram
Sample: his-trx-myotilin monomer, 70 kDa Homo sapiens protein
Buffer: 20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 5.1 nm
Dmax 16.7 nm
VolumePorod 131 nm3

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452)

Myotilin Ig1Ig2 (220-452) experimental SAS data
Myotilin Ig1Ig2 (220-452) Kratky plot
Sample: Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2010 Nov 16
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.7 nm
Dmax 15.1 nm
VolumePorod 52 nm3

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444)

Myotilin Ig1Ig2 (250-444) experimental SAS data
Myotilin Ig1Ig2 (250-444) Kratky plot
Sample: Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 2.8 nm
Dmax 10.1 nm
VolumePorod 29 nm3

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data
Myotilin Ig1Ig2 (250-498) Kratky plot
Sample: Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.2 nm
Dmax 14.0 nm
VolumePorod 37 nm3

SASDNB8 – Mature full length human prion protein

Major prion protein experimental SAS data
Mature full length human prion protein Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.2 nm

SASDNC8 – Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP]

Major prion protein experimental SAS data
Mature full length human prion protein in the presence of zinc-tetracationic porphyrin [Zn(II)-BnPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 3.1 nm
Dmax 11.3 nm

SASDKD8 – LuxR family transcriptional regulator, SmcR

LuxR family transcriptional regulator experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator , 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, ...vanced Photon Source (APS), Argonne National Laboratory on 2020 Aug 8
The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences. Nucleic Acids Res 49(10):5967-5984 (2021)
...van Kessel JC
RgGuinier 3.1 nm
Dmax 12.0 nm
VolumePorod 67 nm3

SASDND8 – Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP]

Major prion protein experimental SAS data
Mature full length human prion protein in the presence of a cationic Fe(III) porphyrin [Fe(III)-TMPyP] Rg histogram
Sample: Major prion protein monomer, 23 kDa Homo sapiens protein
Buffer: 10 mM HEPES 100 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2020 Dec 13
A tetracationic porphyrin with dual anti-prion activity. iScience 26(9):107480 (2023)
...Vanni I, Nonno R, Di Bari MA, Tully MD, Cecatiello V, Ciossani G, Pasqualato S, Van Anken E, Salmona M, Castilla J, Requena JR, Banfi S, Musco G, Chiesa R
RgGuinier 2.8 nm
Dmax 10.3 nm

SASDKE8 – LuxR family transcriptional regulator, SmcR N55I mutant

LuxR family transcriptional regulator, N55I experimental SAS data
CHIMERA model
Sample: LuxR family transcriptional regulator, N55I , 52 kDa Vibrio vulnificus protein
Buffer: 200 mM NaCl, 25 mM Tris, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, ...vanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 11
The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences. Nucleic Acids Res 49(10):5967-5984 (2021)
...van Kessel JC
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 45 nm3

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

Myotilin (222-452) experimental SAS data
Myotilin (222-452) Kratky plot
Sample: Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 36 nm3

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

Myotilin (222-452) R405K experimental SAS data
Myotilin (222-452) R405K Kratky plot
Sample: Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
...van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 36 nm3

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data
EKC/KEOPS complex subunit GON7 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G
RgGuinier 3.1 nm
Dmax 12.5 nm
VolumePorod 46 nm3

SASDFL8 – GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3Probable tRNA N6-adenosine threonylcarbamoyltransferase experimental SAS data
OTHER model
Sample: EKC/KEOPS complex subunit GON7 monomer, 12 kDa Homo sapiens protein
EKC/KEOPS complex subunit LAGE3 monomer, 15 kDa Homo sapiens protein
Probable tRNA N6-adenosine threonylcarbamoyltransferase monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 May 12
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G
RgGuinier 3.1 nm
Dmax 11.5 nm
VolumePorod 91 nm3

SASDFM8 – GON7, the fifth subunit of human KEOPS, bound to the EKC/KEOPS complex subunit, LAGE3

EKC/KEOPS complex subunit GON7EKC/KEOPS complex subunit LAGE3 experimental SAS data
EKC/KEOPS complex subunit GON7 EKC/KEOPS complex subunit LAGE3 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 dimer, 25 kDa Homo sapiens protein
EKC/KEOPS complex subunit LAGE3 dimer, 33 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 19
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
...van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G
RgGuinier 3.6 nm
Dmax 15.5 nm
VolumePorod 126 nm3

SASDP39 – apo Saccharomyces cerevisiae Arginyltransferase-1

Arginyl-tRNA--protein transferase 1 experimental SAS data
ALPHAFOLD model
Sample: Arginyl-tRNA--protein transferase 1 monomer, 60 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 2% glycerol, 100 mM KCl, 50 mM Tris-HCl, 1mM DTT, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), ...vanced Light Source (ALS) on 2021 May 12
The Structure of Saccharomyces cerevisiae Arginyltransferase 1 (ATE1). J Mol Biol 434(21):167816 (2022)
Van V, Ejimogu NE, Bui TS, Smith AT
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 105 nm3

SASDA89 – Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorGranulocyte-macrophage colony-stimulating factor experimental SAS data
NONE model
Sample: GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein
Granulocyte-macrophage colony-stimulating factor dimer, 29 kDa Ovis aries protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Sep 10
Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF. Nat Commun 7:13228 (2016)
...van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN
RgGuinier 3.8 nm
Dmax 12.4 nm
VolumePorod 231 nm3

SASDA99 – Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorInterleukin-2 experimental SAS data
NONE model
Sample: GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein
Interleukin-2 monomer, 16 kDa Ovis aries protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Sep 10
Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF. Nat Commun 7:13228 (2016)
...van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN
RgGuinier 4.1 nm
Dmax 12.9 nm
VolumePorod 253 nm3

SASDB99 – Extracellular assembly of the human TSLP:TSLPR:IL-7Ralpha complex

Thymic stromal lymphopoietinInterleukin-7 receptor subunit alphaCytokine receptor-like factor 2 experimental SAS data
ALLOSMOD model
Sample: Thymic stromal lymphopoietin monomer, 15 kDa Homo sapiens protein
Interleukin-7 receptor subunit alpha monomer, 26 kDa Homo sapiens protein
Cytokine receptor-like factor 2 monomer, 24 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 8
Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma. Nat Commun 8:14937 (2017)
...Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Lambrecht BN, Hammad H, De Winter H, Beyaert R, Lippens G, Savvides SN
RgGuinier 3.1 nm
Dmax 10.3 nm
VolumePorod 98 nm3

SASDQB9 – YdaT_toxin domain-containing protein

YdaT_toxin domain-containing protein experimental SAS data
OTHER [STATIC IMAGE] model
Sample: YdaT_toxin domain-containing protein tetramer, 74 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 16
Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
...Van Dyck J, Bervoets I, Charlier D, Loris R
RgGuinier 3.5 nm
Dmax 12.0 nm
VolumePorod 130 nm3

SASDQC9 – Mutant YdaT_toxin domain-containing protein (L111N/F118R)

YdaT_toxin domain-containing protein (mutant: L111N, F118R) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: YdaT_toxin domain-containing protein (mutant: L111N, F118R) monomer, 18 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Apr 14
Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
...Van Dyck J, Bervoets I, Charlier D, Loris R
RgGuinier 2.4 nm
Dmax 8.2 nm
VolumePorod 32 nm3

SASDED9 – Relaxase domain of TraI

Relaxase (Tra_2) domain of TraI experimental SAS data
GASBOR model
Sample: Relaxase (Tra_2) domain of TraI monomer, 46 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...van der Does C
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 61 nm3

SASDQD9 – N-terminal domain of YdaT_toxin domain-containing protein

YdaT_toxin domain-containing protein experimental SAS data
OTHER [STATIC IMAGE] model
Sample: YdaT_toxin domain-containing protein monomer, 13 kDa Escherichia coli O157:H7 protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Apr 16
Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
...Van Dyck J, Bervoets I, Charlier D, Loris R
RgGuinier 1.7 nm
Dmax 5.7 nm
VolumePorod 29 nm3

SASDEE9 – TraI_2_C domain of TraI

TraI_2_C domain of TraI experimental SAS data
GASBOR model
Sample: TraI_2_C domain of TraI monomer, 21 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...van der Does C
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDQE9 – YdaT_toxin domain-containing protein bound to 30 base pair dsDNA

YdaT_toxin domain-containing proteinOm 30 base pair dsDNA experimental SAS data
OTHER model
Sample: YdaT_toxin domain-containing protein tetramer, 74 kDa Escherichia coli O157:H7 protein
Om 30 base pair dsDNA dimer, 37 kDa DNA
Buffer: 20 mM Tris-HCl, 200 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2021 Apr 14
Structural basis of DNA binding by YdaT, a functional equivalent of the CII repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7 Acta Crystallographica Section D Structural Biology 79(3):245-258 (2023)
...Van Dyck J, Bervoets I, Charlier D, Loris R
RgGuinier 4.2 nm
Dmax 12.8 nm
VolumePorod 190 nm3

SASDEF9 – TraI of Neisseria gonorrhoeae

TraI experimental SAS data
GASBOR model
Sample: TraI monomer, 91 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 5
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
...van der Does C
RgGuinier 7.3 nm
Dmax 31.4 nm
VolumePorod 293 nm3

SASDHG9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - apo form

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.5 nm
Dmax 8.8 nm
VolumePorod 77 nm3

SASDHH9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 76 nm3

SASDHJ9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and trehalose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM trehalose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.3 nm
VolumePorod 76 nm3

SASDHK9 – Trehalose transferase (TreT) from Thermoproteus uzoniensis - with magnesium and UDP-glucose

Trehalose transferase (Trehalose phosphorylase/synthase) experimental SAS data
DAMMIN model
Sample: Trehalose transferase (Trehalose phosphorylase/synthase) monomer, 47 kDa Thermoproteus uzoniensis protein
Buffer: 50 mM HEPES, 100 NaCl, 4 mM DTT, 20 mM MgCl2, 1 mM UDP-glucose, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Sep 27
Anomeric Selectivity of Trehalose Transferase with Rare L-Sugars ACS Catalysis (2020)
...van der Eijk H, Laustsen J, Jeffries C, Svergun D, Hagedoorn P, Bento I, Hanefeld U
RgGuinier 2.4 nm
Dmax 8.5 nm
VolumePorod 73 nm3

SASDK42 – Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data

Chitin-binding protein CbpD experimental SAS data
OTHER model
Sample: Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer: 15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Oct 1
The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
...van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G
RgGuinier 3.2 nm
Dmax 12.0 nm
VolumePorod 64131 nm3

SASDRH5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein monomer, 11 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2021 Dec 12
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.6 nm
Dmax 5.3 nm
VolumePorod 12 nm3

SASDAA9 – EcPaaA2-EcParE2His construct

Plasmid stabilization protein ParE Uncharacterized protein (Antitoxin) experimental SAS data
CRYSOL model
Sample: Plasmid stabilization protein ParE 16-mer, 188 kDa Escherichia coli protein
Uncharacterized protein (Antitoxin) 16-mer, 135 kDa Escherichia coli protein
Buffer: 50 mM Tris-HCl 500 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 9
A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. J Mol Biol 428(8):1589-603 (2016)
...Van Melderen L, Loris R
RgGuinier 3.8 nm
Dmax 16.2 nm
VolumePorod 312 nm3

SASDRJ5 – A ssr1698 Dri1 hemoprotein, wild-type (as-purified) variant + heme

Ssr1698 protein experimental SAS data
Sample: Ssr1698 protein dimer, 23 kDa Synechocystis sp. (strain … protein
Buffer: 50 mM Hepes, 200 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 16-ID (LiX), National Synchrotron Light Source II (NSLS-II) on 2022 Jun 13
A hemoprotein with a zinc-mirror heme site ties heme availability to carbon metabolism in cyanobacteria. Nat Commun 15(1):3167 (2024)
...van Dam H, Yang L, Blaby IK, Blaby-Haas CE
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 26 nm3